Ror1 antibody immunoconjugates

ABSTRACT

Provided herein are immunoconjugates comprising an anti-ROR1 antibody or an antigen-fragment fragment thereof and a drug moiety. These immunoconjugates are useful for treating ROR1 expressing cancers.

CROSS-REFERENCE TO RELATED APPLICATIONS

This application is a continuation of U.S. patent application Ser. No. 16/027,967, filed on Jul. 5, 2018, which is a continuation of U.S. patent application Ser. No. 16/016,238, filed on Jun. 22, 2018, which claims priority from U.S. Patent Applications 62/524,382, 62/524,386, and 62/524,388, all of which were filed on Jun. 23, 2017. The disclosures of these priority applications are incorporated by reference herein in their entirety.

SEQUENCE LISTING

The instant application contains a Sequence Listing which has been submitted electronically in ASCII format and is hereby incorporated by reference in its entirety. Said ASCII copy, created on Apr. 4, 2019, is named 024651_C2002_SL.txt and is 56,726 bytes in size.

BACKGROUND OF THE INVENTION

Cancer is the second leading cause of human death next to coronary artery disease. Receptor tyrosine kinases (RTKs) play a key role in oncogenic transformation, growth and metastases. RTKs regulate cell differentiation, proliferation, migration, angiogenesis, and survival. The receptor tyrosine kinase-like orphan receptor 1 (ROR1) is an evolutionarily-conserved type I membrane protein that belongs to the ROR subfamily and has extracellular domains that contain immunoglobulin (Ig)-like, Frizzled, and Kringle domains. ROR1-deficient mice display a variety of phenotypic defects within the skeletal and urogenital systems, as well as postnatal growth retardation. ROR1 is expressed during embryogenesis and by a variety of different cancers, but not by normal post-partum tissues, and can be considered an onco-embryonic surface antigen. Functional data suggest that ROR1 may function in non-canonical WNT-signaling to promote the survival of malignant cells.

ROR1 expression and activation appears to be correlated with features of tumor aggressiveness in models of chronic lymphocytic leukemia (CLL), breast cancer, lung cancer, gastric cancer, and melanoma (Li et al., PLoS One 5(7):e11859 (2010); Gentile et al., Cancer Res. 71(8):3132-41 (2011); Zhang et al., PLoS One 7(3):e31127 (2012); Yamaguchi et al., Cancer Cell. 21(3):348-61 (2012); Daneshmanesh et al., Leukemia 26(6):1348-55 (2012); Daneshmanesh et al., Leuk Lymphoma 54(4):843-50 (2013); O'Connell et al., Cancer Discov. 3(12):1378-93 (2013); Hojjat-Farsangi et al., PLoS One 8(4):e61167 (2013); Hojjat-Farsangi et al., PLoS One 8(10):e78339 (2013); Ida et al., Cancer Sci. 107(2):155-61 (2016); and Janovska et al., Clin Cancer Res. 22(2):459-69 (2016)). Elevated levels of ROR1 expression in patients and cell lines are associated with genes involved in epithelial-mesenchymal transition (EMT) (Cui et al., Cancer Res. 73(12):3649-60 (2013)). In patients with CLL, high levels of ROR1 expression are associated with shorter treatment-free survival and overall survival (OS) (Cui et al., Blood 128(25):2931-2940 (2016)). Similarly, in patients with ovarian cancer, high ROR1 expression is associated with poor clinical outcomes (Zhang et al., Sci Rep. 4:5811 (2014)).

In view of the role of ROR1 in cancer, there is a need for new and improved therapies that target ROR1-positive cancer cells.

SUMMARY OF THE INVENTION

Provided herein is an immunoconjugate having the formula of Ab-((L)m-(D))n, wherein: Ab is an antibody or an antigen-binding fragment thereof that specifically binds to human receptor tyrosine kinase like orphan receptor 1 (ROR1); L is a linker, and m is 0 or 1; D is a cytotoxic drug moiety; and n is an integer from 1 to 10.

The cytotoxic drug moiety may be selected from the group consisting of, for example, an anti-tubulin agent, a DNA alkylating agent, a DNA cross-linking agent, a DNA intercalating agent, and an RNA polymerase II inhibitor. In some embodiments, the cytotoxic drug moiety is selected from the group consisting of monomethyl auristatin E (MMAE), azonafide, α-amanitin, duocarmycin TM, pyrrolobenzodiazepine (PBD), PNU-159682, and pharmaceutically acceptable salts, esters, and analogs thereof.

The linker in the immunoconjugate may comprise a cleavable moiety. It may be cleaved inside a target cell. Alternatively, the linker is not cleavable. The linker can be branched or unbranched. In some embodiments, the linker comprises one or more moieties selected from valine-citrulline (VC), valine-alanine (VA), para-aminobenzyloxycarbonyl (PAB), polyethylene glycol (PEG), diaminopropionic acid (DPR), Phe-C4, C2-Gly3, C6 alkyl, dimethylethylamine (DMEA), and ethylene diamine (EDA). In certain embodiments, the linker is covalently bonded to the antibody or antigen-binding fragment at a succinimide, a carbonyl, or a cyclooctene, or a triazole group of the linker.

In certain embodiments, the antibody or fragment in the immunoconjugate is covalently bonded to the linker by reaction with a moiety selected from the group consisting of 6-maleimidocaproyl (MC)-VC-PAB; 6-MC-C₆; 6-MC-PEG4-VC-PAB-DMEA; 6-MC-PEG4-VA; 6-MC-DPR-VC-PAB; 6-MC-Phe-C₄-VC-PAB; 6-MC-Phe-C₄-VC-PAB-DMEA; 6-MC-C2-Gly3-EDA; dibenzylcyclooctyne (DBCO)-(PEG2-VC-PAB)₂; DBCO-PEG4-VC-PAB-DMEA; and N-succinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate-VC-PAB. As used herein, VC represents a valine-citrulline dipeptide; VA represents a valine-alanine dipeptide; PEG represents polyethylene glycol; PAB represents para-amino-benzyloxycarbonyl; DMEA represents dimethylethylamine; Phe represents a benzyl group; and EDA represents ethylene diamine.

Provided herein also is an immunoconjugate having the formula of Ab-((L)m-(D))n, wherein: Ab is an antibody or an antigen-binding fragment thereof that specifically binds to human receptor tyrosine kinase like orphan receptor 1 (ROR1); L is a cleavable linker, and m is 0 or 1; D is an auristatin (e.g., MMAE); and n is an integer from 1 to 10.

In an immunoconjugate of the present disclosure, the linker may comprise, for example, a heterocycle or carbonyl covalently bonded to the antibody or antigen-binding fragment, a spacer group covalently bonded to the heterocycle or carbonyl, and an ester, thioester, amide, carbonate, thiocarbonate or carbamate covalently bonded to the cytotoxic drug moiety. In some embodiments, the spacer group comprises an amino acid, a polyamino acid, or an amino benzyl group, or a combination thereof. In some embodiments, the linker in an immunoconjugate of the present disclosure forms a covalent bond with a cysteine or lysine residue on the antibody or fragment.

The Ab (antibody or fragment thereof) component of an immunoconjugate of the present disclosure may bind to the same ROR1 epitope as an antibody comprising the heavy chain and light chain amino acid sequences of SEQ ID NOs: 3 and 4, respectively. The antibody or fragment may comprise the heavy chain complementarity-determining region (CDR) 1-3 (HCDR1-3) in SEQ ID NO: 3 and the light chain CDR1-3 (LCDR1-3) in SEQ ID NO: 4. In some embodiments, the antibody or fragment comprises the amino acid sequences of SEQ ID NOs: 7-9, and the light chain of the antibody comprises the amino acid sequences of SEQ ID NOs: 10-12. The antibody or fragment may be humanized. The antibody or fragment may have one or more of the following properties: a) facilitates ROR1 internalization in a human cell; b) binds to human ROR1 with a K_(D) of less than 100 nM (e.g., less than 50, 40, 30, 20, or 10 nM); and c) inhibits growth of ROR1⁺ human cancer cells in vitro with an EC₅₀ of 500 nM or less (e.g., 400 nM or less, 300 nM or less, 200 nM or less, or 100 nM or less).

In some embodiments, the heavy chain variable domain (V_(H)) and light chain variable domain (V_(L)) of the antibody in the immunoconjugate comprise the amino acid sequences of: a) SEQ ID NOs: 5 and 6, respectively; b) SEQ ID NOs: 5 and 50, respectively; c) SEQ ID NOs: 48 and 6, respectively; or d) SEQ ID NOs: 48 and 50, respectively. The antibody may comprise a human IgG₁ constant region and optionally also a human κ light chain constant region. In further embodiments, the heavy chain and light chain of the antibody comprise the amino acid sequences of: a) SEQ ID NOs: 3 and 4, respectively; b) SEQ ID NOs: 3 and 49, respectively; c) SEQ ID NOs: 47 and 4, respectively; or d) SEQ ID NOs: 47 and 49, respectively.

In some embodiments, the Ab component of the immunoconjugate is an Fab, F(ab)₂, or scFv, e.g., an Fab, F(ab)₂, or scFv.

Specific embodiments of the present disclosure include an immunoconjugate comprising an antibody conjugated to a cytotoxic drug moiety, wherein the V_(H) and V_(L) of the antibody comprise the amino acid sequences of SEQ ID NOs: 5 and 6, respectively. Examples of such an immunoconjugate are shown in Tables 2 and 3 below, and include Antibody-Drug Conjugates (ADC)-A, E, H, I, J, K, L, M, N, O, P, Q, and R. In further embodiments, the heavy chain and light chain of the antibody comprise the amino acid sequences of SEQ ID NOs: 3 and 4, respectively.

In the immunoconjugate of the present disclosure, the number of the drug moiety to per antibody or fragment, or the ratio of the cytotoxic drug moiety to the antibody or fragment (DAR), may be 1 to 10, for example, 1 to 7, 1 to 6, 1 to 5, 2 to 7, 2 to 6, or 2 to 5.

Also provided herein are pharmaceutical compositions comprising an immunoconjugate of the present disclosure and a pharmaceutically acceptable excipient. The pharmaceutical compositions may further comprise an additional therapeutic agent selected from the group consisting of a Bruton's tyrosine kinase (BTK) inhibitor, a B-cell lymphoma 2 (Bcl-2) inhibitor, a mammalian target of rapamycine (mTOR) inhibitor, and a phosphoinositide 3-kinase (PI3K) inhibitor. For example, the additional therapeutic agent is selected from ibrutinib, acalabrutinib, venetoclax, everolimus, sapanisertib, and idelalisib.

Also provided herein is a therapy or method for treating cancer in a patient in need thereof, comprising administering to the patient a therapeutically effective amount of an immunoconjugate of the present invention. The cancer may be homogenous or heterogeneous for ROR1 expression and may be, for example, a leukemia, a lymphoma, or a solid tumor. In some embodiments, the cancer is chronic lymphocytic leukemia (CLL), T-cell leukemia (TCL), mantle cell lymphoma (MCL), diffuse large B-cell lymphoma (DLBCL), Burkitt's lymphoma, multiple myeloma (MM), marginal zone lymphoma (MZL), small lymphocytic lymphoma (SLL), or a non-Hodgkin lymphoma (NHL) that has undergone Richter's transformation. In some embodiments, the cancer is non-small cell lung cancer (NSCLC), hepatocellular carcinoma, pancreatic cancer, osteosarcoma, head and neck cancer, ovarian cancer, breast cancer, or triple negative breast cancer (TNBC).

The therapy or treatment method of the present disclosure may further comprise administering to the patient an additional anti-cancer therapeutic agent, which may be, for example, a Bruton's tyrosine kinase (BTK) inhibitor, a B-cell lymphoma 2 (Bcl-2) inhibitor, a mammalian target of rapamycine (mTOR) inhibitor, and a phosphoinositide 3-kinase (PI3K) inhibitor. In some embodiments, the additional therapeutic agent is selected from ibrutinib, acalabrutinib, venetoclax, everolimus, sapanisertib, and idelalisib.

In certain embodiments of the present therapy or treatment method, the cancer is CLL, MCL, or an NHL that has undergone Richter's transformation.

Provided herein also are immunoconjugates and pharmaceutical compositions as described herein for use in treating cancer in the therapy or treatment methods described herein. For example, provided herein is an immunoconjugate having the formula of Ab-((L)m-(D))n for use in treating cancer in a patient in need thereof, wherein: Ab is an antibody or an antigen-binding fragment thereof that specifically binds to human receptor tyrosine kinase like orphan receptor 1 (ROR1); L is a linker, and m is 0 or 1; D is a cytotoxic drug moiety; and n is an integer from 1 to 10. Exemplary embodiments of the immunoconjugate and the treatment are described above and will be further described below.

Provided herein also are the use of an immunoconjugate herein for the manufacture of a medicament for use in treating cancer in a patient in need thereof. For example, provided herein is the use of is an immunoconjugate having the formula of Ab-((L)m-(D))n for the manufacture of a medicament in treating cancer in a patient in need thereof, wherein: Ab is an antibody or an antigen-binding fragment thereof that specifically binds to human receptor tyrosine kinase like orphan receptor 1 (ROR1); L is a linker, and m is 0 or 1; D is a cytotoxic drug moiety; and n is an integer from 1 to 10. Exemplary embodiments of the immunoconjugate and the treatment are described above and will be further described below.

The present disclosure also provides a method of making an immunoconjugate, comprising: providing an antibody or an antigen-binding fragment thereof that specifically binds to human receptor tyrosine kinase like orphan receptor 1 (ROR1); conjugating to the antibody a cytotoxic drug moiety selected from the group consisting of an anti-tubulin agent, a DNA alkylating agent, a DNA cross-linking agent, a DNA intercalating agent, and an RNA polymerase II inhibitor; wherein the heavy chain of the antibody comprises the amino acid sequences of SEQ ID NOs: 7-9, and the light chain of the antibody comprises the amino acid sequences of SEQ ID NOs: 10-12. Exemplary embodiments of the immunoconjugate are described above and will be further described below.

Provided herein also are articles of manufactures, such as kits, comprising an immunoconjugate of the present disclosure.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 is a schematic diagram illustrating a non-limiting example of an immunoconjugate of the present disclosure.

FIGS. 2A and 2B are graphs illustrating the binding of various concentrations of Ab1 and ADC-A to ROR1-positive cells Jeko-1 (2A) and MDA-MB-231 (2B). The EC₅₀ values for Ab1 and ADC-A are shown below each graph. The similarity between the EC₅₀ values for unconjugated Ab1 and ADC-A demonstrates that drug conjugation had minimal impact on Ab1's binding to the target cells.

FIGS. 3A and 3B are graphs illustrating the binding of Ab1, 4A5, ADC-A and ADC-T (3A) and Ab1, ADC-A, D10 and ADC-S (3B) to Jeko-1 cells. The EC₅₀ values for the antibodies and immunoconjugates are shown below each graph. The similarity between the EC₅₀ values of unconjugated antibodies and the corresponding ADC constructs demonstrates that drug conjugation had minimal impact on the antibodies' binding to the target cells. The difference in EC₅₀ values between Ab1/ADC-A and D10/ADC-S reflect the higher affinity of Ab1 for ROR1 as compared to D10.

FIGS. 4A and 4B are graphs illustrating the internalization of Ab1, ADC-A, and ADC-B into Jeko-1 cells (4A), and the internalization of Ab1 and ADC-A into MDA-MB-231 cells (4B). The addition of linker and payload to Ab1 did not negatively impact its binding or internalization, as demonstrated with ADC-A and ADC-B.

FIG. 5 is a graph illustrating the internalization rate of Ab1 in MDA-MB-231 cells. The graph shows an initially rapid rate, and then a slower rate, of cell surface receptor clearance.

FIG. 6 is a graph illustrating the cell surface expression of ROR1 during Ab1 internalization into Jeko-1 cells. While Ab1 is rapidly internalized, quantitation of cell surface ROR1 shows a small decrease in the first 10 minutes, with subsequent measurements indicating restoration of ROR1 surface expression to initial or slightly higher levels.

FIGS. 7A-C are graphs illustrating cell surface expression of ROR1 during Ab1 internalization on Jeko-1 cells (7A), MDA-MB-468 cells (7B), and MDA-MB-231 cells (7C).

FIGS. 8A-8I are representative IC₅₀ plots showing ROR1 binding by immunoconjugates of the present disclosure, as well as unconjugated MMAE, in cancer cell lines TMD-8 (8A), HBL-1 (8B), DOHH2 (8C), MDA-MB-468 (8D), Bt549 (8E), TOV112D (8F), JHOM1 (8G), SKOvr3 (8H), and Mino (8I).

FIG. 9 is a graph illustrating the inhibition of cell proliferation by 3, 10, or 30 μg/mL of ADC-A in Jeko-1 cells, with or without pre-treatment with 100 μg/mL Ab1. ADC-A inhibited cell proliferation in a dose-dependent matter. Pre-incubation of the cells with Ab1 reduced this activity, demonstrating that ADC-A's inhibitory activity on cell proliferation was mediated by the binding of ADC-A to ROR1.

FIG. 10 is a graph illustrating the dose-dependent inhibition of leukemic cell tumor burden in a TCL1-ROR1 chronic lymphocytic leukemia mouse model upon treatment with vehicle, 10 mg/kg Ab1, or 1 mg/kg, 2 mg/kg, or 5 mg/kg ADC-A.

FIG. 11 is a graph illustrating tumor growth inhibition in an MCL xenograft model upon treatment with vehicle, 5 mg/kg ADC-A or ADC-Q intravenously (IV) every four days (Q4D), 10 mg/kg Ab1 IV once per week (QW), or 20 mg/kg ibrutinib per os (PO) every day (QD). ADC-A treatment caused tumor regression.

FIG. 12 is a pair of graphs showing ROR1 expression (left panel) and tumor growth inhibition in a DLBCL-GCB xenograft mouse model upon treatment with control, 10 mg/kg QW Ab1, 50 mg/kg venetoclax QD, Ab1+venetoclax, or 5 mg/kg ADC-A QW (right panel). ADC-A treatment resulted in complete tumor regression in all animals treated. Ab1 alone, venetoclax alone, and a combination of Ab1 and venetoclax were ineffective.

FIG. 13 is a set of graphs showing ROR1 expression (left panel) and inhibition of tumor growth upon treatment with vehicle, 2.5 or 5 mg/kg ADC-A, or 10 mg/kg Ab1 (right panel), in a chemotherapy-resistant Richter's transformation xenograft mouse model. Although only 20-30% of the intra-tumoral cells were ROR1-positive, complete and sustained tumor regressions were observed with 5 mg/kg ADC-A.

FIG. 14 is a graph illustrating tumor growth inhibition upon treatment with vehicle, 1 or 5 mg/kg ADC-A IV QW, 1 or 5 mg/kg ADC-B IV QW, or 10 mg/kg Ab1 IV QW in a MDA-MB-231 triple negative breast cancer (TNBC) mammary fat pad xenograft mouse model.

FIG. 15 is a graph illustrating tumor growth inhibition upon treatment with vehicle, 1 or 5 mg/kg ADC-A IV Q4D, or 10 mg/kg Ab1 IV QW in a BR5011 human TNBC xenograft mouse model. Although only 58% of the intra-tumoral cells were ROR1-positive, complete and sustained regressions were observed with 5 mg/kg ADC-A, where tumor regression was maintained for at least 28 days after the last dose.

FIG. 16 is a graph illustrating tumor growth inhibition upon treatment with vehicle, 1 or 5 mg/kg ADC-A IV Q4D, or 10 mg/kg Ab1 IV QW in a BR5015 (low ROR1 expression) human TNBC xenograft mouse model. Tumor regression was observed even though only 58% of the intra-tumoral cells were ROR1-positive.

FIG. 17 is a graph illustrating tumor growth inhibition in a Jeko-1 human mantle cell lymphoma xenograft mouse model. Mice were treated with vehicle; 1 mg/kg ADC-N, ADC-P, or ADC-R; or 5 mg/kg ADC-A, ADC-L, ADC-M, ADC-S or ADC-T. Vehicle and ADC constructs were administered IV Q4D. Significant tumor regression was observed in animals treated with ADC-A, ADC-L, ADC-M and ADC-S, while inhibition of tumor growth was observed in animals treated with ADC-N, ADC-P, ADC-R and ADC-T.

FIGS. 18A and 18B are graphs illustrating the combination index of treatment with ADC-A and BTK inhibitors ibrutinib (18A) or ACP-196/acalabrutinib (18B) in various cell lines. ADC-A displayed a synergistic effect with both ibrutinib and ACP-196/acalabrutinib on inhibition of cell proliferation.

FIGS. 19A and 19B are graphs illustrating inhibition of Jeko-1 cell proliferation upon treatment with ADC-A, ibrutinib (“Ib”), or a combination of ADC-A and ibrutinib (19A); or ADC-A, ACP-196/acalabrutinib (“ACP196” or “196”), or a combination of ADC-A and ACP-196/acalabrutinib (19B).

FIGS. 20A-C are graphs illustrating the combination index of treatment with ADC-A and Bcl-2 inhibitor ABT-199/venetoclax (“ABT199”) in various cell lines (20A), or of ADC-A with Bcl-2 inhibitor Bcl-2i-1 or Bcl-2i-2 in Jeko-1 cells (20B) or Mino cells (20C). ADC-A displayed a synergistic effect with ABT-199 on inhibition of both MCL and DLBCL cell proliferation. ADC-A also displayed a synergistic effect with other Bcl-2 inhibitors (Bcl-2i-1 and Bcl-2i-2) on inhibition of Jeko-1 cell proliferation, and displayed an additive effect with both inhibitors on inhibition of Mino cell proliferation.

FIG. 21 is a graph illustrating inhibition of Jeko-1 cell proliferation upon treatment with ADC-A, ABT-199, or a combination of ADC-A and ABT-199.

FIG. 22 is a graph illustrating the combination index of treatment with ADC-A and mTOR1/2 inhibitor INK128/sapanisertib (“INK128”) in various cell lines. ADC-A displayed a synergistic effect with INK128 on inhibition of both MCL and DLBCL cell proliferation.

FIG. 23 is a graph illustrating inhibition of Jeko-1 cell proliferation upon treatment with ADC-A, INK128, or a combination of ADC-A and INK128.

FIG. 24 is a graph illustrating the combination index of treatment with ADC-A and PI3K inhibitor CAL-101/idelalisib (“CAL101”) in various cell lines. ADC-A displayed a synergistic effect with CAL101 on inhibition of both MCL and DLBCL cell proliferation.

FIGS. 25A and 25B are graphs illustrating inhibition of cell proliferation upon treatment with ADC-A, PI3K inhibitor CAL-101/idelalisib (“CAL101” or “101”), or a combination of ADC-A and CAL101, in DLBCL-ABC cell line TMD-8 (25A) or in DLBCL-GCB cell line DOHH2 (25B).

DETAILED DESCRIPTION OF THE INVENTION

The present invention provides immunoconjugates of the formula Ab-((L)m-(D))n, wherein Ab is an antibody or an antigen-binding fragment thereof that specifically binds to the ROR1 protein; L is a linker; D is a drug moiety that has therapeutic activity in cancer; m is 0 or 1; and n is an integer from 1 to 10. In the formula, the dash “-” denotes a covalent or non-covalent bond. The antibody or fragment includes, but is not limited to, an antibody or antibody fragment that competes with antibody D10 or Ab1 for binding to human ROR1, or binds to the same epitope as D10 or Ab1. The drug moiety includes, but is not limited to, another antibody or an antigen-binding fragment thereof, a polypeptide, a small molecule compound, a nucleic acid molecule such as a small interfering RNA molecule or an antisense molecule. The immunoconjugates of the present invention may be used to treat a variety of cancers such as ROR1-positive cancers.

1. IMMUNOCONJUGATES

An “antibody-drug conjugate,” or “ADC,” or “immunoconjugate” refers to an antibody molecule, or an antigen-binding fragment thereof, that is covalently or non-covalently bonded, with or without a linker, to one or more biologically active molecule(s). The present immunoconjugates comprise antibodies or fragments thereof that are specific for human ROR1 and can thus serve as excellent targeting moieties for delivering the conjugated payloads to ROR1-positive cells. In some embodiments, a ROR1 immunoconjugate provided herein has an equilibrium dissociation constant (K_(D)) of about 1 μM, 100 nM, 50 nM, 40 nM, 30 nM, 20 nM, 10 nM, 5 nM, 2 nM, 1 nM, 0.5 nM, 0.1 nM, 0.05 nM, 0.01 nM, or 0.001 nM or less (e.g., 10⁻⁸ M or less, from 10⁻⁸ M to 10⁻¹³ M, or from 10⁻⁹ M to 10⁻¹³ M) for human ROR1. K_(D) can be measured by any suitable assay, such as surface plasmon resonance assays (e.g., using a BIACORE®-2000 or a BIACORE®-3000). In certain embodiments, the K_(D) of an immunoconjugate of the invention is less than the K_(D) for the D10 antibody. In certain embodiments, the K_(D) of an immunoconjugate of the invention for human ROR1 is less than about 50, 40, 30, 20, or 10 nM (e.g., 40 nM). In some embodiments, a ROR1 immunoconjugate provided herein inhibits growth of ROR1⁺ human cancer cells in vitro with an EC₅₀ of about 500, 400, 350, 300, or 250 nM or less (e.g., 300 nM or less). As used herein, an antibody is said to bind specifically to an antigen when it binds to the antigen with a K_(D) of 100 nM or less, such as less than 10 nM or less (e.g., 1-5 nM), as determined by, e.g., surface plasmon resonance or Bio-Layer Interferometry.

In certain embodiments, the immunoconjugate provided herein is internalized by a ROR1-positive cell primarily through the lysosome/endosome pathway. In particular embodiments, the internalization is independent of the ROR1 expression level on the cell surface.

Embodiments of the antibody or fragment thereof, the linker, and the drug moiety used in the immunoconjugates are described in further detail below.

1.1. Types and Structures of Antibodies

The term “antibody” is used herein in the broadest sense and includes polyclonal and monoclonal antibodies, such as intact antibodies and functional (antigen-binding) fragments thereof. The term encompasses genetically engineered and/or otherwise modified forms of immunoglobulins, such as intrabodies, peptibodies, chimeric antibodies, fully human antibodies, humanized antibodies, and heteroconjugate antibodies, multi-specific (e.g., bispecific) antibodies, diabodies, triabodies, and tetrabodies, tandem di-scFv, and tandem tri-scFv. Unless otherwise indicated, the term encompasses intact or full-length antibodies, including antibodies of any class or subclass (e.g., IgG and sub-classes thereof such as IgG₁, IgG₂, IgG₃, and IgG₄; IgM; IgE; IgA; and IgD), as well as antibody fragments.

An antibody may include a heavy chain (or a polypeptide sequence derived therefrom) and a light chain (or a polypeptide sequence derived therefrom). The term “variable region” or “variable domain” refers to the domain of an antibody heavy or light chain that is involved in the antibody's binding to an antigen. The variable domains of the heavy chain and light chain (V_(H) and V_(L), respectively) of a native antibody generally have similar structures, with each domain comprising four conserved framework regions and three complementarity-determining regions. A single V_(H) or V_(L) domain may sometimes be sufficient to confer all or a majority of the antigen-binding specificity of an antibody. Furthermore, antibodies that bind a particular antigen may be isolated by using a V_(H) or V_(L) domain from an antibody that binds the antigen to screen a library of complementary V_(L) or V_(H) domains, respectively. See, e.g., Portolano et al., J. Immunol. 150:880-887 (1993); Clarkson et al., Nature 352:624-628 (1991).

The terms “complementarity-determining region” and “CDR,” which are synonymous with “hypervariable region” or “HVR,” refer to subregions within the antibody variable domains, which confer the antibody's specificity and/or affinity for its antigen. In general, there are three CDRs in each heavy chain variable domain (HCDR1, HCDR2, and HCDR3) and three CDRs in each light chain variable domain (LCDR1, LCDR2, and LCDR3). “Framework regions” (“FRs”) refer to the non-CDR portions of the variable domains. In general, there are four FRs in each full-length heavy chain variable domain and four FRs in each full-length light chain variable domain. The precise amino acid sequence boundaries of a given CDR or FR can be readily determined using any of several well-known schemes, including those described by Kabat et al., 5th Ed., Public Health Service, National Institutes of Health, Bethesda, Md. (1991) (“Kabat” numbering scheme); Al-Lazikani et al., JMB 273, 927-948 (1997) (“Chothia” numbering scheme); MacCallum et al., J. Mol. Biol. 262:732-745 (1996) (“contact” numbering scheme); Lefranc et al., Dev Comp Immunol. 27(1):55-77 (2003) (“IMGT” numbering scheme); and Honegger and Pluckthun, J Mol Biol, 309(3):657-70 (2001) (“Aho” numbering scheme).

The boundaries of a given CDR or FR may vary depending on the scheme used for identification. For example, the Kabat scheme is based on sequence alignments, while the Chothia scheme is based on structural information. Numbering for both the Kabat and Chothia schemes is based upon the most common antibody region sequence lengths, with insertions accommodated by insertion letters, for example, “30a.” The two schemes place certain insertions and deletions (“indels”) at different positions, resulting in differential numbering. The contact scheme is based on analysis of complex crystal structures and is similar in many respects to the Chothia numbering scheme. Unless indicated otherwise, the CDRs of the antibodies referred to herein may be identified according to any of the Kabat, Chothia, IMGT, and contact methods.

An antigen-binding fragment of a full-length antibody may be used in making an immunoconjugate of the present invention. Examples of antibody fragments include, but are not limited to, Fv, Fab, Fab′, Fab′-SH, F(ab′)₂; recombinant IgG (rIgG) fragments; diabodies; linear antibodies; single-chain antibody molecules (e.g., scFv or sFv); single domain antibodies (e.g., sdAb, sdFv, nanobodies); and multi-specific antibodies formed from antibody fragments. In certain embodiments, the fragments are single-chain antibody fragments comprising a variable heavy chain region and/or a variable light chain region, such as scFvs.

1.2 Exemplary ROR1 Antibodies

An immunoconjugate of the invention comprises an antibody or an antigen-binding fragment thereof that specifically binds to ROR1, e.g., human ROR1. The antibody or fragment binds to an extracellular portion of the ROR1 protein such as an epitope in one or more of the immunoglobulin (Ig)-like, Frizzled, and Kringle domains of the ROR1 protein. In certain embodiments, the ROR1-binding antibody or fragment binds to an amino acid sequence of ROR1 shown in SEQ ID NO: 1 or 2 (not including the terminal cysteine, which is added for convenience of conjugation) and can be internalized by a ROR1⁺ cell; examples of such an antibody are murine antibodies D10 and 99961. See U.S. Pat. Nos. 9,217,040 and 9,758,591, the disclosures of which are incorporated by reference herein in their entirety. In certain embodiments, the antibody or fragment competes with D10 or 99961 for binding to human ROR1. Amino acid sequences of exemplary anti-ROR1 antibodies used in the immunoconjugates of the invention are shown in Table 1 below, where Ab1-Ab4 are humanized variants of antibody 99961.

TABLE 1 SEQ ID NOs of Exemplary Anti-ROR1 Antibodies Ab HCDR1 HCDR2 HCDR3 VH HC LCDR1 LCDR2 LCDR3 VL LC 99961 7 8 9 45 — 10 11 12 46 — Ab1 7 8 9 5 3 10 11 12 6 4 Ab2 7 8 9 5 3 10 11 12 50 49 Ab3 7 8 9 48 47 10 11 12 6 4 Ab4 7 8 9 48 47 10 11 12 50 49 D10 27 28 29 25 — 30 31 32 26 —

In some embodiments, the antibody or antibody fragment in the immunoconjugate specifically binds human ROR1, and its heavy and light chains respectively comprise: a) the heavy chain CDR1-3 (HCDR1-3) amino acid sequences in SEQ ID NO: 3, and the light chain CDR1-3 (LCDR1-3) amino acid sequences in SEQ ID NO: 4;

-   -   b) HCDR1-3 comprising the amino acid sequences of SEQ ID NO:         7-9, respectively, and LCDR1-3 comprising the amino acid         sequences of SEQ ID NOs: 10-12, respectively;     -   c) the HCDR1-3 amino acid sequences in SEQ ID NO: 13-15, and the         LCDR1-3 amino acid sequences in SEQ ID NOs: 16-18;     -   d) HCDR1-3 comprising the amino acid sequences of SEQ ID NO:         27-29, respectively, and LCDR1-3 comprising the amino acid         sequences of SEQ ID NOs: 30-32, respectively;     -   e) HCDR1-3 comprising the amino acid sequences of SEQ ID NO:         37-39, respectively, and LCDR1-3 comprising the amino acid         sequences of SEQ ID NOs: 40-42, respectively;     -   f) HCDR1-3 comprising residues 26-33, 51-58, and 97-105 of SEQ         ID NO: 5, respectively, and LCDR1-3 comprising residues 27-32,         50-52, and 89-97 of SEQ ID NO: 6, respectively;     -   g) HCDR1-3 comprising residues 26-32, 52-57, and 99-105 of SEQ         ID NO: 5, respectively, and LCDR1-3 comprising residues 24-34,         50-56, and 89-97 of SEQ ID NO: 6, respectively;     -   h) HCDR1-3 comprising residues 31-35, 50-66, and 99-105 of SEQ         ID NO: 5, respectively, and LCDR1-3 comprising residues 24-34,         50-56, and 89-97 of SEQ ID NO: 6, respectively;     -   i) HCDR1-3 comprising residues 26-32, 52-57, and 99-105 of SEQ         ID NO: 5, respectively, and LCDR1-3 comprising residues 27-32,         50-52, and 89-97 of SEQ ID NO: 6, respectively; or     -   j) HCDR1-3 comprising residues 31-35, 52-57, and 99-105 of SEQ         ID NO: 5, respectively, and LCDR1-3 comprising residues 27-32,         50-52, and 89-97 of SEQ ID NO: 6, respectively.         In some embodiments, the antibody or fragment is humanized, or         chimeric with human constant regions. In further embodiments,         the antibody or fragment may comprise a human IgG₁, IgG₂, IgG₃,         or IgG₄ constant region and optionally a human κ constant         region.

In certain embodiments, the immunoconjugate of the invention comprises an anti-ROR1 antibody, or an antigen-binding fragment thereof, wherein the antibody comprises:

-   -   a) a heavy chain variable domain or region (V_(H)) comprising an         amino acid sequence at least 80%, 85%, 90%, 91%, 92%, 93%, 94%,         95%, 96%, 97%, 98%, or 99% (e.g., at least 90%) identical to         that of SEQ ID NO: 5, and a light chain variable domain or         region (V_(L)) comprising an amino acid sequence at least 80%,         85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% (e.g.,         at least 90%) identical to that of SEQ ID NO: 6;     -   b) a V_(H) and a V_(L) comprising the amino acid sequences of         SEQ ID NOs: 5 and 6, respectively;     -   c) a heavy chain (HC) comprising an amino acid sequence at least         80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99%         (e.g., at least 90%) identical to that of SEQ ID NO: 3 and a         light chain (LC) comprising an amino acid sequence 80%, 85%,         90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% (e.g., at         least 90%) identical to that of SEQ ID NO: 4; or     -   d) an HC and an LC comprising the amino acid sequences of SEQ ID         NOs: 3 and 4, respectively.

In certain embodiments, the V_(H) and V_(L) of the antibody respectively comprise the amino acid sequences of:

-   -   a) SEQ ID NOs: 5 and 50;     -   b) SEQ ID NOs: 48 and 6; or     -   c) SEQ ID NOs: 48 and 50.         In some embodiments, the antibody or fragment comprises a human         IgG₁, IgG₂, IgG₃, or IgG₄ constant region and optionally a human         κ constant region.

In certain embodiments, the HC and LC of the antibody respectively comprise the amino acid sequences of:

-   -   a) SEQ ID NOs: 3 and 49;     -   b) SEQ ID NOs: 47 and 4; or     -   c) SEQ ID NOs: 47 and 49.

In certain embodiments, the immunoconjugate of the invention comprises an antibody or fragment thereof derived from a murine antibody with the V_(H) and V_(L) amino acid sequences of (i) SEQ ID NOs: 25 and 26, respectively; (ii) SEQ ID NOs: 35 and 36, respectively; or (iii) SEQ ID NOs: 45 and 46, respectively. Antibodies derived from these sequences may be, e.g., antibodies that have been humanized or joined to a human Fc region (e.g., chimeric). For example, the antibody or an antigen-binding fragment in the immunoconjugate comprises:

-   -   a) a V_(H) comprising an amino acid sequence at least 80%, 85%,         90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% identical to         that of SEQ ID NO: 45 and a V_(L) comprising an amino acid         sequence at least 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%,         97%, 98%, or 99% identical to that of SEQ ID NO: 46;     -   b) a V_(H) comprising the amino acid sequence of SEQ ID NO: 45         and a V_(L) comprising the amino acid sequence of SEQ ID NO: 46;     -   c) a V_(H) comprising an amino acid sequence at least 80%, 85%,         90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% identical to         that of SEQ ID NO: 25 and a V_(L) comprising an amino acid         sequence at least 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%,         97%, 98%, or 99% identical to that of SEQ ID NO: 26; or     -   d) a V_(H) comprising the amino acid sequence of SEQ ID NO: 25         and a V_(L) comprising the amino acid sequence of SEQ ID NO: 26.

Exemplary coding sequences for the aforementioned antibodies are shown in Table 12 below. For example, the antibody in the immunoconjugate may comprise:

-   -   a) a V_(H) encoded by (i) nucleotides 73-420 of SEQ ID NO: 21,         or (ii) SEQ ID NO: 23; and a V_(L) encoded by SEQ ID NO: 22 or         24;     -   b) a V_(H) encoded by SEQ ID NO: 52 and a V_(L) encoded by SEQ         ID NO: 54;     -   c) a V_(H) encoded by SEQ ID NO: 33 and a V_(L) encoded by SEQ         ID NO: 34;     -   d) an HC encoded by nucleotides 73-1,410 of SEQ ID NO: 19 and an         LC encoded by nucleotides 73-714 of SEQ ID NO: 20; or     -   e) an HC encoded by SEQ ID NO: 51 and an LC encoded by         nucleotides SEQ ID NO: 53.

In certain embodiments, the immunoconjugate of the invention comprises an antigen-binding fragment of an anti-ROR1 antibody, wherein the antigen-binding fragment comprises the sequence of any one of SEQ ID NOs: 64-68. In certain embodiments, the antigen-binding fragment comprises the V_(H) and V_(L) amino acid sequences of:

-   -   a) SEQ ID NOs: 5 and 6;     -   b) SEQ ID NOs: 5 and 50;     -   c) SEQ ID NOs: 48 and 6;     -   d) SEQ ID NOs: 48 and 50;     -   e) SEQ ID NOs: 45 and 46; or     -   f) SEQ ID NOs: 25 and 26,         wherein the V_(H) amino acid sequence is optionally linked to         the amino acid sequence of SEQ ID NO: 62, and/or the V_(L) amino         acid sequence is optionally linked to the amino acid sequence of         SEQ ID NO: 63.

1.3 Antibody Sequence Comparison

Percent (%) sequence identity with respect to a reference polypeptide sequence refers to the percentage of amino acid residues in a candidate sequence that are identical with the amino acid residues in the reference sequence, after aligning the sequences and introducing gaps, if necessary, to achieve the maximum percent sequence identity. Alignment for purposes of determining percent amino acid sequence identity can be achieved in various ways that are known; for instance, using publicly available computer software such as BLAST, BLAST-2, ALIGN, ALIGN-2, or Megalign (DNASTAR). For purposes herein, however, % amino acid sequence identity values are generated using the sequence comparison computer program ALIGN-2. The ALIGN-2 sequence comparison computer program was authored by Genentech, Inc., and the source code has been filed with user documentation in the U.S. Copyright Office, Washington D.C., 20559, where it is registered under U.S. Copyright Registration No. TXU510087. The ALIGN-2 program is publicly available from Genentech, Inc., South San Francisco, Calif., or may be compiled from the source code. The ALIGN-2 program should be compiled for use on a UNIX operating system, including digital UNIX V4.0D. All sequence comparison parameters are set by the ALIGN-2 program and do not vary.

In situations where ALIGN-2 is employed for amino acid sequence comparison, the % amino acid sequence identity of a given amino acid sequence A to a given amino acid sequence B is calculated as follows: 100 times the fraction X/Y, where X is the number of amino acid residues scored as identical matches by the sequence alignment program ALIGN-2 in that program's alignment of A and B, and where Y is the total number of amino acid residues in B. It will be appreciated that where the length of amino acid sequence A is not equal to the length of amino acid sequence B, the % amino acid sequence identity of A to B will not equal the % amino acid sequence identity of B to A. Unless specifically stated otherwise, all % amino acid sequence identity values used herein are obtained as described in the immediately preceding paragraph using the ALIGN-2 computer program.

In some embodiments, amino acid sequence variants of the antibodies provided herein are contemplated. A variant typically differs from a polypeptide specifically disclosed herein in one or more substitutions, deletions, additions and/or insertions. Such variants can be naturally occurring or can be synthetically generated, for example, by modifying one or more of the above polypeptide sequences of the invention and evaluating one or more biological activities of the polypeptide as described herein and/or using any of a number of known techniques. For example, it may be desirable to improve the binding affinity and/or other biological properties of the antibody. Amino acid sequence variants of an antibody may be prepared by introducing appropriate modifications into the nucleotide sequence encoding the antibody, or by peptide synthesis. Such modifications include, for example, deletions from, and/or insertions into and/or substitutions of residues within the amino acid sequences of the antibody. Any combination of deletion, insertion, and substitution can be made to arrive at the final construct, provided that the final construct possesses the desired characteristics, e.g., antigen-binding.

As used herein, the term “substantially identical” refers to two or more sequences having a percentage of sequential units (e.g., amino acid residues) which are the same when compared and aligned for maximum correspondence over a comparison window, or a designated region as measured using comparison algorithms. By way of example, two or more sequences may be “substantially identical” if the sequential units are about 60% identical, about 65% identical, about 70% identical, about 75% identical, about 80% identical, about 85% identical, about 90% identical, about 95% identical, about 96% identical, about 97% identical, about 98% identical, or about 99% identical over a specified region. Such percentages describe the “percent identity” between two sequences.

1.4 Making and Modification of ROR1 Antibodies

Anti-ROR1 antibodies for use in the immunoconjugates of the present invention can be made by immunizing an animal with human ROR1 or a fragment of human ROR1 protein. Antibodies that bind to the immunizing fragment with high affinity (e.g., with a K_(D) in the nM or lower range) can be screened by using routine methods such as ELISA.

If the antibody is a non-human antibody, it can be humanized. A “humanized” antibody is an antibody in which all or substantially all CDR amino acid residues are derived from a non-human (e.g., mouse or rat) antibody and all or substantially all FR amino acid residues are derived from human FRs. A humanized antibody optionally may include at least a portion of a constant region derived from a human antibody. A “humanized form” of a non-human antibody refers to a variant of the non-human antibody that has undergone humanization, typically to reduce immunogenicity to humans, while retaining the antigen-binding specificity and affinity of the parental non-human antibody. In some embodiments, some FR residues in a humanized antibody are substituted with corresponding residues from the cognate non-human antibody to restore or improve the resultant antibody's antigen-binding specificity and/or affinity.

ROR1 antibodies or fragments may be manufactured recombinantly in mammalian host cells containing coding sequences for the ROR1 antibodies or fragments, wherein the coding sequences are operably linked to transcription-regulatory elements suitable for expression in the host cells. The coding sequences may be introduced into the host cells on one or more vectors. Useful mammalian host cells include, inter alia, Chinese hamster ovary (CHO) cells, NSO cells, SP2 cells, HEK-293T cells, 293 Freestyle cells (Invitrogen), NIH-3T3 cells, HeLa cells, baby hamster kidney (BHK) cells, African green monkey kidney cells (COS), human hepatocellular carcinoma cells (e.g., Hep G2), and A549 cells. Cell lines may be selected based on their expression levels. Other cell lines that may be used include insect cell lines, such as Sf9 or Sf21 cells, and yeast cell lines.

In some embodiments, a parent ROR1 antibody may be engineered by introducing one or more amino acid substitutions to improve the antibody's antigen binding, to decrease immunogenicity (e.g., de-immunize; see, e.g., Jones et al., Methods Mol Biol. 525:405-23 (2009)), and/or to improve antibody-dependent cell-mediated cytotoxicity (ADCC) or complement-dependent cytotoxicity (CDC).

In some embodiments, substitutions, insertions, or deletions may be made within one or more CDRs, wherein the mutations do not substantially reduce the antibody's binding to its antigen. For example, conservative substitutions that do not substantially reduce binding affinity may be made.

Alterations (e.g., substitutions) may be made in CDRs to improve antibody affinity. CDR residues involved in antigen binding may be identified by using, e.g., alanine scanning mutagenesis or computer modeling. HCDR3 and LCDR3 in particular are often targeted. A crystal structure of an antigen-antibody complex may also be used to identify contact points between the antibody and its antigen. Such contact residues and their neighboring residues may be targeted for mutations. Variants may be screened to determine whether they obtain the desired properties. In vitro affinity maturation (e.g., using error-prone PCR, chain shuffling, randomization of CDRs, or oligonucleotide-directed mutagenesis) may also be used to improve antibody affinity (see, e.g., Hoogenboom et al., Methods in Molecular Biology 178:1-37 (2001)).

Amino acid sequence insertions and deletions made to an antibody or antibody fragment include amino- and/or carboxyl-terminal fusions ranging in length from one or a few residues to polypeptides containing a hundred or more residues, as well as intra-sequence insertions and deletions of single or multiple amino acid residues. Examples of terminal insertions include an antibody with an N-terminal methionyl residue. Other insertional variants of the antibody molecule include the fusion of the N- or C-terminus of the antibody to an enzyme (e.g., for ADEPT) or a polypeptide that increases the serum half-life of the antibody. Examples of intra-sequence insertion variants of the antibody molecules include an insertion of 3 amino acids in the light chain. Examples of terminal deletions include an antibody with a deletion of 7 or fewer amino acids at an end of the light chain, and the removal of the C-terminal lysine in the heavy chain.

In some embodiments, the ROR1 antibodies are altered to increase or decrease their glycosylation (e.g., by altering the amino acid sequence such that one or more glycosylation sites are created or removed). A carbohydrate attached to an Fc region of an antibody may be altered. Native antibodies from mammalian cells typically comprise a branched, biantennary oligosaccharide attached by an N-linkage to Asn₂₉₇ of the CH₂ domain of the Fc region (see, e.g., Wright et al., TIBTECH 15:26-32 (1997)). Asn₂₉₇ refers to the asparagine residue located at about position 297 in the Fc region (EU numbering of Fc region residues; see, e.g., Edelman et al. PNAS 63(1):78-85 (1969)). However, Asn₂₉₇ may also be located about ±3 amino acids upstream or downstream of position 297, i.e., between positions 294 and 300, due to minor sequence variations in antibodies. The oligosaccharide can be any of various carbohydrates, e.g., mannose, N-acetyl glucosamine (GlcNAc), galactose, sialic acid, or fucose attached to a GlcNAc in the stem of the biantennar oligosaccharide structure. Modifications of the oligosaccharide in an antibody can be made, for example, to create antibody variants with certain improved properties. Antibody glycosylation variants can have improved ADCC and/or CDC function.

In some embodiments, antibody variants are provided having a carbohydrate structure that has no or a reduced level of fucose attached (directly or indirectly) to an Fc region. For example, the amount of fucose in such an antibody may be from 1% to 80%, from 1% to 65%, from 5% to 65% or from 20% to 40%. The amount of fucose is determined by calculating the average amount of fucose within the sugar chain at Asn₂₉₇, relative to the sum of all glycostructures attached to Asn₂₉₇ (see, e.g., PCT Patent Publication WO 2008/077546). Such fucosylation variants can have improved ADCC function (see, e.g., Okazaki et al., J. Mol. Biol. 336:1239-1249 (2004); and Yamane-Ohnuki et al., Biotech. Bioeng. 87:614 (2004)). Cell lines (e.g., knockout cell lines) can be used to produce defucosylated antibodies, e.g., Lec13 CHO cells deficient in protein fucosylation and alpha-1,6-fucosyltransferase gene (FUT8) knockout CHO cells (see, e.g., Ripka et al., Arch. Biochem. Biophys. 249:533-545 (1986); Yamane-Ohnuki et al., Biotech. Bioeng. 87:614 (2004); and Kanda et al., Biotechnol. Bioeng. 94(4):680-688 (2006)). Other antibody glycosylation variants as described in, e.g., U.S. Pat. No. 6,602,684) may also be made to the ROR1 antibodies or antibody fragments for use in the present immunoconjugates.

In some embodiments, one or more amino acid modifications may be introduced into the Fc region of a ROR1 antibody to generate a ROR1 antibody with a variant Fc region that confers new properties to the antibody. A variant Fc region may comprise a human Fc region sequence (e.g., a human IgG₁, IgG₂, IgG₃ or IgG₄ Fc region) comprising an amino acid modification (e.g., a substitution) at one or more amino acid positions. For example, a ROR1 antibody with a variant Fc region may possess some but not all effector functions, which makes it a desirable candidate for applications in which the half-life of the antibody in vivo is important yet certain effector functions (such as complement and ADCC) are unnecessary or deleterious. In vitro and/or in vivo cytotoxicity assays can be conducted to confirm the reduction/depletion of CDC and/or ADCC activities. For example, Fc receptor (FcR) binding assays can be conducted to ensure that the antibody lacks FcγR binding (hence likely lacking ADCC activity), but retains FcRn binding ability. Non-limiting examples of in vitro assays to assess ADCC activity of a molecule of interest are described in U.S. Pat. Nos. 5,500,362 and 5,821,337. Alternatively, non-radioactive assays methods may be employed (e.g., ACTI™ and CytoTox 96® non-radioactive cytotoxicity assays). Useful effector cells for such assays include peripheral blood mononuclear cells (PBMCs), monocytes, macrophages, and natural killer (NK) cells.

Antibodies can have increased half-lives and improved binding to the neonatal Fc receptor (FcRn) (see, e.g., U.S. Patent Publication 2005/0014934). Such antibodies can comprise an Fc region with one or more substitutions therein which improve binding of the Fc region to FcRn, and include those with substitutions at one or more of Fc region residues: 238, 256, 265, 272, 286, 303, 305, 307, 311, 312, 317, 340, 356, 360, 362, 376, 378, 380, 382, 413, 424 and 434 according to the EU numbering system (see, e.g., U.S. Pat. No. 7,371,826). Other examples of Fc region variants are also contemplated (see, e.g., Duncan & Winter, Nature 322:738-40 (1988); U.S. Pat. Nos. 5,648,260 and 5,624,821; and PCT Publication WO 94/29351).

In some embodiments, it may be desirable to create cysteine engineered antibodies, e.g., “thioMAbs,” in which one or more residues of an antibody are substituted with cysteine residues. In some embodiments, the substituted residues occur at accessible sites of the antibody. Reactive thiol groups can be positioned at sites for conjugation to other moieties, such as drug moieties or linker drug moieties, to create an immunoconjugate. Any one or more of the following residues may be substituted with cysteine: V205 (Kabat numbering) of the light chain; Al 18 (EU numbering) of the heavy chain; and S400 (EU numbering) of the heavy chain Fc region.

An antibody provided herein may be further modified to include non-proteinaceous moieties. The moieties suitable for derivatization of the antibody include but are not limited to water soluble polymers. The term “polymer,” as used herein, refers to a molecule composed of repeated subunits; such molecules include, but are not limited to, polypeptides, polynucleotides, or polysaccharides, or polyalkylene glycols. Non-limiting examples of water soluble polymers are polyethylene glycol (PEG), copolymers of ethylene glycol/propylene glycol, carboxymethylcellulose, dextran, polyvinyl alcohol, polyvinyl pyrrolidone, poly-1,3-dioxolane, poly-1,3,6-trioxane, ethylene/maleic anhydride copolymer, polyamino acids (either homopolymers or random copolymers), and dextran or poly(N-vinyl pyrrolidone)-polyethylene glycol, polypropylene glycol homopolymers, polypropylene oxide/ethylene oxide co-polymers, polyoxyethylated polyols (e.g., glycerol), polyvinyl alcohol, and mixtures thereof. Polyethylene glycol propionaldehyde may have advantages in manufacturing due to its stability in water. The polymer may be of any molecular weight, and may be branched or unbranched. The number of polymers attached to the antibody may vary, and if two or more polymers are attached, they can be the same or different molecules.

1.5 Cytotoxic Drug Moieties

An immunoconjugate of the invention comprises an anti-ROR1 antibody or an antigen-binding fragment thereof conjugated to one or more cytotoxic agents, such as chemotherapeutic agents, growth inhibitory agents, toxins (e.g., protein toxins, enzymatically active toxins of bacterial, fungal, plant, or animal origin, or fragments thereof), or radioactive isotopes. The cytotoxic agent(s) can be conjugated to the anti-ROR1 antibody or fragment by a linker covalently bound to an amino acid residue of the antibody. Many drugs that can serve as a cytotoxic moiety in an immunoconjugate are independently too toxic to be used for cancer treatment, and thus are more effective when specifically targeted to the cancer cell by an antibody or antibody fragment.

The term “cytotoxic drug moiety” or “cytotoxic agent” refers to a compound that can cause harm, disturbances, or death to a cell. Examples of cytotoxic drug moieties that can be used as part of a ROR1 immunoconjugate include, but are not limited to: NCA1, auristatin, auristatin E, DNA minor groove binding agents, DNA minor groove alkylating agents, enediyne, lexitropsin, duocarmycin, taxane, puromycin, dolastatin, maytansinoid, vinca alkaloid, AFP, MMAF, MMAE, AEB, AEVB, taxoids (e.g., paclitaxel and paclitaxel derivatives (TAXOL®, Bristol-Myers Squibb Oncology, Princeton, N.J.), ABRAXANE® (American Pharmaceutical Partners, Schaumberg, Ill.), as well as docetaxel and docetaxel derivatives), CC-1065, SN-38, topotecan, morpholino-doxorubicin, rhizoxin, cyanomorpholino-doxorubicin, dolastatin-10, echinomycin, combretatstatin, chalicheamicin, maytansine, DM-1, netropsin, podophyllotoxin (e.g., etoposide and teniposide), baccatin and its derivatives, anti-tubulin agents, cryptophysin, combretastatin, vincristine, vincristine sulfate, vinblastine, vindesine, vinorelbine, VP-16, camptothecin, epothilone A, epothilone B, nocodazole, colchicines, colcimid, estramustine, cemadotin, discodermolide, eleutherobin, mechlorethamine, cyclophosphamide, melphalan, carmustine, lomustine, semustine, streptozocin, chlorozotocin, uracil mustard, chlormethine, chlorambucil, pipobroman, triethylenemelamine, triethylenethiophosphoramine, busulfan, dacarbazine, temozolomide, ytarabine, cytosine arabinoside, fluorouracil, 5-fluorouracil (5-FU), floxuridine, 6-thioguanine, 6-mercaptopurine, pentostatin, methotrexate, 10-propargyl-5,8-dideazafolate, 5,8-dideazatetrahydrofolic acid, leucovorin, fludarabine phosphate, pentostatine, gemcitabine, Ara-C, deoxycoformycin, mitomycins such as mitomycin-C, L-asparaginase, azathioprine, brequinar, antibiotics (e.g., anthracycline, gentamicin, cefalotin, vancomycin, telavancin, daptomycin, azithromycin, erythromycin, rocithromycin, furazolidone, amoxicillin, ampicillin, carbenicillin, flucloxacillin, methicillin, penicillin, ciprofloxacin, moxifloxacin, ofloxacin, doxycycline, minocycline, oxytetracycline, tetracycline, streptomycin, rifabutin, ethambutol, and rifaximin), enediyne antibiotics (e.g., calicheamicin, calicheamicin gammalI and calicheamicin omegaIl, and dynemicin, including dynemicin A), antiviral drugs (e.g., abacavir, acyclovir, ampligen, cidofovir, delavirdine, didanosine, efavirenz, entecavir, fosfonet, ganciclovir, ibacitabine, immunovir, idoxuridine, inosine, lopinavir, methisazone, nexavir, nevirapine, oseltamivir, penciclovir, stavudine, trifluridine, truvada, valaciclovir, and zanamivir), daunorubicin hydrochloride, daunoriycin, rubidomycin, cerubidine, idarubicin, doxorubicin, epirubicin and morpholino derivatives, phenoxizone biscyclopeptides (e.g., dactinomycin), basic glycopeptides (e.g., bleomycin), anthraquinone glycosides (e.g., plicamycin and mithramycin), anthracenediones (e.g., mitoxantrone), azirinopyrrolo indolediones (e.g., mitomycin), macrocyclic immunosuppressants (e.g., cyclosporine, FK-506, tacrolimus, prograf, and rapamycin), navelbene, CPT-11, anastrazole, letrazole, capecitabine, reloxafine, droloxafine, allocolchicine, Halichondrin B, colchicine and colchicine derivatives, rhizoxin, thiocolchicine, trityl cysterin, vinblastine sulfate, hydroxyurea, N-methylhydrazine, epidophyllotoxin, procarbazine, mitoxantrone, leucovorin, and tegafur. “Taxanes” include paclitaxel, as well as any active taxane derivative or pro-drug. Chemotherapeutic agents such as erlotinib (TARCEVA®, Genentech/OSI Pharm.), bortezomib (VELCADE®, Millenium Pharm.), fulvestrant (FASLODEX®, AstraZeneca), sunitinib (Sutent®, Pfizer), letrozole (FEMARA®, Novartis), imatinib mesylate (GLEEVEC®, Novartis), PTK787/ZK 222584 (Novartis), oxaliplatin (Eloxatin®, Sanofi), leucovorin, lapatinib (TYKERB®, GSK572016, GlaxoSmithKline), lonafarnib (SCH 66336), sorafenib (BAY43-9006, Bayer Labs.), and gefitinib (IRESSA®, AstraZeneca), AG1478, AG1571 (SU 5271; Sugen), alkylating agents such as thiotepa and cyclosphosphamide (CYTOXAN®); alkyl sulfonates such as busulfan, improsulfan and piposulfan; antifolate antineoplastic such as pemetrexed (ALIMTA® Eli Lilly); aziridines such as benzodopa, carboquone, meturedopa, and uredopa; ethylenimines and methylamelamines including altretamine, triethylenemelamine, triethylenephosphoramide, triethylenethiophosphoramide and trimethylomelamine; acetogenins (such as bullatacin and bullatacinone); a camptothecin (including the synthetic analogue topotecan); bryostatin; callystatin; CC-1065 (including its synthetic analogues adozelesin, carzelesin and bizelesin); cryptophycins (such as cryptophycin 1 and cryptophycin 8); dolastatin; duocarmycin (including its synthetic analogues KW-2189 and CB1-TM1); eleutherobin; pancratistatin; a sarcodictyin; spongistatin; nitrogen mustards such as chlorambucil, chlornaphazine, cholophosphamide, estramustine, ifosfamide, mechlorethamine, mechlorethamine oxide hydrochloride, melphalan, novembichin, phenesterine, prednimustine, trofosfamide, and uracil mustard; nitrosoureas such as carmustine, chlorozotocin, fotemustine, lomustine, nimustine, and ranimnustine; bisphosphonates, such as clodronate; an esperamicin; as well as neocarzinostatin chromophore and related chromoprotein enediyne antibiotic chromophores, aclacinomysins, actinomycin, anthramycin, azaserine, bleomycins, cactinomycin, carabicin, caminomycin, carzinophilin, chromomycinis, dactinomycin, daunorubicin, detorubicin, 6-diazo-5-oxo-L-norleucine, doxorubicin (ADRIAMYCIN®) (including morpholino-doxorubicin, cyanomorpholino-doxorubicin, 2-pyrrolino-doxorubicin and deoxydoxorubicin), epirubicin, esorubicin, idarubicin, marcellomycin, mycophenolic acid, nogalamycin, olivomycins, peplomycin, potfiromycin, puromycin, quelamycin, rodorubicin, streptonigrin, streptozocin, tubercidin, ubenimex, zinostatin, zorubicin; anti-metabolites such as methotrexate and 5-FU; folic acid analogues such as denopterin, methotrexate, pteropterin, trimetrexate; purine analogs such as fludarabine, 6-mercaptopurine, thiamiprine, thioguanine; pyrimidine analogs such as ancitabine, azacitidine, 6-azauridine, carmofur, cytarabine, dideoxyuridine, doxifluridine, enocitabine, floxuridine; androgens such as calusterone, dromostanolone propionate, epitiostanol, mepitiostane, testolactone; anti-adrenals such as aminoglutethimide, mitotane, trilostane; folic acid replenisher such as frolinic acid; aceglatone; aldophosphamide glycoside; aminolevulinic acid; eniluracil; amsacrine; bestrabucil; bisantrene; edatraxate; defofamine; demecolcine; diaziquone; elformithine; elliptinium acetate; an epothilone; etoglucid; gallium nitrate; hydroxyurea; lentinan; lonidainine; maytansinoids such as maytansine and ansamitocins; mitoguazone; mitoxantrone; mopidanmol; nitraerine; pentostatin; phenamet; pirarubicin; losoxantrone; podophyllinic acid; 2-ethylhydrazide; procarbazine; PSK® polysaccharide complex (JHS Natural Products, Eugene, Oreg.); razoxane; rhizoxin; sizofuran; spirogermanium; tenuazonic acid; triaziquone; 2,2′,2″-trichlorotriethylamine; trichothecenes (especially T-2 toxin, verracurin A, roridin A and anguidine); urethan; vindesine; dacarbazine; mannomustine; mitobronitol; mitolactol; pipobroman; gacytosine; arabinoside (“Ara-C”); cyclophosphamide; thiotepa; taxoids, e.g., paclitaxel (TAXOL®, Bristol-Myers Squibb Oncology, Princeton, N.J.), ABRAXANE™ Cremophor-free, albumin, nanoparticle formulation of paclitaxel (American Pharmaceutical Partners, Schaumberg, Ill.), and TAXOTERE® doxetaxel (Rhone-Poulenc Rorer, Antony, France); chloranbucil; GEMZAR® gemcitabine; 6-thioguanine; mercaptopurine; methotrexate; platinum analogs such as cisplatin and carboplatin; vinblastine; platinum; etoposide (VP-16); mitoxantrone; NAVELBINE® vinorelbine; novantrone; teniposide; edatrexate; daunomycin; aminopterin; xeloda; ibandronate; topoisomerase inhibitor RFS 2000; difluoromethylornithine (DMFO); retinoids such as retinoic acid; and pharmaceutically acceptable salts, esters, acids, prodrugs, or derivatives of any of the above.

In some embodiments, a suitable cytotoxic agent for use in the ROR1 immunoconjugate is

-   -   a) an anti-tubulin agent (e.g., an auristatin or dolastatin such         as auristatin E, monomethyl auristatin E (MMAE) or monomethyl         auristatin F (MMAF),         dimethylvaline-valine-dolaisoleuine-dolaproine-phenylalanine-p-phenylenediamine         (AFP), 5-benzoylvaleric acid-auristatin E ester (AEVB), AEB, a         maytansinoid, ansamitocin, mertansine/emtansine (DM1), or         ravtansine/soravtansine (DM4)),     -   b) a DNA alkylating agent or a DNA minor groove alkylating agent         (e.g., duocarmycin),     -   c) a DNA cross-linking agent (e.g., pyrrolobenzodiazepine         (PBD)),     -   d) a DNA intercalating agent (e.g., PNU-159682),     -   e) a DNA minor groove binding agent (e.g., CC-1065), or     -   f) an RNA polymerase II inhibitor (e.g., amanitin, such as         α-amanitin).         As used herein, the term “intercalating agent” refers to a         chemical that can insert into the intramolecular space of a         molecule or the intermolecular space between molecules. By way         of example, a DNA intercalating agent may be a molecule that         inserts into the stacked bases of the DNA double helix.

In some embodiments, the cytotoxic agent used in the ROR1 immunoconjugate is selected from the group consisting of an enediyne, a lexitropsin, a duocarmycin, a taxane, a puromycin, a podophyllotoxin, a baccatin derivative, a cryptophysin, a combrestatin, a dolastatin, a maytansinoid, a vinca alkaloid, paclitaxel, docetaxel, ansamitocin, CC-1065, SN-38, topotecan, morpholino-doxorubicin, rhizoxin, cyanomorpholino-doxorubicin, dolastatin-10, echinomycin, combretatstatin, chalicheamicin, vincristine, vinblastine, vindesine, vinorelbine, VP-16, camptothecin, epothilone A, epothilone B, nocodazole, colchicines, colcimid, estramustine, cemadotin, discodermolide maytansine, eleutherobin, and netropsin.

In some embodiments, the cytotoxic drug moiety in the immunoconjugate is a prodrug. The term “prodrug” or “pharmaceutically acceptable prodrug,” as used herein, refers to an agent that is converted into the parent drug in vivo or in vitro and is relatively nontoxic. That is, the immunoconjugate may be administered to a subject without causing undesirable biological effects or interacting in a deleterious manner with any of the components of the composition in which it is contained. Prodrugs are generally drug precursors that, following administration to a subject and subsequent absorption, are converted to an active or a more active species via some process, such as conversion by a metabolic pathway. Some prodrugs have a chemical group present on the prodrug that renders it less active and/or confers solubility or some other property to the drug. Once the chemical group has been cleaved and/or modified from the prodrug, the active drug is generated. Prodrugs may be converted into active drug within the body through enzymatic or non-enzymatic reactions. Prodrugs may provide improved physiochemical properties over their parent drugs such as better solubility, enhanced delivery characteristics (e.g., targeting a particular cell, tissue, organ or ligand), and improved therapeutic value of the drug. The benefits of such prodrugs may include, but are not limited to, (i) ease of administration compared with the parent drug; (ii) the prodrug may be bioavailable by oral administration where the parent is not; and (iii) the prodrug may have improved solubility in pharmaceutical compositions compared with the parent drug. A prodrug includes a pharmacologically inactive or less active derivative of a drug. Prodrugs may be designed to modulate the amount of a drug or biologically active molecule that reaches a desired site of action through the manipulation of the properties of a drug, such as physiochemical, biopharmaceutical, or pharmacokinetic properties.

In some embodiments, the average number of the drug moiety to the antibody in the immunoconjugate (i.e., drug-to-antibody ratio or DAR) is 1; or at least 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4, 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, 5, 5.1, 5.2, 5.3, 5.4, 5.5, 6, 7, 8, 9, or 10. Exemplary methods for measuring DAR are described in the Examples below.

1.6 Linkers

In certain embodiments of an immunoconjugate of the invention, the antibody can be conjugated directly to the cytotoxic agent, or can be conjugated via a linker. Suitable linkers include, for example, cleavable and non-cleavable linkers. In some embodiments, the linker is a cleavable linker. A cleavable linker refers to a linker that comprises a cleavable moiety and is typically susceptible to cleavage under intracellular conditions. Suitable cleavable linkers include, for example, peptide linkers cleavable by an intracellular protease (such as a lysosomal protease or an endosomal protease), and acid-cleavable linkers. In exemplary embodiments, the linker can be a dipeptide, such as a valine-citrulline (val-cit or VC) or a phenylalanine-lysine (phe-lys) linker. The linker may also be a tripeptide or a polypeptide having four or more amino acid residues. Other suitable linkers include linkers hydrolyzable at a pH of less than 5.5, such as a hydrazone linker. Additional suitable cleavable linkers include disulfide linkers. In some embodiments, the linker is a non-polymeric linker. In some cases, the linker is a non-peptide linker or a linker that does not contain an amino acid residue.

In some embodiments, the linker includes a C₁-C₆ alkyl group (e.g., a C₅, C₄, C₃, C₂, or C₁ alkyl group). As used herein the term “alkyl” refers to a straight or branched hydrocarbon chain radical consisting solely of carbon and hydrogen atoms, containing no unsaturation. C₁-C_(x) includes C₁-C₂, C₁-C₃, . . . , C₁-C_(x), where x is an integer. C₁-C_(x) refers to the number of carbon atoms in the designated group. In some embodiments, an alkyl comprises one to eight carbon atoms (C₁₋₈ alkyl). In some embodiments, an alkyl comprises two to six carbon atoms (C₂₋₆ alkyl).

As used herein, a linker prior to the chemical reaction to link the Ab (antibody or fragment) and D (payload) components of the immunoconjugate is also called a “linker precursor.” It will be apparent to the skilled person in the ADC art whether a certain chemical entity disclosed herein is a linker precursor based on its reactive capabilities, or a linker component in the final immunoconjugate product.

In some embodiments, the linkage between the Ab and D components of the immunoconjugate may be formed through reaction of the components with a homobifuctional linker. Exemplary homobifuctional linkers include, but are not limited to, Lomant's reagent dithiobis (succinimidylpropionate) DSP, 3′3′-dithiobis(sulfosuccinimidyl propionate) (DTSSP), disuccinimidyl suberate (DSS), bis(sulfosuccinimidyl)suberate (BS), disuccinimidyl tartrate (DST), disulfosuccinimidyl tartrate (sulfo DST), ethylene glycobis(succinimidylsuccinate) (EGS), disuccinimidyl glutarate (DSG), N,N′-disuccinimidyl carbonate (DSC), dimethyl adipimidate (DMA), dimethyl pimelimidate (DMP), dimethyl suberimidate (DMS), dimethyl-3,3′-dithiobispropionimidate (DTBP), 1,4-di-3′-(2′-pyridyldithio)propionamido)butane (DPDPB), bismaleimidohexane (BMH), aryl halide-containing compound (DFDNB), such as, e.g. 1,5-difluoro-2,4-dinitrobenzene or 1,3-difluoro-4,6-dinitrobenzene, 4,4′-difluoro-3,3′-dinitrophenylsulfone (DFDNPS), bis-[β-(4-azidosalicylamido)ethyl]disulfide (BASED), formaldehyde, glutaraldehyde, 1,4-butanediol diglycidyl ether, adipic acid dihydrazide, carbohydrazide, o-toluidine, 3,3′-dimethylbenzidine, benzidine, α,α′-p-diaminodiphenyl, diiodo-p-xylene sulfonic acid, N,N′-ethylene-bis(iodoacetamide), and N,N′-hexamethylene-bis(iodoacetamide).

In some embodiments, the linkage between the Ab and D components of the immunoconjugate may be formed through reaction of the components with a heterobifunctional linker. Exemplary heterobifunctional linkers include, but are not limited to, amine-reactive and sulfhydryl cross-linkers such as N-succinimidyl 3-(2-pyridyldithio)propionate (sPDP), long-chain N-succinimidyl 3-(2-pyridyldithio)propionate (LC-sPDP), water-soluble-long-chain N-succinimidyl 3-(2-pyridyldithio) propionate (sulfo-LC-sPDP), succinimidyloxycarbonyl-a-methyl-α-(2-pyridyldithio)toluene (sMPT), sulfosuccinimidyl-6-[α-methyl-α-(2-pyridyldithio)toluamido]hexanoate (sulfo-LC-sMPT), succinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate (SMCC), sulfosuccinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate (sulfo-sMCC), m-maleimidobenzoyl-N-hydroxysuccinimide ester (MBs), m-maleimidobenzoyl-N-hydroxysulfosuccinimide ester (sulfo-MBs), N-succinimidyl(4-iodoacteyl)aminobenzoate (sIAB), sulfosuccinimidyl(4-iodoacteyl)aminobenzoate (sulfo-sIAB), succinimidyl-4-(p-maleimidophenyl)butyrate (sMPB), sulfosuccinimidyl-4-(p-maleimidophenyl)butyrate (sulfo-sMPB), N-(γ-maleimidobutyryloxy)succinimide ester (GMBs), N-(γ-maleimidobutyryloxy)sulfosuccinimide ester (sulfo-GMBs), succinimidyl 6-((iodoacetyl)amino)hexanoate (sIAX), succinimidyl 6-[6-(((iodoacetyl)amino)hexanoyl)amino]hexanoate (sIAXX), succinimidyl 4-(((iodoacetyl)amino)methyl)cyclohexane-1-carboxylate (sIAC), succinimidyl 6-((((4-iodoacetyl)amino)methyl)cyclohexane-1-carbonyl)amino) hexanoate (sIACX), p-nitrophenyl iodoacetate (NPIA), carbonyl-reactive and sulfhydryl-reactive cross-linkers such as 4-(4-N-maleimidophenyl)butyric acid hydrazide (MPBH), 4-(N-maleimidomethyl)cyclohexane-1-carboxyl-hydrazide-8 (M₂C₂H), 3-(2-pyridyldithio)propionyl hydrazide (PDPH), amine-reactive and photoreactive cross-linkers such as N-hydroxysuccinimidyl-4-azidosalicylic acid (NHs-AsA), N-hydroxysulfosuccinimidyl-4-azidosalicylic acid (sulfo-NHs-AsA), sulfosuccinimidyl-(4-azidosalicylamido)hexanoate (sulfo-NHs-LC-AsA), sulfosuccinimidyl-2-(p-azidosalicylamido)ethyl-1,3′-dithiopropionate (sAsD), N-hydroxysuccinimidyl-4-azidobenzoate (HsAB), N-hydroxysulfosuccinimidyl-4-azidobenzoate (sulfo-HsAB), N-succinimidyl-6-(4′-azido-2′-nitrophenylamino)hexanoate (sANPAH), sulfosuccinimidyl-6-(4′-azido-2′-nitrophenylamino)hexanoate (sulfo-sANPAH), N-5-azido-2-nitrobenzoyloxysuccinimide (ANB-NOs), sulfosuccinimidyl-2-(m-azido-o-nitrobenzamido)-ethyl-1,3′-dithiopropionate (sAND), N-succinimidyl-4(4-azidophenyl)1,3′-dithiopropionate (sADP), N-sulfosuccinimidyl(4-azidophenyl)-1,3′-dithiopropionate (sulfo-sADP), sulfosuccinimidyl 4-(p-azidophenyl)butyrate (sulfo-sAPB), sulfosuccinimidyl 2-(7-azido-4-methylcoumarin-3-acetamide)ethyl-1,3′-dithiopropionate (sAED), sulfosuccinimidyl 7-azido-4-methylcoumain-3-acetate (sulfo-sAMCA), p-nitrophenyl diazopyruvate (pNPDP), p-nitrophenyl-2-diazo-3,3,3-trifluoropropionate (PNP-DTP), sulfhydryl-reactive and photoreactive cross-linkers such as 1-(p-Azidosalicylamido)-4-(iodoacetamido)butane (AsIB), N-[4-(p-azidosalicylamido)butyl]-3′-(2′-pyridyldithio)propionamide (APDP), benzophenone-4-iodoacetamide, benzophenone-4-maleimide carbonyl-reactive and photoreactive cross-linkers such as p-azidobenzoyl hydrazide (ABH), carboxylate-reactive and photoreactive cross-linkers such as 4-(p-azidosalicylamido)butylamine (AsBA), and arginine-reactive and photoreactive cross-linkers such as p-azidophenyl glyoxal (APG).

In some embodiments, the linkage between the Ab and D components of the immunoconjugate may be formed through reaction of the components with a linker having a reactive functional group that may comprise, e.g., a nucleophilic group that is reactive to an electrophilic group present on a binding moiety. Exemplary electrophilic groups include carbonyl groups such as aldehydes, ketones, carboxylic acids, esters, amides, enones, acyl halides, and acid anhydrides. In particular embodiments, the reactive functional group is an aldehyde. Exemplary nucleophilic groups include hydrazide, oxime, amino, hydrazine, thiosemicarbazone, hydrazine carboxylate, and arylhydrazide.

In some embodiments, the conjugation of the linker/payload to the antibody or fragment may be formed through reaction with a maleimide group (which may also be referred to as a maleimide spacer). In certain embodiments, the maleimide group is maleimidocaproyl (mc); thus, the linker/payload is conjugated to the antibody or fragment through reaction between a residue on the antibody or fragment and the me group in the linker precursor. In some embodiments, the maleimide group comprises a maleimidomethyl group, such as succinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate (SMCC) or sulfosuccinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate (sulfo-SMCC), as described herein.

In some embodiments, the maleimide group is a self-stabilizing maleimide. In some embodiments, the self-stabilizing maleimide utilizes diaminopropionic acid (DPR) to incorporate a basic amino group adjacent to the maleimide to provide intramolecular catalysis of thiosuccinimide ring hydrolysis, thereby decreasing the ability of the maleimide to undergo an elimination reaction through a retro-Michael reaction. In some embodiments, the self-stabilizing maleimide is a maleimide group described in Lyon et al., Nat. Biotechnol. 32(10):1059-1062 (2014). In certain embodiments, the linker precursor comprises a self-stabilizing maleimide. In certain embodiments, the linker precursor is a self-stabilizing maleimide.

In some embodiments, the linker may include a peptide moiety. In some embodiments, the peptide moiety comprises at least 2, 3, 4, 5, 6, 7, 8, or more amino acid residues. In some embodiments, the peptide moiety is cleavable (e.g., either enzymatically or chemically). In some embodiments, the peptide moiety is non-cleavable. In some embodiments, the peptide moiety comprises Val-Cit (valine-citrulline), Gly-Gly-Phe-Gly (SEQ ID NO: 55), Phe-Lys, Val-Lys, Gly-Phe-Lys, Phe-Phe-Lys, Ala-Lys, Val-Arg, Phe-Cit, Phe-Arg, Leu-Cit, Ile-Cit, Trp-Cit, Phe-Ala, Ala-Leu-Ala-Leu (SEQ ID NO: 56), or Gly-Phe-Leu-Gly (SEQ ID NO: 57). In certain embodiments, the linker comprises Val-Cit (VC). In certain embodiments, the linker is Val-Cit (VC).

In some embodiments, the linker may include a benzoic acid or benzyloxy group, or a derivative thereof. For example, the linker may comprise para-amino-benzoic acid (PABA). In some embodiments, the linker includes a para-amino-benzyloxycarbonyl (PAB) group. In some embodiments, the linker comprises gamma-amino-butyric acid (GABA).

In some embodiments, the linkage between the Ab and D components of the immunoconjugate may be formed through reaction of the components with a linker comprising a maleimide group, a peptide moiety, and/or a benzoic acid (e.g., PABA) or benzyloxycarbonyl group, in any combination. In certain embodiments, the maleimide group is maleimidocaproyl (mc). In certain embodiments, the peptide group is Val-Cit (VC). In certain embodiments, the linker comprises a Val-Cit-PABA group. In certain embodiments, the conjugation of the linker to the antibody or fragment may be formed from an mc-Val-Cit-PABA group. In certain embodiments, the conjugation of the linker to the antibody or fragment may be formed from an mc-Val-Cit group. In certain embodiments, the linkage between the antibody or fragment and the drug moiety may be formed from an mc-Val-Cit-PAB group.

In some embodiments, the linker is a self-immolative linker or a self-elimination linker (e.g., a cyclization self-elimination linker). In some embodiments, the linker may be a linker described in U.S. Pat. No. 9,089,614 or PCT Publication WO 2015/038426.

In some embodiments, the linker is a dendritic type linker. In certain embodiments, the dendritic type linker comprises a branching, multifunctional linker moiety. The dendritic linker can have two or more branches. In certain embodiments, the dendritic type linker is used to increase the molar ratio of the drug moiety to the antibody or fragment. In certain embodiments, the dendritic type linker comprises PAMAM dendrimers.

In some embodiments, the linker is a traceless linker or a linker which after cleavage does not leave behind a linker moiety (e.g., an atom or a linker group). Exemplary traceless linkers include, but are not limited to, germanium linkers, silicium linkers, sulfur linkers, selenium linkers, nitrogen linkers, phosphorus linkers, boron linkers, chromium linkers, and phenylhydrazide linkers. In some embodiments, the linker is a traceless aryl-triazene linker as described in Hejesen et al., Org Biomol Chem 11(15):2493-2497 (2013). In some embodiments, the linker is a traceless linker described in Blaney et al., Chem. Rev. 102:2607-2024 (2002). In some embodiments, a linker is a traceless linker as described in U.S. Pat. No. 6,821,783.

In some embodiments, the linker comprises a functional group that exerts steric hindrance at the site of bonding between the linker and a conjugating moiety (e.g., any of ADC-A, B, C, E, F, and H-T described herein). In some embodiments, the steric hindrance is a steric hindrance around a disulfide bond. An exemplary linker that exhibits steric hindrance may be, e.g., a heterobifunctional linker (e.g., as described herein). In some embodiments, a linker that exhibits steric hindrance comprises SMCC and SPDB.

In some embodiments, the linker is an acid cleavable linker. In some embodiments, the acid cleavable linker comprises a hydrazone linkage, which is susceptible to hydrolytic cleavage. In some embodiments the acid cleavable linker comprises a thiomaleamic acid linker. In some embodiments, the acid cleavable linker is a thiomaleamic acid linker as described in Castaneda et al., Chem. Commun. 49:8187-8189 (2013).

In some embodiments, the linker is a linker described in any of U.S. Pat. Nos. 6,884,869, 7,498,298, 8,288,352, 8,609,105, and 8,697,688; any of U.S. Patent Publications 2014/0127239, 2013/028919, 2014/286970, 2013/0309256, 2015/037360; and 2014/0294851; or any of PCT Publications WO 2015/057699, WO 2014/080251, WO 2014/197854, WO 2014/145090, and WO 2014/177042.

Suitable linkers for use in the immunoconjugates of the invention may include, e.g., linkers that are intracellularly cleavable with high extracellular stability. In certain embodiments, the linker comprises a functional group that allows for attachment of the linker to any of the antibodies or fragments described herein (e.g., a maleimide derivative). In certain embodiments, the linker (or precursor) comprises 6-maleimidocaproyl (MC), maleimidopropanoyl (MP), valine-citrulline (VC), alanine-phenylalanine (AP), p-aminobenzyloxycarbonyl (PAB), N-succinimidyl 4-(2-pyridylthio) pentanoate (SPP), N-succinimidyl 4-(N-maleimidomethyl) cyclohexane-1-carboxylate (SMCC), N-succinimidyl (4-iodo-acetyl) aminobenzoate (SIAB), 6-maleimidocaproyl-valine-citrulline (MC-VC), 6-maleimidocaproyl-valine-citrulline-p-aminobenzyloxycarbonyl (MC-VC-PAB), N-succinimidyl-1-carboxylate-valine-citrulline-p-aminobenzyloxycarbonyl (SC-VC-PAB), 6-maleimidocaproyl-polyethylene glycol-valine-citrulline (MC-PEG4-VC), 6-maleimidocaproyl-polyethylene glycol-valine-alanine (MC-PEG4-VA), or MC-PEG8-VC-PAB. In some embodiments, the linker prior to conjugation reaction (aka linker precursor) is 6-maleimidocaproyl-valine-citrulline-p-aminobenzyloxycarbonyl (MC-VC-PAB), or N-succinimidyl-1-carboxylate-valine-citrulline-p-aminobenzyloxycarbonyl (SC-VC-PAB). In some embodiments, the linker is a 6-maleimidocaproyl (MC) linker. In some embodiments, the linker is a maleimidopropanoyl (MP) linker. In some embodiments, the linker is a valine-citrulline (VC) linker. In some embodiments, the linker is an alanine-phenylalanine (AP) linker. In some embodiments, the linker is a p-aminobenzyloxycarbonyl (PAB) linker. In some embodiments, the linker is an N-succinimidyl 4-(2-pyridylthio) pentanoate (SPP) linker. In some embodiments, the linker is an N-succinimidyl 4-(N-maleimidomethyl) cyclohexane-1-carboxylate (SMCC) linker. In some embodiments, the linker is an N-succinimidyl (4-iodo-acetyl) aminobenzoate (SIAB) linker. In some embodiments, the linker is a 6-maleimidocaproyl-valine-citrulline (MC-VC) linker. In some embodiments, the linker is a 6-maleimidocaproyl-valine-citrulline-p-aminobenzyloxycarbonyl (MC-VC-PAB) linker. In some embodiments, the linker is an N-succinimidyl-1-carboxylate-valine-citrulline-p-aminobenzyloxycarbonyl (SC-VC-PAB) linker. In some embodiments, the linker further comprises a spacer between the linker and the cytotoxic moiety. In some embodiments, the spacer is a heteroatom. In some embodiments, the spacer is an alkyl chain. In some embodiments, the spacer is an alkyl chain comprising one or more heteroatoms. In some embodiments, the spacer is a carbonyl group. In some embodiments, the linker is a homobifuctional linker or a heterobifunctional linker.

In some embodiments, the linkers described herein may be attached to the antibodies or antigen-binding fragments described herein at a naturally occurring amino acid residue such as a lysine or a reduced cysteine. In some embodiments, the linkers can be attached to a non-natural amino acid (e.g., azidophenylalanine, p-acetylphenylalanine, orp-azidomethylphenylalanine) by way of an alkyne/azide “click” reaction, carbonyl condensations, Michael-type additions, and Mizoroki-Heck substitutions. Additional linker sites can be added genetically and may comprise a polypeptide motif that allows enzymatic addition of the linker. Such polypeptide motifs can comprise, e.g., a glutamine tag (e.g., LLQGA (SEQ ID NO: 58)), an aldehyde tag (e.g., CxPxR, where x is any amino acid), a sortase motif (e.g., LPxTG (SEQ ID NO: 59, where x is any amino acid), NPQTN (SEQ ID NO: 60)), or a BirA tag (e.g., GFEIDKVWYDLDA (SEQ ID NO: 61)). Attaching linkers to a glutamine tag may be achieved by using a bacterial transglutaminase. Attaching linkers to an aldehyde tag is achieved by using formylglycine-generating enzyme (FGE), which oxidizes the cysteine residue of the consensus sequence, creating an aldehyde; this aldehyde can be reacted with an aminooxy group on a linker to form a stable oxime. Attaching linkers to a sortase motif can be achieved by using a bacterial transpeptidase that recognizes a C-terminal LPxTG sequence (SEQ ID NO: 59) or NPQTN sequence (SEQ ID NO: 60), cleaves the TG or TN bond, and facilitates—via a thioacyl enzyme-threonine intermediate—the nucleophilic attack of the incoming protein alpha amine on the threonine. The attacking residue can be a glycine dimer or trimer of a linker or added to a linker. Attaching linkers to a sortase motif or a BirA tag can be achieved by using a biotin ligase. In certain embodiments, the linker is not attached at a lysine residue. In certain embodiments, the linker is only attached to a cysteine residue. In certain embodiments, the cysteine residue has been engineered into the antibody by converting one or more non-cysteine residues on the light chain and/or heavy chain to a cysteine. In certain embodiments, cysteine conversion does not interfere with antigen binding. In certain embodiments, selenocysteine is incorporated into the antibody by genetic modification. See, e.g., Sochaj et al., Biotechnology Advances 33:775-784 (2015).

In some embodiments, the linker is conjugated to the anti-ROR1 antibody or fragment by a chemical ligation process. In some embodiments, the linker is conjugated to the anti-ROR1 antibody or fragment by a native ligation. In some embodiments, the conjugation is as described in Dawson et al., Science 266:776-779 (1994); Dawson et al., J. Am. Chem. Soc. 119:4325-4329 (1997); Hackeng et al., PNAS 96:10068-10073 (1999); or Wu et al., Angew. Chem. Int. Ed. 45:4116-4125 (2006). In some embodiments, the conjugation is as described in U.S. Pat. No. 8,936,910. In some embodiments, the linker is conjugated to the anti-ROR1 antibody or fragment either site-specifically or non-specifically via native ligation chemistry.

In some embodiments, the linker is conjugated to the anti-ROR1 antibody or antigen-binding fragment by a site-directed method utilizing a “traceless” coupling technology (Philochem). In some embodiments, the “traceless” coupling technology utilizes an N-terminal 1,2-aminothiol group on the binding moiety which is then conjugated with the antibody or fragment thereof containing an aldehyde group. See, e.g., Casi et al., JACS 134(13):5887-5892 (2012). In some embodiments, the linker is conjugated to the anti-ROR1 antibody or fragment by a site-directed method utilizing an unnatural amino acid incorporated into the binding moiety. In some embodiments, the unnatural amino acid comprises p-acetylphenylalanine (pAcPhe). In some embodiments, the keto group of pAcPhe is selectively coupled to an alkoxy-amine derivatived conjugating moiety to form an oxime bond. See, e.g., Axup et al., PNAS 109(40):16101-16106 (2012).

In some embodiments, the linker is conjugated to the anti-ROR1 antibody or antigen-binding fragment by a sis conjugated to the anti-ROR1 antibody by a site-directed method utilizing an enzyme-catalyzed process. In some embodiments, the site-directed method utilizes SMARTag™ technology (Redwood). In some embodiments, the SMARTag™ technology comprises generation of a formylglycine (FGly) residue from cysteine by formylglycine-generating enzyme (FGE) through an oxidation process under the presence of an aldehyde tag and the subsequent conjugation of FGly to an alkylhydraine-functionalized amino acid molecule via hydrazino-Pictet-Spengler (HIPS) ligation. See, e.g., Wu et al., PNAS 106(9):3000-3005 (2009) and Agarwal et al., PNAS 110(1):46-51 (2013)).

In some embodiments, the enzyme-catalyzed process comprises microbial transglutaminase (mTG). In certain embodiments, the linker is conjugated to the anti-ROR1 antibody or fragment by utilizing a microbial transglutaminase catalyzed process. In some embodiments, mTG catalyzes the formation of a covalent bond between the amide side chain of a glutamine within the recognition sequence and a primary amine of a functionalized amino acid molecule. In some embodiments, mTG is produced from Streptomyces mobarensis. See, e.g., Strop et al., Chemistry and Biology 20(2):161-167 (2013). In some embodiments, the linker is conjugated to the anti-ROR1 antibody by a carbohydrate-based chemical reaction. In the strategy of carbohydrate-based conjugation, the first step is usually to introduce new bioorthogonal functionalities to facilitate conjugation of the antibody to a drug. Strategies that can be used to introduce bioorthogonal functionalities onto the carbohydrate moiety for bioconjugation (glyco-conjugation) include but are not limited to chemical oxidation of glycans; enzymatic and chemo-enzymatic modification of glycans; and metabolic engineering of the carbohydrate moiety. Chemical approaches may use sodium periodate (NaIO4) to oxidize cis-glycol groups of e.g., galactose or sialic acid to generate aldehydes, which then can be coupled with hydrazide- or primary amine functionalized molecules to create acid-labeled hydrazones or with aminooxy groups to form oximes. Enzymatic and chemo-enzymatic approaches treat the sugar residue with neuraminidase (Neu) and galactose oxidase (Gal Oxi) to formate aldehyde functionalities. Continuous treatment of the antibody with β1,4-galactosyltransferase (Gal T)/α2,6-sialyltransferase (Sial T) can yield homogeneously sialylated antibodies. The resulting antibodies then can be selectively oxidized to the corresponding aldehyde functionalities, and if sialic acid derivatives are used, these antibodies can be used as selective bioorthogonal handles. Similarity, the antibody may be treated with β-galactosidase (Gal) and a mutant Gal T to mediate the attachment of bioorthogonal azide- or keto-galactoses to generate homogeneous G2 glycan patterns which possess non-natural functionalities. Another method makes non-canonical thio-fucose derivatives that can be incorporated into the glycan of antibodies by feeding the cells with the bioorthogonal sugar generating expressed antibodies that display thiol functionalities.

Linkers can be conjugated to the anti-ROR1 antibodies and antigen-binding fragments of the current disclosure in multiple ways. Generally a linker and a cytotoxic moiety are synthesized and conjugated before attachment to an antibody. One method of attaching a linker-drug conjugate to an antibody involves reduction of solvent-exposed disulfides with dithiothreitol (DTT) or tris (2-carboxyethyl)phosphine (TCEP), followed by modification of the resulting thiols with maleimide-containing linker-drug moieties (e.g., 6-maleimidocaproyl-valine-citrulline-p-aminobenzyloxycarbonyl (MC-VC-PAB)). A native antibody contains 4 inter-chain disulfide bonds and 12 intra-chain disulfide bonds, as well as unpaired cysteines. Thus, antibodies modified in this way can comprise greater than one linker-drug moiety per antibody. In certain embodiments, the immunoconjugates described herein comprise at least 1, 2, 3, 4, 5, 6, 7, or 8, 9, or 10 linker/drug moieties. In certain embodiments, the immunoconjugates described herein comprise 1 to 10, 1 to 9, 1 to 8, 1 to 7, 1 to 6, 1 to 5, 1 to 4, 1 to 3, 1 to 2, linker/drug moieties, or 1 linker/drug moiety. In cases where the linker is branched and can each attach to multiple drug moieties, the ratio of the drug moiety to the antibody will be higher than using an unbranched linker.

In some embodiments, the linker is of the formula:

wherein

X is C₂₋₈ alkyl;

Y is —(CH₂CH₂O)_(q)CH₂CH₂-;

W is an amino acid unit;

Z is

n is 0 or 1;

p is 0 or 1;

q is an integer from 0 to 12;

u is an integer from 0 to 5; and

v is 0 or 1; wherein ** indicates the point of attachment to the drug moiety (D); and

* indicates the point of attachment to the antibody or fragment (Ab).

In some embodiments, p is 0. In some embodiments, p is 1. In some embodiments, p is 1 and X is —(CH₂)₂—. In some embodiments, p is 1 and X is —(CH₂)₃-. In some embodiments, p is 1 and X is —(CH₂)₄-. In some embodiments, p is 1 and X is —(CH₂)₅-. In some embodiments, p is 1 and X is —(CH₂)₆-. In some embodiments, q is an integer from 1 to 12. In some embodiments, q is an integer from 4 to 12. In some embodiments, q is an integer from 4 to 8. In some embodiments, q is 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12. In some embodiments, W is an amino acid unit comprising naturally occurring amino acid residues, as well as minor amino acids and non-naturally occurring amino acid analogs. In some embodiments, W is selected from alanine, valine, leucine, isoleucine, methionine, phenylalanine, tryptophan, proline, lysine protected or not with acetyl or formyl, arginine, arginine protected or not with tosyl or nitro groups, histidine, ornithine, ornithine protected with acetyl or formyl, and citrulline. In some embodiments, u is 1, 2, 3, 4, or 5. In some embodiments, u is 0. In some embodiments, v is 0. In some embodiments, v is 1.

In some embodiments, the linker is of the formula:

wherein

X is C₂₋₈ alkyl;

Y is —(CH₂CH₂O)_(q)CH₂CH₂-;

W is an amino acid unit;

Z is

n is 0 or 1;

p is 0 or 1;

q is an integer from 0 to 12;

u is an integer from 0 to 5; and

v is 0 or 1; wherein ** indicates the point of attachment to the drug moiety (D); and

* indicates the point of attachment to the antibody or fragment (Ab).

In some embodiments, n is 0. In some embodiments, n is 1. In some embodiments, X is —(CH₂)₂—. In some embodiments, X is —(CH₂)₃—. In some embodiments, X is —(CH₂)₄—. In some embodiments, X is —(CH₂)₅—. In some embodiments, X is —(CH₂)₆—. In some embodiments, W is an amino acid unit comprising naturally occurring amino acid residues, as well as minor amino acids and non-naturally occurring amino acid analogs. In some embodiments, W is selected from alanine, valine, leucine, isoleucine, methionine, phenylalanine, tryptophan, proline, lysine protected or not with acetyl or formyl, arginine, arginine protected or not with tosyl or nitro groups, histidine, ornithine, ornithine protected with acetyl or formyl, and citrulline. In some embodiments, w is 1, 2, 3, 4, or 5. In some embodiments, w is 0. In some embodiments, v is 0. In some embodiments, v is 1.

In some embodiments, the immunoconjugate optionally further comprises an endosomolytic moiety. In some cases, the endosomolytic moiety is a cellular compartmental release component, such as a compound capable of releasing from any of the cellular compartments known in the art, such as the endosome, lysosome, endoplasmic reticulum (ER), Golgi apparatus, microtubule, peroxisome, or other vesicular bodies with the cell. In some cases, the endosomolytic moiety comprises an endosomolytic polypeptide, an endosomolytic polymer, an endosomolytic lipid, or an endosomolytic small molecule. In some cases, the endosomolytic moiety comprises an endosomolytic polypeptide. In other cases, the endosomolytic moiety comprises an endosomolytic polymer. In some embodiments, an endosomolytic polymer described herein is a pH-responsive endosomolytic polymer. A pH-responsive polymer comprises a polymer that increases in size (swells) or collapses depending on the pH of the environment. Polyacrylic acid and chitosan are examples of pH-responsive polymers. In some embodiments, an endosomolytic moiety described herein is a membrane-disruptive polymer. In some cases, the membrane-disruptive polymer comprises a cationic polymer, a neutral or hydrophobic polymer, or an anionic polymer. In some embodiments, the membrane-disruptive polymer is a hydrophilic polymer. In some embodiments, p(alkylacrylic acids) include poly(propylacrylic acid) (polyPAA), poly(methacrylic acid) (PMAA), poly(ethylacrylic acid) (PEAA), and poly(propyl acrylic acid) (PPAA). In some embodiments, a p(alkylacrylic acid) described herein may be a p(alkylacrylic acid) described in Jones, et al., Biochemistry Journal 372:65-75 (2003). In some embodiments, a pH-responsive membrane-disruptive polymer comprises p(butyl acrylate-co-methacrylic acid). See, e.g., Bulmus, et al., Journal of Controlled Release 93:105-120 (2003); and Yessine et al., Biochimica et Biophysica Acta 1613:28-38 (2003). In some embodiments, a pH-responsive membrane-disruptive polymer comprises p(styrene-alt-maleic anhydride). See, e.g., Henry et al., Biomacromolecules 7:2407-2414 (2006). In some embodiments, a pH-responsive membrane-disruptive polymer comprises a pyridyldisulfide acrylate (PDSA) polymer such as poly(MAA-co-PDSA), poly(EAA-co-PDSA), poly(PAA-co-PDSA), poly(MAA-co-BA-co-PDSA), poly(EAA-co-BA-co-PDSA), or poly(PAA-co-BA-co-PDSA) polymer. See, e.g., El-Sayed et al., Journal of Controlled Release 104:417-427 (2005); or Flanary et al., Bioconjugate Chem. 20:241-248 (2009). In some embodiments, the endosomolytic moiety is a lipid (e.g., a fusogenic lipid). In some embodiments, a molecule of Formula (I): A-X-B-Y-C is further conjugated with an endosomolytic lipid (e.g., fusogenic lipid). Exemplary fusogenic lipids include 1,2-dileoyl-sn-3-phosphoethanolamine (DOPE), phosphatidylethanolamine (POPE), palmitoyloleoylphosphatidylcholine (POPC), (6Z,9Z,28Z,31Z)-heptatriaconta-6,9,28,31-tetraen-19-ol (Di-Lin), N-methyl(2,2-di((9Z,12Z)-octadeca-9,12-dienyl)-1,3-dioxolan-4-yl)methanamine (DLin-k-DMA) and N-methyl-2-(2,2-di((9Z,12Z)-octadeca-9,12-dienyl)-1,3-dioxolan-4-yl)ethanamine (XTC). Exemplary small molecules suitable as endosomolytic moieties include, but are not limited to, quinine, chloroquine, hydroxychloroquines, amodiaquins (carnoquines), amopyroquines, primaquines, mefloquines, nivaquines, halofantrines, quinone imines, or any combination thereof.

The term “linkage,” as used herein, refer to a bond or chemical moiety formed from a chemical reaction between the functional group of one molecular entity and another molecule entity. Such bonds may include, but are not limited to, covalent and non-covalent bonds, while such chemical moieties may include, but are not limited to, esters, carbonates, carbamates, imines phosphate esters, hydrazones, acetals, orthoesters, peptide linkages, and oligonucleotide linkages. Hydrolytically stable linkage means that the linkage is substantially stable in water and does not react with water at useful pH values, including but not limited to under physiological conditions, for an extended period of time, perhaps even indefinitely. Hydrolytically unstable or degradable linkage means that the linkage is degradable in water or in aqueous solutions, including, for example, blood. Enzymatically unstable or degradable linkage means that the linkage can be degraded by one or more enzymes. By way of example only, PEG and related polymers may include degradable linkages in the polymer backbone or in the linker group between the polymer backbone and one or more of the terminal functional groups of the polymer molecule. Such degradable linkages include, but are not limited to, ester linkages formed by the reaction of PEG carboxylic acids or activated PEG carboxylic acids with alcohol groups on a biologically active agent, wherein such ester groups generally hydrolyze under physiological conditions to release the biologically active agent. Other hydrolytically degradable linkages include but are not limited to carbonate linkages; imine linkages resulted from reaction of an amine and an aldehyde; phosphate ester linkages formed by reacting an alcohol with a phosphate group; hydrazone linkages which are reaction product of a hydrazide and an aldehyde; acetal linkages that are the reaction product of an aldehyde and an alcohol; orthoester linkages that are the reaction product of a formate and an alcohol; peptide linkages formed by an amine group, including but not limited to, at an end of a polymer such as PEG, and a carboxyl group of a peptide; and oligonucleotide linkages formed by a phosphoramidite group, including but not limited to, at the end of a polymer, and a 5′ hydroxyl group of an oligonucleotide.

1.7 Exemplary ROR1 Immunoconjugates

In some embodiments, the ADC of the present invention has the structure:

wherein Ab is an antibody and D is a drug moiety. In some embodiments, the ADC has the structure:

wherein Ab is an antibody and D is monomethyl auristatin E (MMAE). In some embodiments, the ADC has the structure:

wherein Ab is an antibody. In some embodiments, the ADC has the structure:

wherein Ab is an antibody and D is mertansine (also called DM1). In some embodiments, the ADC has the structure:

wherein Ab is an antibody.

In some embodiments, the ADC has the structure:

wherein Ab is an antibody and D is a drug moiety. In some embodiments, the ADC has the structure:

wherein Ab is an antibody and D is mertansine. In some embodiments, the ADC has the structure:

wherein Ab is an antibody.

In some embodiments, the ADC has the structure:

wherein Ab is an antibody and D is a drug moiety. In some embodiments, the ADC has the structure:

wherein Ab is an antibody and D is a dimeric pyrrolobenzodiazepine (PBD). In some embodiments, the ADC has the structure:

wherein Ab is an antibody.

In some embodiments, the ADC has the structure:

wherein Ab is an antibody and D is a drug moiety. In some embodiments, the ADC has the structure:

wherein Ab is an antibody and D is mertansine. In some embodiments, the ADC has the structure:

wherein Ab is an antibody.

In some embodiments, the ADC has the structure:

wherein Ab is an antibody and D is a drug moiety. In some embodiments, the ADC has the structure:

wherein Ab is an antibody and D is MMAE. In some embodiments, the ADC has the structure:

wherein Ab is an antibody. In some embodiments, the ADC has the structure:

wherein Ab is an antibody and D is mertansine. In some embodiments, the ADC has the structure:

wherein Ab is an antibody.

In some embodiments, the ADC has the structure:

wherein Ab is an antibody and D is a drug moiety. In some embodiments, the ADC has the structure:

wherein Ab is an antibody and D is a duocarmycin. In some embodiments, the ADC has the structure:

wherein Ab is an antibody.

In some embodiments, the ADC has the structure:

wherein Ab is an antibody and D is a drug moiety. In some embodiments, the ADC has the structure:

wherein Ab is an antibody and D is an amanitin, such as α-amanitin. In some embodiments, the ADC has the structure:

wherein Ab is an antibody.

In some embodiments, the ADC has the structure:

wherein Ab is an antibody and D is a drug moiety. In some embodiments, the ADC has the structure:

wherein Ab is an antibody and D is PNU159682. In some embodiments, the ADC has the structure:

wherein Ab is an antibody.

In some embodiments, the ADC has the structure:

wherein Ab is an antibody and D is a drug moiety. In some embodiments, the ADC has the structure:

wherein Ab is an antibody and D is PBD. In some embodiments, the ADC has the structure:

wherein Ab is an antibody.

Exemplary immunoconjugates of the present invention are shown in the table below:

TABLE 2 Exemplary Immunoconjugates Construct Conju- ID mAb gation Linker Components Payload ADC-A Ab1 MAL VC PAB MMAE (mc) ADC-E Ab1 SC VC PAB MMAE ADC-B Ab1 SMCC DM1 ADC-C Ab1 MAL VC PAB DM1 ADC-F Ab1 SC VC PAB DM1 ADC-H Ab1 MAL VC PAB azonafide ADC-I Ab1 MAL C6 α-amanitin (mc) ADC-J Ab1 MAL PEG4 VC PAB DMEA Duocarmycin TM ADC-K Ab1 MAL PEG4 VA PBD ADC-L Ab1 MAL DPR VC PAB MMAE ADC-M Ab1 MAL Phe-C4 VC PAB MMAE ADC-N Ab1 MAL PEG4 VC PAB DMEA PNU-159682 ADC-O Ab1 MAL C2-Gly3 EDA PNU-159682 ADC-P Ab1 MAL Phe-C4 VC PAB DMEA PNU-159682 ADC-Q^(#) Ab1* DBCO (PEG2 VC PAB MMAE)2 ADC-R Ab1* DBCO PEG4 VC PAB DMEA PNU-159682 ADC-S D10 MAL VC PAB MMAE ADC-T 4A5 MAL VC PAB MMAE ^(#)Branched linker-payload. *Site-specific conjugation using microbial transglutaminase.

In the above table, the abbreviations are used as follows: maleimide chemistry (MAL); maleimidocaproyl (mc); succinimide chemistry (SC); succinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate) (SMCC); dibenzylcyclooctyne (DBCO); diaminopropionic acid (DPR); benzyl (Phe); polyethylene glycol (PEG); valine-citrulline (VC); valine-alanine (VA); para-amino-benzyloxycarbonyl (self immolative moiety) (PAB); dimethylethylamine (DMEA); ethylene diamine (EDA); monomethyl auristatin E (MMAE); N2′-Deacetyl-N2′-(3-mercaptol-oxopropyl)maytansine (DM1); and pyrrolobenzodiazepine (PBD), [1,2]Diazepino[3,4-e]indole. For ADC-Q and ADC-R, site-specific conjugation of the payload may be made by using microbial transglutaminase.

The chemical structures of immunoconjugates in Table 2 are shown in the table below:

TABLE 3 Chemical Structures of Exemplary Immunoconjugates ADC Structure A

E

B

C

F

H

I

J

K

L

M

N

O

P

Q

R

S

T

2. SYNTHESIS OF IMMUNOCONJUGATES

In some embodiments, the immunoconjugates described herein are prepared as shown in Schemes 1 and 2. MMAE is prepared in 12 steps starting from L-isoleucine as outlined in Scheme 1. As shown in Scheme 2, MMAE prepared in Scheme 1 is coupled with the 4-nitrophenyl carbonate of a FMOC protected valine-citrulline-p-aminobenzyloxycarbonyl (VC-PAB) linker. Deprotection of the FMOC group gives the VC-PAB-MMAE linker-MMAE construct.

Exemplary synthesis methods for the ADCs shown in Table 3 are illustrated in Example 1 below. In ADC-A, C, H through P, S and T, the antibody is covalently bonded to the linker/payload moiety via cysteine residues. In ADC-B, E, and F, the antibody is covalently bonded to the linker/payload moiety (or to the payload for B) via lysine residues. In ADC-Q and R, the antibody is covalently bonded to the linker/payload via glutamine residues.

3. IMMUNOCONJUGATE THERAPY

The immunoconjugates described herein are useful for treating a variety of cancers. ROR1 has been shown to express across many types of tumors, including lymphomas and solid tumors. High proportions of human cancers express ROR1. For example, Zhang et al. showed that 54% ovarian cancers, 57% colon cancers, 77% lung cancers, 90% lymphomas, 89% skin cancers, 83% pancreatic cancers, 73% testicular cancers, 43% bladder cancers, 96% uterus cancers, 90% prostate cancers, and 83% adrenal cancers that they examined had moderate-to-strong staining with the anti-ROR1 antibody 4A5 (Zhang et al., Am J Pathol. 181(6):1903-10 (2012)). Daneshmanesh et al. similarly found near universal expression of ROR1 in CLL and hairy cell leukemia (HCL) and varying degrees of expression in other lymphoid cancers such as mantle cell lymphoma (MCL), diffuse large B-cell lymphoma (DLBCL)/marginal zone lymphoma (MZL), follicular lymphoma (FL), chronic myeloid leukemia (CML), acute myeloid lymphoma (AML), and myeloma (Daneshmanesh et al., Leuk Lymphoma 54(4):843-50 (2013)). Our own studies similarly have shown that substantial proportions of patients with hepatocellular cancers (HCC) or non-small-cell lung cancer (NSCLC) are ROR1-positive. This broad tumor expression pattern of ROR1 renders the immunoconjugates of the present invention useful in treating many hematological cancers and solid tumors, such as those aforementioned. Further, it has been shown that ROR1 expression increases in aggressive cancers and correlates with poor prognosis; thus, immunoconjugates of the present invention are particularly well suited to treat aggressive or advanced cancers. In some embodiments, the immunoconjugates of the invention lead to partial or complete tumor regression. In particular embodiments, the partial or complete tumor regression may be sustained beyond the final dose of immunoconjugate treatment.

The ROR1 immunoconjugates of the invention, such as those made with an antibody that binds to a ROR1 epitope set forth in SEQ ID NO:1 or 2 (e.g., Ab1, Ab2, Ab3, or Ab4), are effective in treating cancers such as solid tumors that are heterogeneous in ROR1 expression. Tumors having as little as 20% of their cells expressing ROR1 can be treated effectively by the ROR1 immunoconjugates; for example, the tumors may have 20% or more, 30% or more, 40% or more, 50% or more, 60% or more, or 70% or more of their cells expressing ROR1. Without wishing to be bound by theory, it is contemplated that the ROR1 immunoconjugates of the invention may cause cell death in ROR1-negative tumor cells through bystander toxicity effect (i.e., the payload released from a dead tumor cell causes cytotoxicity to a neighboring tumor cell), or by enhancing the anti-tumor immunity of the immune system, or both.

“Treat”, “treating” and “treatment” refer to a method of alleviating or abrogating a biological disorder and/or at least one of its attendant symptoms. As used herein, to “alleviate” a disease, disorder or condition means reducing the severity and/or occurrence frequency of the symptoms of the disease, disorder, or condition. Further, references herein to “treatment” include references to curative, palliative and prophylactic treatment. Treatment of cancer encompasses inhibiting cancer growth (including causing partial or complete cancer regression), inhibiting cancer progression or metastasis, preventing cancer recurrence or residual disease, and/or prolonging the patient's survival.

In some embodiments, the cancer treatable by the immunoconjugates described herein is a ROR1-expressing cancer. The ROR1-expressing cancer can be determined by any suitable method of determining gene or protein expression, for example, by histology, flow cytometry, RT-PCR, or RNA-Seq. The cancer cells used for the determination may be obtained through tumor biopsy or through collection of circulating tumor cells. In certain embodiments, if an antibody-based assay such as flow cytometry or immunohistochemistry is used, ROR1-expressing cancers are any cancers with cells that show anti-ROR1 antibody reactivity greater than that of an isotype control antibody. In certain embodiments, if an RNA-based assay is used, ROR1-expressing cancers are those that show an elevated level of ROR1 RNA compared to a negative control cell or cancer that does not express ROR1.

In certain embodiments, the antibodies and immunoconjugates are for use in treating hematological malignancies. In certain embodiments, the antibodies and immunoconjugates are for use in treating solid tumors. The cancer to be treated may be selected from, e.g., lymphoma, small lymphocytic lymphoma, marginal zone lymphoma, marginal cell B-cell lymphoma, Burkitt's lymphoma, mantle cell lymphoma, diffuse large B-cell lymphoma, a non-Hodgkin lymphoma that has undergone Richter's transformation, chronic lymphocytic leukemia, T cell leukemia, osteosarcoma, renal cell carcinoma, hepatocellular carcinoma, colon cancer, colorectal cancer, breast cancer, epithelial squamous cell cancer, melanoma, myeloma, multiple myeloma, stomach cancer, brain cancer, lung cancer, non-small cell lung cancer, pancreatic cancer, cervical cancer, ovarian cancer, liver cancer, bladder cancer, prostate cancer, testicular cancer, thyroid cancer, and head and neck cancer. In certain embodiments, the cancer to be treated can be a cancer that is refractory to other therapeutics (for example, triple negative breast cancer).

In certain embodiments, the methods for treating cancer described herein comprise treatment with an immunoconjugate of the invention and treatment with an additional therapeutic agent or biologically active molecule. Examples of biologically active molecules include, but are not limited to, peptides, proteins, enzymes, small molecule drugs, prodrugs, carbohydrates, imaging agents, lipids, nucleosides, radionuclides, oligonucleotides, toxins, cells, antibiotics, fungicides, anti-viral agents, anti-inflammatory agents, anti-tumor agents, cardiovascular agents, anti-anxiety agents, hormones, growth factors, steroidal agents, microbially derived toxins, and the like. Further examples of biologically active molecules are those listed above under the heading “Cytotoxic drug moieties.”

In certain embodiments, the immunoconjugate and the additional therapeutic agent or biologically active molecule are administered at the same time, e.g., in the same formulation. In certain embodiments, they are administered separately, on the same or different dosing schedules. In some embodiments, the additional therapeutic agent is a vascular endothelial growth factor (VEGF) inhibitor, a Bruton's tyrosine kinase (BTK) inhibitor, an inhibitor of the mammalian target of rapamycin (mTOR), a phosphoinositide 3-kinase (PI3K) inhibitor, a Janus kinase/signal transducers and activators of transcription (Jak/STAT) signaling inhibitor, a B-cell lymphoma 2 (Bcl-2) inhibitor, a spleen tyrosine kinase (SYK) inhibitor, a microtubule inhibitor, an epithelial growth factor receptor (EGFR) inhibitor, a poly ADP ribose polymerase (PARP) inhibitor, an anaplastic lymphoma kinase (ALK) inhibitor, a DNA-repair inhibitor, a DNA cross-linker, a nucleoside analog, or an immunomodulatory agent.

In some embodiments, the additional therapeutic agent is

a) an antibody such as rituximab (anti-CD20) or bevacizumab (anti-VEGF);

b) a Bruton's tyrosine kinase inhibitor such as acalabrutinib or ibrutinib;

c) an mTOR inhibitor such as sapanisertib, everolimus or BEZ235;

d) a PI3K inhibitor such as idelalisib or buparlisib;

e) a Jak/STAT signaling inhibitor such as ruxolitinib;

f) a Bcl-2 inhibitor such as ABT-199/venetoclax, Bcl-2i-1, or Bcl-2i-2;

g) a SYK inhibitor such as fostamatinib;

h) a microtubule inhibitor such as paclitaxel or vincristine;

i) an EGFR inhibitor such as erlotinib;

j) a PARP inhibitor such as olaparib;

k) an ALK inhibitor such as crizotinib;

l) a DNA-repair inhibitor such as carboplatin;

m) a DNA cross-linker such as oxaliplatin/cisplatin;

n) a nucleoside analog such as gemcitabine; or

o) an immunomodulatory drug (IMiD) such as lenalidomide or pomalidomide.

In a specific embodiment, the additional therapeutic agent is venetoclax.

In certain embodiments, an immunoconjugate of the invention and an additional therapeutic agent or biologically active molecule are used in combination to treat CLL, MCL, or a non-Hodgkin lymphoma that has undergone Richter's transformation. In particular embodiments, the additional therapeutic agent or biologically active molecule is, e.g., ibrutinib, acalabrutinib, venetoclax, Bcl-2i-1, Bcl-2i-2, everolimus, sapanisertib, or idelalisib.

Additional examples of the additional therapeutic agent are pacritinib, buparlisib, BEZ235, ruxolitinib, fostamatinib, rituximab, lenalidomide, pomalidomide, paclitaxel, vincristine, erlotinib, crizotinib, carboplatin, oxaliplatin/cisplatin, bevacizumab, and gemcitibine.

In certain embodiments, an immunoconjugate of the invention is used in combination with an immune checkpoint modulator that enhances the patient's immune system. For example, the conjugate is used with an immune checkpoint inhibitor such as an antibody or antibody derivative, an antisense oligonucleotide, a small interfering RNA, an aptamer, or a peptide, targeting programmed death-ligand 1 (PD-L1, also known as B7-H1, CD274), programmed death 1 (PD-1), CTLA-4, PD-L2 (B7-DC, CD273), LAG3, TIM3, 2B4, A2aR, B7H1, B7H3, B7H4, BTLA, CD2, CD27, CD28, CD30, CD40, CD70, CD80, CD86, CD137, CD160, CD226, CD276, DR3, GAL9, GITR, HAVCR2, HVEM, IDO1, IDO2, ICOS (inducible T cell costimulator), KIR, LAIR1, LIGHT, MARCO (macrophage receptor with collagenous structure), PS (phosphatidylserine), OX-40, SLAM, TIGHT, VISTA, VTCN1, or any combination thereof.

It is understood that the immunoconjugates of the invention may be used in a method of treatment as described herein, may be for use in a treatment as described herein, and/or may be for use in the manufacture of a medicament for a treatment as described herein. The invention also provides kits and articles of manufacture comprising the immunoconjugates of the invention as described herein.

4. PHARMACEUTICAL COMPOSITIONS

In some embodiments, the immunoconjugate of the invention may be comprised in a pharmaceutical composition further comprising one or more pharmaceutically acceptable excipients, carriers, and diluents. For example, the antibodies and immunoconjugates of the invention may be administered suspended in a sterile solution (e.g., a solution comprising 0.9% NaCl). In certain embodiments, the solution further comprises one or more of the following: buffers (e.g., acetate, citrate, histidine, succinate, phosphate, bicarbonate and hydroxymethylaminomethane (Tris) buffers); surfactants (e.g., polysorbate 80 (Tween 80), polysorbate 20 (Tween 20), and poloxamer 188); polyols/disaccharide/polysaccharides (e.g., glucose, dextrose, mannose, mannitol, sorbitol, sucrose, trehalose, and dextran 40); amino acids (e.g., glycine and arginine); antioxidants (e.g., ascorbic acid and methionine); and/or chelating agents (e.g., EDTA and EGTA). Any combination of these excipients is also contemplated. In certain embodiments, the immunoconjugates of the invention are shipped/stored lyophilized and reconstituted before administration. In certain embodiments, lyophilized antibody or immunoconjugate formulations comprise a bulking agent such as mannitol, sorbitol, sucrose, trehalose, and/or dextran 40. The lyophilized formulation can be contained in a vial, such as a glass vial. The immunoconjugates, when formulated, whether reconstituted or not, can be buffered at a certain pH, e.g., less than 7.0 (such as a pH between 4.5 and 6.5, between 4.5 and 6.0, between 4.5 and 5.5, between 4.5 and 5.0, or between 5.0 and 6.0).

The immunoconjugates of the invention, as used herein, encompass pharmaceutically acceptable salts or esters of the conjugates. The pharmaceutically acceptable salts may be formed when an acidic proton present in the polypeptides either is replaced by a metal ion, by way of example an alkali metal ion, an alkaline earth ion, or an aluminum ion; or coordinates with an organic base. In addition, the salt forms of the immunoconjugates can be prepared using salts of the starting materials or intermediates. The immunoconjugates described herein may be prepared as pharmaceutically acceptable acid addition salts (which are a type of a pharmaceutically acceptable salt) by reacting the free base form of the polypeptides described herein with a pharmaceutically acceptable inorganic or organic acid. Alternatively, the immunoconjugates described herein may be prepared as pharmaceutically acceptable base addition salts (which are a type of a pharmaceutically acceptable salt) by reacting the free acid form of amino acids in polypeptides described herein with a pharmaceutically acceptable inorganic or organic base.

Pharmaceutically acceptable salts include, but are not limited to: (1) acid addition salts, formed with inorganic acids such as hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid, and the like; or formed with organic acids such as acetic acid, propionic acid, hexanoic acid, cyclopentanepropionic acid, glycolic acid, pyruvic acid, lactic acid, malonic acid, succinic acid, malic acid, maleic acid, fumaric acid, tartaric acid, citric acid, benzoic acid, 3-(4-hydroxybenzoyl)benzoic acid, cinnamic acid, mandelic acid, methanesulfonic acid, ethanesulfonic acid, 1,2-ethanedisulfonic acid, 2-hydroxyethanesulfonic acid, benzenesulfonic acid, 2-naphthalenesulfonic acid, 4-methylbicyclo-[2.2.2]oct-2-ene-1-carboxylic acid, glucoheptonic acid, 4,4′-methylenebis-(3-hydroxy-2-ene-1-carboxylic acid), 3-phenylpropionic acid, trimethylacetic acid, tertiary butylacetic acid, lauryl sulfuric acid, gluconic acid, glutamic acid, hydroxynaphthoic acid, salicylic acid, stearic acid, muconic acid, and the like; and (2) salts formed when an acidic proton present in the parent compound either is replaced by a metal ion, e.g., an alkali metal ion, an alkaline earth ion, or an aluminum ion; or coordinates with an organic base. Acceptable organic bases include ethanolamine, diethanolamine, triethanolamine, tromethamine, N-methylglucamine, and the like. Acceptable inorganic bases include aluminum hydroxide, calcium hydroxide, potassium hydroxide, sodium carbonate, sodium hydroxide, and the like. The corresponding counterions of the pharmaceutically acceptable salts may be analyzed and identified using various methods including, but not limited to, ion exchange chromatography, ion chromatography, capillary electrophoresis, inductively coupled plasma, atomic absorption spectroscopy, mass spectrometry, or any combination thereof.

In some embodiments, a pharmaceutical composition of the invention includes multiparticulate formulations. In some embodiments, the pharmaceutical composition includes nanoparticle formulations. In some embodiments, nanoparticles comprise cMAP, cyclodextrin, and/or lipids. In some cases, nanoparticles comprise solid lipid nanoparticles, polymeric nanoparticles, self-emulsifying nanoparticles, liposomes, microemulsions, and/or micellar solutions. Additional exemplary nanoparticles include, but are not limited to, paramagnetic nanoparticles, superparamagnetic nanoparticles, metal nanoparticles, fullerene-like materials, inorganic nanotubes, dendrimers (such as with covalently attached metal chelates), nanofibers, nanohorns, nano-onions, nanorods, nanoropes and/or quantum dots. In some embodiments, a nanoparticle is a metal nanoparticle, e.g., a nanoparticle of scandium, titanium, vanadium, chromium, manganese, iron, cobalt, nickel, copper, zinc, yttrium, zirconium, niobium, molybdenum, ruthenium, rhodium, palladium, silver, cadmium, hafnium, tantalum, tungsten, rhenium, osmium, iridium, platinum, gold, gadolinium, aluminum, gallium, indium, tin, thallium, lead, bismuth, magnesium, calcium, strontium, barium, lithium, sodium, potassium, boron, silicon, phosphorus, germanium, arsenic, antimony, and combinations, alloys or oxides thereof.

Any method for administering immunoconjugates accepted in the art may be employed. The pharmaceutical compositions of the invention are typically suitable for parenteral administration. As used herein, “parenteral administration” of a pharmaceutical composition includes any route of administration characterized by physical breaching of a tissue of a subject and administration of the pharmaceutical composition through the breach in the tissue, thus generally resulting in the direct administration into the blood stream, into muscle, or into an internal organ. Parenteral administration thus includes, but is not limited to, administration of a pharmaceutical composition by injection of the composition, by application of the composition through a surgical incision, by application of the composition through a tissue-penetrating non-surgical wound, and the like. In particular, parenteral administration is contemplated to include, but is not limited to, subcutaneous, intraperitoneal, intramuscular, intrasternal, intravenous, intraarterial, intrathecal, intraventricular, intraurethral, intracranial, intratumoral, and intrasynovial injection or infusions; and kidney dialytic infusion techniques. Regional perfusion is also contemplated. Particular embodiments include the intravenous and subcutaneous routes.

5. ARTICLES OF MANUFACTURE AND KITS

The present invention also provides articles of manufacture, e.g, kits, comprising a container (e.g., a single-use or multi-use container) containing a pharmaceutical composition of the present immunoconjugate), optionally an additional biologically active molecule (e.g., another therapeutic agent), and instructions for use. The immunoconjugate and additional biologically active molecule can be packaged separately in suitable packing such as a vial or ampule made from non-reactive glass or plastic. In certain embodiments, the vial or ampule holds lyophilized powder comprising the immunoconjugate or additional therapeutic agent or biologically active molecule. In certain embodiments, the vial or ampule holds a concentrated stock (e.g., 2×, 5×, 10× or more) of the immunoconjugate or biologically active molecule. In certain embodiments, the articles of manufacture such as kits include a medical device for administering the immunoconjugate and/or biologically active molecule (e.g., a syringe and a needle); and/or an appropriate diluent (e.g., sterile water and normal saline). The present invention also includes methods for manufacturing said articles.

6. CERTAIN EMBODIMENTS

Certain embodiments of the invention are further illustrative below.

-   1. An immunoconjugate of the formula:

Ab-((L)_(m)-(D))_(n)

or a pharmaceutically acceptable salt thereof,

wherein:

-   -   i. Ab is an antibody or antigen binding fragment thereof which         binds the ROR1 protein;     -   ii. L is a linker;     -   iii. D is a drug moiety selected from an anti-tubulin agent, a         DNA alkylating agent, a DNA cross-linking agent, a DNA         intercalating agent, and an RNA polymerase II inhibitor;     -   iv. m is 0 or 1; and     -   v. n is an integer from 1 to 10.

-   2. An immunoconjugate of the formula:

Ab-((L)_(m)-(D))_(n)

or a pharmaceutically acceptable salt thereof,

wherein:

-   -   i. Ab is an antibody or antigen binding fragment thereof which         binds the ROR1 protein;     -   ii. L is a cleavable linker;     -   iii. D is a drug moiety selected from an anti-tubulin agent, a         DNA alkylating agent, a DNA cross-linking agent, a DNA         intercalating agent, and an RNA polymerase II inhibitor;     -   iv. m is 0 or 1; and     -   v. n is an integer from 1 to 10

-   3. An immunoconjugate of the formula:

Ab-((L)_(m)-(D))_(n)

or a pharmaceutically acceptable salt thereof,

wherein:

-   -   i. Ab is an antibody or antigen binding fragment thereof which         binds the ROR1 protein;     -   ii. L is a linker, wherein the linker is not attached to a         lysine amino acid residue;     -   iii. D is a drug moiety selected from an anti-tubulin agent, a         DNA alkylating agent, a DNA cross-linking agent, a DNA         intercalating agent, and an RNA polymerase II inhibitor;     -   iv. m is 0 or 1; and     -   v. n is an integer from 1 to 10.

-   4. The immunoconjugate of any one of embodiments 1-3, wherein D is     an anti-tubulin agent.

-   5. The immunoconjugate of embodiment 4, wherein the anti-tubulin     agent comprises an auristatin, a dolastatin, or analog thereof.

-   6. The immunoconjugate of embodiment 5, wherein the auristatin or     dolastatin comprises auristatin E, monomethyl auristatin E (MMAE) or     monomethyl auristatin F (MMAF),     dimethylvaline-valine-dolaisoleuine-dolaproine-phenylalanine-p-phenylenediamine     (AFP), 5-benzoylvaleric acid-auristatin E ester (AEVB), or (AEB).

-   7. The immunoconjugate of embodiment 6, wherein the auristatin     comprises monomethyl auristatin E (MMAE).

-   8. The immunoconjugate of embodiment 4, wherein the anti-tubulin     agent comprises a maytansine, maytansinoid, or analog thereof.

-   9. The immunoconjugate of embodiment 8, wherein the maytansinoid     comprises ansamitocin, mertansine/emtansine (DM1), or     ravtansine/soravtansine (DM4).

-   10. The immunoconjugate of embodiment 9, wherein the maytansinoid     comprises mertansine/emtansine (DM1).

-   11. The immunoconjugate of embodiment 9, wherein the maytansinoid     comprises ravtansine/soravtansine (DM4).

-   12. The immunoconjugate of embodiment 1, wherein D is a DNA     alkylating agent.

-   13. The immunoconjugate of embodiment 12, wherein the DNA alkylating     agent comprises duocarmycin.

-   14. The immunoconjugate of embodiment 1, wherein D is a DNA     cross-linking agent.

-   15. The immunoconjugate of embodiment 14, wherein the DNA     cross-linking agent comprises pyrrolobenzodiazepine (PBD).

-   16. The immunoconjugate of embodiment 1, wherein D is a DNA     intercalating agent.

-   17. The immunoconjugate of embodiment 16, wherein the DNA     intercalating agent comprises PNU-159682.

-   18. The immunoconjugate of embodiment 1, wherein D is an RNA     polymerase II inhibitor.

-   19. The immunoconjugate of embodiment 18, wherein the RNA polymerase     II inhibitor comprises α-amanitin.

-   20. The immunoconjugate of embodiment 1, wherein D is two different     drug moieties covalently linked.

-   21. The immunoconjugate of embodiment 20, wherein D is any two or     more of a DNA alkylating agent, a DNA cross-linking agent, a DNA     intercalating agent, an RNA polymerase II inhibitor, or an     anti-tubulin agent covalently linked to one another.

-   22. The immunoconjugate of any one of embodiments 1 to 21, wherein     the antibody or antigen binding fragment thereof comprises a     chimeric, humanized, deimmunized, or human antibody, or antigen     binding fragment thereof.

-   23. The immunoconjugate of any one of embodiments 1 to 22, wherein     the antibody or antigen binding fragment comprises a Fab, F(ab)₂, or     scFv.

-   24. The immunoconjugate of any one of embodiments 1 to 23, wherein     the immunoconjugate further comprises a second antibody or antigen     binding fragment thereof, wherein the second antibody or antigen     binding fragment thereof specifically binds to an epitope distinct     from the antibody or antigen binding fragment.

-   25. The immunoconjugate of any one of embodiments 1 to 24, wherein     the antibody or antigen binding fragment thereof comprises:     -   a. a heavy chain CDR1 (HCDR1) amino acid sequence set forth in         SEQ ID NO: 7, a heavy chain CDR2 (HCDR2) amino acid sequence set         forth in SEQ ID NO: 8, a heavy chain CDR3 (HCDR3) amino acid         sequence set forth in SEQ ID NO: 9, a light chain CDR1 (LCDR1)         amino acid sequence set forth in SEQ ID NO: 10, a light chain         CDR2 (LCDR2) amino acid sequence set forth in SEQ ID NO: 11, and         a light chain CDR3 (LCDR3) amino acid sequence set forth in SEQ         ID NO: 12;     -   b. a HCDR1 amino acid sequence set forth in SEQ ID NO: 27, a         HCDR2 amino acid sequence set forth in SEQ ID NO: 28, a HCDR3         amino acid sequence set forth in SEQ ID NO: 29, a LCDR1 amino         acid sequence set forth in SEQ ID NO: 30, a LCDR2 amino acid         sequence set forth in SEQ ID NO: 31, and a LCDR3 amino acid         sequence set forth in SEQ ID NO: 32; or     -   c. a HCDR1 amino acid sequence set forth in SEQ ID NO: 37, a         HCDR2 amino acid sequence set forth in SEQ ID NO: 38, a HCDR3         amino acid sequence set forth in SEQ ID NO: 39, a LCDR1 amino         acid sequence set forth in SEQ ID NO: 40, a LCDR2 amino acid         sequence set forth in SEQ ID NO: 41, and a LCDR3 amino acid         sequence set forth in SEQ ID NO: 42.

-   26. The immunoconjugate of any one of embodiments 1 to 24, wherein     the antibody or antigen binding fragment thereof comprises: a heavy     chain variable region comprising an amino acid sequence at least 80%     identical to that set forth in SEQ ID NO: 6, and a light chain     variable region comprising an amino acid sequence at least 80%     identical to that set forth in SEQ ID NO: 5.

-   27. The immunoconjugate of any one of embodiments 1 to 26, wherein     the antibody binds ROR1 with a K_(D) of less than about 40 nM.

-   28. The immunoconjugate of any one of embodiments 1 to 26, wherein     the antibody binds ROR1 with a K_(D) of less than about 1 nM.

-   29. The immunoconjugate of any one of embodiments 1 to 26, wherein     the antibody binds ROR1 with an affinity greater than about 10 times     that of antibody D10, wherein antibody D10 comprises an     immunoglobulin light chain amino acid sequence set forth in SEQ ID     NO: 25, and an immunoglobulin heavy chain amino acid sequence set     forth in SEQ ID NO: 26.

-   30. The immunoconjugate of any one of embodiments 1 to 26, wherein     the antibody binds ROR1 with an affinity greater than about 50 times     that of antibody D10, wherein antibody D10 comprises an     immunoglobulin light chain amino acid sequence set forth in SEQ ID     NO: 25, and an immunoglobulin heavy chain amino acid sequence set     forth in SEQ ID NO: 26.

-   31. The immunoconjugate of any one of embodiments 1 to 30, wherein L     is selected from a cleavable linker, a non-cleavable linker, a     hydrophilic linker, a procharged linker, and a dicarboxylic acid     based linker.

-   32. The immunoconjugate of any one of embodiments 1 to 31, wherein L     is selected from 6-maleimidocaproyl (MC), maleimidopropanoyl (MP),     valine-citrulline (VC), alanine-phenylalanine (AP),     p-aminobenzyloxycarbonyl (PAB), N-succinimidyl 4-(2-pyridylthio)     pentanoate (SPP), N-succinimidyl 4-(N-maleimidomethyl)     cyclohexane-1-carboxylate (SMCC), N-succinimidyl (4-iodo-acetyl)     aminobenzoate (SIAB), 6-maleimidocaproyl-valine-citrulline (MC-VC),     6-maleimidocaproyl-valine-citrulline-p-aminobenzyloxycarbonyl     (MC-VC-PAB), and     N-succinimidyl-1-carboxylate-valine-citrulline-p-aminobenzyloxycarbonyl     (SC-VC-PAB).

-   33. The immunoconjugate of any one of embodiments 1 to 31, wherein L     is 6-maleimidocaproyl-valine-citrulline-p-aminobenzyloxycarbonyl     (MC-VC-PAB).

-   34. The immunoconjugate of any one of embodiments 1 to 31, wherein L     is     N-succinimidyl-1-carboxylate-valine-citrulline-p-aminobenzyloxycarbonyl     (SC-VC-PAB).

-   35. The immunoconjugate of any one of embodiments 1 to 31, wherein L     is 6-maleimidocaproyl-valine-citrulline (MC-VC).

-   36. The immunoconjugate of any one of embodiments 1 to 31, wherein L     is N-succinimidyl 4-(N-maleimidomethyl) cyclohexane-1-carboxylate     (SMCC).

-   37. The immunoconjugate of any one of embodiments 1 to 31, wherein L     is a linker of the formula:

wherein

X is C₂₋₈alkyl;

Y is —(CH₂CH₂O)_(q)CH₂CH₂—;

W is an amino acid unit;

Z is

n is 0 or 1;

p is 0 or 1;

q is an integer from 0 to 12;

u is an integer from 0 to 5; and

v is 0 or 1; wherein ** indicates the point of attachment to D; and

* indicates the point of attachment to Ab.

-   38. The immunoconjugate of embodiment 37, wherein X is —(CH₂)₂—. -   39. The immunoconjugate of embodiment 37, wherein X is —(CH₂)₃—. -   40. The immunoconjugate of embodiment 37, wherein X is —(CH₂)₄—. -   41. The immunoconjugate of embodiment 37, wherein X is —(CH₂)₅—. -   42. The immunoconjugate of any one of embodiments 37 to 41, wherein     p is 0. -   43. The immunoconjugate of any one of embodiments 37 to 41, wherein     p is 1. -   44. The immunoconjugate of any one of embodiments 37 to 43, wherein     q is an integer from 4 to 8. -   45. The immunoconjugate of any one of embodiments 37 to 43, wherein     q is 4. -   46. The immunoconjugate of any one of embodiments 37 to 43, wherein     q is 8. -   47. The immunoconjugate of any one of embodiments 37 to 46, wherein     n is 0. -   48. The immunoconjugate of any one of embodiments 37 to 46, wherein     n is 1. -   49. The immunoconjugate of any one of embodiments 37 to 48, wherein     v is 0. -   50. The immunoconjugate of any one of embodiments 37 to 48, wherein     v is 1. -   51. The immunoconjugate of any one of embodiments 37 to 50, wherein     u is an integer from 2 to 4. -   52. The immunoconjugate of any one of embodiments 37 to 51, wherein     W is selected from alanine, valine, leucine, isoleucine, methionine,     phenylalanine, tryptophan, proline, lysine protected or not with     acetyl or formyl, arginine, arginine protected or not with tosyl or     nitro groups, histidine, ornithine, ornithine protected with acetyl     or formyl, and citrulline. -   53. The immunoconjugate of any one of embodiments 37 to 52, wherein     u is 0. -   54. The immunoconjugate of any one of embodiments 1 to 53, further     comprising a spacer between L and D. -   55. The immunoconjugate of any one of embodiments 1 to 54, wherein     the immunoconjugate further comprises at least one additional     linker-drug moiety of the formula -(((L)_(m)-(D))_(n))_(x)

wherein:

-   -   L is a linker;     -   D is a drug moiety selected from an anti-tubulin agent, a DNA         alkylating agent, a DNA cross-linking agent, a DNA intercalating         agent, or an RNA polymerase II inhibitor;     -   m is 0 or 1;     -   n is an integer from 1 to 10; and     -   x is an integer from 1 to 10.

-   56. A pharmaceutical composition comprising the immunoconjugate of     any one of embodiments 1 to 55 and a pharmaceutically acceptable     excipient, diluent, or carrier.

-   57. The pharmaceutical composition of embodiment 56, wherein the     pharmaceutical composition is formulated for intravenous     administration.

-   58. The pharmaceutical composition of embodiment 56, wherein the     pharmaceutical composition is formulated for subcutaneous     administration.

-   59. A method of treating cancer comprising administering to an     individual in need thereof a therapeutic dose of the immunoconjugate     of any one of embodiments 1 to 55 or the pharmaceutical composition     of any one of embodiments 56 to 58.

-   60. Use of the immunoconjugate of any one of embodiments 1 to 55 or     the pharmaceutical composition of any one of embodiments 56 to 58     for treating cancer.

-   61. The method of embodiment 59 or the use of embodiment 60, wherein     the cancer expresses ROR1.

-   62. The method or use of embodiment 61, wherein the cancer is     selected from lymphoma, chronic lymphocytic leukemia, small     lymphocytic lymphoma, marginal cell B-cell lymphoma, Burkett's     lymphoma, renal cell carcinoma, colon cancer, colorectal cancer,     breast cancer, epithelial squamous cell cancer, melanoma, myeloma,     stomach cancer, brain cancer, lung cancer, pancreatic cancer,     cervical cancer, ovarian cancer, liver cancer, bladder cancer,     prostate cancer, testicular cancer, thyroid cancer, and head and     neck cancer

-   63. A method of treating an individual with cancer, the method     comprising administering to the individual:     -   a. the immunoconjugate of any one of embodiments 1 to 55; and     -   b. an additional therapeutic agent.

-   64. The method of embodiment 63, wherein the additional therapeutic     agent comprises acalabrutinib, idelalisib, sapanisertib, pacritinib,     ABT-199, buparlisib, everolimus, BEZ235, ruxolitinib, fostamatinib,     rituximab, lenalidomide, paclitaxel, vincristine, ibrutinib,     erlotinib, crizotinib, carboplatin, oxaliplatin/cisplatin,     bevacizumab, or gemcitibine.

-   65. The method of embodiment 63 or 64, wherein the cancer expresses     ROR1.

-   66. The method of embodiment 65, wherein the cancer comprises     lymphoma, chronic lymphocytic leukemia, small lymphocytic lymphoma,     marginal cell B-cell lymphoma, Burkett's lymphoma, renal cell     carcinoma, colon cancer, colorectal cancer, breast cancer,     epithelial squamous cell cancer, melanoma, myeloma, stomach cancer,     brain cancer, lung cancer, pancreatic cancer, cervical cancer,     ovarian cancer, liver cancer, bladder cancer, prostate cancer,     testicular cancer, thyroid cancer, and head and neck cancer.

-   67. The method of any one of embodiments 63 to 66, wherein the     immunoconjugate and the additional therapeutic agent are     administered separately to the individual with cancer.

-   68. A pharmaceutical composition comprising the immunoconjugate as     described in any one of embodiments 1 to 55 and the additional     therapeutic agent as described in embodiment 64.

-   69. The pharmaceutical composition of embodiment 68, for use in a     method of any one of embodiments 63 to 67.

-   70. A kit comprising the immunoconjugate as described in any one of     embodiments 1 to 55 and the additional therapeutic agent as     described in embodiment 64, wherein said immunoconjugate and     additional therapeutic agent are in suitable packaging.

-   71. The kit of embodiment 70, for use in a method of any one of     embodiments 63 to 67.

Unless the context requires otherwise, throughout the specification and claims, the word “comprise” and variations thereof, such as, “comprises” and “comprising,” are to be construed in an open, inclusive sense, that is, as “including, but not limited to.” As used in this specification and the appended claims, the singular forms “a,” “an,” and “the” include plural referents unless the content clearly dictates otherwise. It should also be noted that the term “or” is generally employed in its sense including “and/or” unless the content clearly dictates otherwise. As used herein the term “about” refers to a numerical range that is 10%, 5%, or 1% plus or minus from a stated numerical value within the context of the particular usage. Further, headings provided herein are for convenience only and do not interpret the scope or meaning of the claimed embodiments.

All publications and patents mentioned herein are incorporated herein by reference in their entirety for the purpose of describing and disclosing, for example, the constructs and methodologies that are described in the publications, which might be used in connection with the presently described inventions. The publications discussed herein are provided solely for their disclosure prior to the filing date of the present application. Nothing herein is to be construed as an admission that the inventors of the subject invention are not entitled to antedate such disclosure by virtue of prior invention or for any other reason.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood to one of ordinary skill in the art to which the inventions described herein belong. Any methods, devices, and materials similar or equivalent to those described herein can be used in the practice or testing of the inventions described herein.

EXAMPLES

The following examples illustrate representative embodiments of the present invention and are not meant to be limiting in any way.

Example 1: Synthesis of Exemplary Immunoconjugates 1. Conjugation of Ab1 Cysteines in ADC-A, C, and H Through P

Conjugation of Ab1 with MC-VC-PAB-MMAE (ADC-A) was performed at multiple scales (2 mg, 30 mg, and 350 mg) with similar results. For the smallest scale, 2 mg Ab1 (10 mg/mL in PBS, pH 6.5) was treated with 2.50 equivalents (eq) of tris(2-carboxyethyl)phosphine (TCEP, 5 mM) in conjugation buffer solution in a water bath at 37° C. for 2 hours. The PBS buffer contained 15.75 mM Na₂HPO₄, 34.25 mM NaH₂PO₄, 2 mM EDTA, and 50 mM NaCl, at pH 6.5. Subsequently, the reaction was cooled to 4° C. Next, 7 eq MC-VC-PAB-MMAE in N,N-dimethylacetamide (DMA) was added and the mixture was left at 4° C. for an additional 1 hour. The buffer was exchanged with 20 mM histidine, pH 5.5 (MMAE buffer) by using a spin desalting column (40 kD, 0.5 mL). The number of MMAE drug molecules linked per antibody molecule (DAR) was determined using HIC-HPLC, SEC-HPLC, RP-HPLC, and UV/Vis and is summarized in Table 4. Consistent results were obtained at all scales, with DAR ranging from 3.89 to 5.09 on average, depending on the methodology used.

TABLE 4 Characterization of ADC-A Scale Aggregate Recovery D0* DAR (mg) (%) (%) (%) UV SEC HIC RP 2 3.47 85.6 6.06 4.33 4.81 3.95 3.89 30 3.50 86.1 4.75 4.44 5.09 4.21 4.09 350 3.51 90.2 5.12 4.40 4.96 4.12 3.99 *D0: unconjugated antibody.

Other linkers and payloads (ADC-C, and ADC-H through ADC-P) were conjugated to Ab1 in a similar fashion, and the resulting DARs are summarized in Table 5.

TABLE 5 Average DAR for Additional ADC Constructs Construct DAR Methodology ADC-C 6.33 UV 6.62 SEC 3.97 HIC 4.15 RP 4.24 MS ADC-H 1.98 UV 1.93 SEC ADC-I 4.1 SEC/UV ADC-J 2.1 HIC ADC-K 1.9 PLRP ADC-L 3.9 HIC ADC-M 4.1 HIC ADC-N 4.1, 1.9 PLRP ADC-O 1.8 SEC ADC-P 1.9 PLRP

2. Conjugation of Ab1 Lysines in ADC-B, E, and F

Ab1 in PBS (pH 6.5) was combined with SC-VC-PAB-MMAE (ADC-E) or SC-VC-PAB-DM1 (ADC-F) in DMA and placed on a rotating platform at 10 rpm at 22° C. for 2-4 hours. For ADC-B, Ab1 in PBS (pH 7.0) was combined with SMCC-DM1 and placed on a rotating platform at 10 rpm at 22° C. for 2-4 hours. Unreacted payload was removed through buffer exchange, using Amicon ultrafiltration (50 kDa, 0.5 mL). ADC-B was exchanged into 20 mM succinic acid (pH 5.0). DAR values were determined using SEC-HPLC, MS, and UV/Vis and are summarized in Table 6. When linker-payload was used at ˜11 eq, the DARs approached 4.0 following 4 hours incubation.

TABLE 6 Characterization of ADC-B, ADC-E and ADC-F Scale Aggregate Recovery D0 DAR Construct (mg) (%) (%) (%) UV SEC MS ADC-B 80 2.93 — 5.86 3.18 3.20 3.0 ADC-E 60 3.94 82.1 1.85 5.08 6.14 4.2 ADC-F 75 2.92 59.0 7.44 5.51 5.72 3.0

3. Site-Specific Conjugation of Ab1 in ADC-Q and R

ADC-Q and ADC-R were synthesized using site-specific conjugation technology (Dennler et al., Bioconjugate Chemistry, 25(3):569-578 (2014) and U.S. Patent Publication 2016/0022833). Ab1 was first deglycosylated by incubation with 6 U/mg protein of N-glycosidase F (PNGase F) overnight at 37° C. The deglycosylated antibody was purified by Protein A chromatography and formulated for the bacterial transglutaminase modification reaction. Next, 10-40 eq of 11-azido-3,6,9-trioxaundecan-1-amine (azido-PEG3-amine) per glutamine was added to 1-5 mg/ml of Ab1 in PBS (pH 7.4) and the reaction was initiated by the addition of 2-6 U/mL of microbial transglutaminase and incubated overnight at 37° C. Subsequently, excess azido-PEG3-amine was removed by Protein A chromatography. Finally, 50-200 μM dibenzylcyclooctyne (DBCO)-containing drug payload (1.25 to 5 mol equiv. per azide group) was added to 20 μM azide-activated Ab1 and incubated at room temperature for 0.5-6 hours, forming Ab1-payload conjugates linked via a triazole moiety. Excess DBCO-containing payload was removed. Characterization of ADCs synthesized in this manner demonstrated DARs of 4.4 for ADC-Q and 2.1 for ADC-R (Table 7).

TABLE 7 Average DAR for Additional ADC Constructs Construct DAR Methodology ADC-Q 4.4 HIC ADC-R 2.1 PLRP

Example 2: Antibody and Immunoconjugate Binding to ROR1-Positive Cells

The binding to tumor cells by antibodies Ab1 and 4A5, as well as ADC constructs derived from those antibodies (ADC-A and ADC-T), was evaluated. First, the binding of Ab1 to two ROR1-positive human tumor cell lines representing different cancer types was tested. The Jeko-1 cell line, from mantle cell lymphoma, is a suspension cell line, while the MDA-MB-231 cell line, from triple negative breast cancer, is an adherent cell line. The cell lines have different levels of ROR1 expression, with Jeko-1 cells displaying about 13507 cell surface copies and the MDA-MB-231 cells displaying about 21015 cell surface copies. The Jeko-1 (ATCC Cat. No. CRL-3006) and MDA-MB-231 (ATCC Cat. No. CCL-227) cell lines were purchased from the American Type Culture Collection and were maintained according to the ATCC recommendations.

Jeko-1 cells (FIG. 2A) or MDA-MB-231 cells (FIG. 2B) were incubated with 0, 1, 10, 100, or 1000 ng/mL of Ab1 for 20 minutes on ice. After washing off unbound antibody, the cells were incubated with secondary anti-human IgG antibody labeled with phycoerythrin (PE) for an additional 20 minutes. The amount of PE fluorescence was measured using the BDFACS Verse analyzer and FlowJo V10 software. The PE signal for the highest concentration was used as the maximal binding signal to calculate the % maximal binding. EC₅₀ was calculated using GraphPad Prism 7. The receptor binding assay resulted in EC₅₀ values of 13.6 ng/ml in Jeko-1 cells and 32.8 ng/ml in MDA-MB-231 cells.

Next, the binding of Ab1 was compared to ADC-A (FIGS. 2A and 2B). Binding was compared on both Jeko-1 and MDA-MB-231 cell lines. The binding of ADC-A to Jeko-1 cells (FIG. 2A, squares) and MDA-MB-231 cells (FIG. 2B, squares) was very similar to the binding of the unconjugated, parental antibody Ab1 (FIG. 2A, 17 ng/mL vs. 13.6 ng/mL and FIG. 2B, 48.5 ng/mL vs. 32.8 ng/mL). The similarity between the EC₅₀ values of unconjugated Ab1 and ADC-A demonstrates that drug conjugation had minimal impact on Ab1's binding to the target cells.

The binding of Ab1 was compared to 4A5, an antibody that binds an epitope on ROR1 distinct from Ab1's epitope (FIG. 3A). Ab1 (EC₅₀=16.39 ng/mL; FIG. 3A, open circles) bound Jeko-1 cells with a higher affinity/avidity than did 4A5 (EC₅₀=120.6 ng/mL; FIG. 3A, open squares). The binding of the ADC constructs was compared to the corresponding unconjugated parental antibodies (Ab1 versus ADC-A and 4A5 versus ADC-T). ADC-A bound with an EC₅₀ of 21.57 ng/mL while ADC-T bound with an EC₅₀ of 192.7 ng/mL. The similarity between the EC₅₀ values of unconjugated antibodies and the corresponding ADC constructs demonstrates again that drug conjugation had minimal impact on the antibodies' binding to the target cells.

In a different experiment, the binding of Ab1 to Jeko-1 cells was also compared to the binding of D10, a lower affinity antibody that binds the same epitope as Ab1 (FIG. 3B). Ab1 (EC₅₀=16.5 ng/mL; FIG. 3B, open circles) bound Jeko-1 cells with a higher affinity/avidity than did D10 (EC₅₀=250 ng/mL; FIG. 3B, open triangles). In addition, the binding of the ADC constructs was compared to that of the corresponding unconjugated parental antibodies (Ab1 versus ADC-A and D10 versus ADC-S). ADC-A bound with an EC₅₀ of 22.1 ng/mL (FIG. 3B, closed circles) while ADC-S bound with an EC₅₀ of 150 ng/mL (FIG. 3B, closed triangles). The similarity between the EC₅₀ values of unconjugated antibodies and the corresponding ADC constructs demonstrates again that drug conjugation had minimal impact on the antibodies' binding to the target cells.

In a similar fashion, the binding of all other ADC constructs to ROR1-positive cells was evaluated. All constructs described herein were found to bind ROR1-positive cells.

Example 3: Internalization of ROR1 Antibody and Immunoconjugates

Multiple methods were used to determine antibody internalization. In one approach, internalization was measured using a pulse-chase methodology that quantitates non-internalized, cell surface antibody at 15, 30, 60, 120, and 240 minutes using flow cytometry. Jeko-1 cells were grown and harvested prior to confluency, washed with cold PBS and resuspended at 1×107 in cold FACS buffer (PBS+2% FBS−Fisher/Gibco Cat. No. 16140071). 1×10⁶ cells were added to microcentrifuge tubes or wells. Internalization of Ab1 and ADC constructs by the cells was determined at saturating antibody concentrations, using 30 μg/ml for Jeko-1 cells and 100 μg/mL for MDA-MB-231 cells. 10× stock solutions of ADC constructs or Ab1 were prepared (300 μg/ml for use with Jeko-1 cells or 1 mg/ml for use with MDA-MB-231 cells) and 10 μL of sample or buffer control was added to the following (adding additional tubes for each time point):

-   -   a) Unstained     -   b) Secondary antibody only     -   c) Negative Control hIgG mAb at 30 μg/ml—time 0     -   d) Positive Control ADC construct or Ab1 at 30 μg/ml—time 0     -   e) ADC construct or Ab1—time point 1     -   f) ADC construct or Ab1—time point 2, etc.

Cells and antibody were incubated on ice for 20 minutes, spun at 300×g for 4 minutes, washed twice with 200 μL of FACS buffer, and resuspended in 100 μL of FACS buffer. The samples were incubated at 37° C. for 15, 30, 60, 120, and 240 minutes, as indicated above. Internalization was terminated by transferring the samples to ice. A time course for antibody internalization was generated for each test article as described above, after which antibody remaining on the cell surface was detected using a PE-labeled secondary antibody. Cells were spun at 250×g, washed twice with FACS buffer and resuspended in 100 μL of FACS buffer. The secondary antibody (goat anti-human IgG-PE, Fc-gamma specific—eBiosciences, Cat. No. 12-4998) was diluted 1:2000 (10× stock) in FACS buffer and 10 μL/tube was added to the appropriate tubes. Cells were incubated on ice for 20 minutes, washed twice with FACS buffer and resuspended in 100 μL of fix buffer (4% paraformaldehyde in PBS). Subsequently, FACS analysis was performed. The median fluorescent intensity (MFI) was quantitated and the degree of receptor internalization was determined using the amount of the antibody or ADC present at each time point when compared to the amount present at time=0. The dashed line represents background staining (secondary antibody only).

Ab1 bound to and was rapidly internalized by both Jeko-1 (FIG. 4A) and MDA-MB-231 (FIG. 4B) cells. Internalization of 80-90% of the bound antibody was observed in less than 60 minutes for MDA-MB-231 or 120 minutes for Jeko-1. Notably, the addition of linker and payload to Ab1 did not negatively impact binding or internalization, as demonstrated with ADC-A and ADC-B (FIG. 4A, compare ADC-A and ADC-B with Ab1, and FIG. 4B, compare ADC-A with Ab1).

In a similar fashion, the internalization characteristics of other ADC constructs with different linkers and payloads were evaluated using Jeko-1 cells. All of the constructs displayed similar kinetics of internalization compared to Ab1, consistent with the above observation that the linkers and payloads used did not impact the antibody characteristics (data not shown).

We also evaluated the internalization properties of an ADC construct (ADC-T) utilizing a ROR1 antibody (4A5) that recognizes an epitope distinct from Ab1, but with linker and payload chemistries identical to those of ADC-A. ADC-T bound Jeko-1 cells but was internalized at a slower rate and to a lesser extent than did ADC-A (data not shown). These data demonstrate the importance of the antibody and its epitope in determining the internalization properties of the ADC construct.

Internalization of Ab1 was also characterized using conventional immunofluorescent staining methods and MDA-MB-231 cells. In this approach, Ab1 was loaded at the cell surface and the cells were subsequently fixed and surface Ab1 was quantitated. In addition, a second sample of the cells was permeabilized and total Ab1 (surface and intracellular) was quantitated. In this protocol, the primary antibody was allowed to incubate without a secondary antibody, eliminating the possibility that the secondary antibody might influence internalization. Surface staining was observed. After permeabilization, clear and distinct punctate intracellular staining of Ab1 was observed. Furthermore, by utilizing a lysosome-specific tracker, it was demonstrated that internalized Ab1 co-localized with the lysosomal pathway. This finding is consistent with a mode of antibody internalization that primarily occurs by the lysosome/endosome pathway. Finally, the rate of internalization was quantitated. When cells were subjected to continuous exposure to Ab1, the initial rate was approximately twice the average rate, and the terminal rate was approximately half the average rate (FIG. 5). This indicates that there is an initial rapid phase of clearing the cell surface receptor followed by a slower process of binding and internalizing receptor that is newly expressed on the cell surface. The newly expressed cell surface receptor may be recycled, expressed from intracellular stores, and/or newly synthesized. The extent and character of co-localization of Ab1 was similar to EGFR, showing substantially lysosomal co-localization after 4 hours.

Example 4: Measurement of Cell Surface ROR1 Expression

Internalization of antibodies was measured using pulse-chase approaches that detected non-internalized, cell surface antibody at various time points (2, 5, 10, 15, 20, 30, 60, 120 and 240 minutes). In this study, in addition to measuring non-internalized cell surface antibody, the expression of ROR1 on the cell surface was quantitated either by (1) re-staining with Ab1 and a secondary antibody or (2) by using a second, labeled anti-ROR1 antibody that recognizes an epitope distinct from that of Ab1.

For the first approach, internalization of Ab1 on Jeko-1 cells was performed as described in Example 3 (one sample was processed at each time point to quantitate antibody remaining on the cell surface). Additionally, a second sample at each time point was rapidly re-stained using saturating levels of Ab1 (30 μg/mL) while maintaining the cells on ice. Subsequently, the cells were washed and surface antibody was quantitated using the same secondary antibody used for internalization measurements. As shown previously, Ab1 was rapidly internalized (FIG. 6, squares). By contrast, while quantitation of cell surface ROR1 initially showed a small decrease in the first 10 minutes, subsequent measurements indicated ROR1 surface expression was restored to initial or slightly higher levels (FIG. 6, circles). These data are consistent with recycling of ROR1 to the cell surface dissociated from antibody, or with rapid upregulation of ROR1 through de novo synthesis or trafficking of intracellular stores. This experiment was repeated using Jeko-1 (FIG. 7A), MDA-MB-468 (FIG. 7B), and MDA-MB-231 (FIG. 7C) cells, with similar results.

An alternative approach to measuring cell surface ROR1 expression in conjunction with Ab1 internalization studies was tested. Specifically, cell surface ROR1 expression was quantitated at 0, 0.5, 1, 2, and 4 hours by staining cells directly with a labeled anti-ROR1 antibody that binds an epitope distinct from that of Ab1. ROR1 surface expression on Jeko-1 cells treated with ADC-A, M, N, and P was assessed. Consistent with the results obtained using the previously described approach, ROR1 expression was maintained, or slightly increased, while cells were being treated with Ab1-based ADC constructs (data not shown).

These data indicate that Ab1 and its immunoconjugates may be internalized effectively by ROR1-positive cells. Further, the persistent expression of ROR1 on the cell surface demonstrates that ROR1 is an excellent target for delivering cytotoxic agents to cancer cells through an ADC of the present invention.

Example 5: Potency of Immunoconjugates In Vitro

The ADC-A, B, C, E, and F immunoconjugates, which had different cytotoxic moieties and linker chemistry, were analyzed for this Example. ROR1 binding by these conjugates was tested in cell culture to define the potency of various linker-cytotoxic agent combinations. Three different types of cancer cell lines were tested: B-lymphoma cell-lines TMD-8, HBL-1, and DOHH2; triple negative breast cancer cell lines HCC1187, MDA-MB-468, Bt549; and ovarian cancer cell lines A2008, TOV112D, JHOM1, and SKOvr3.

Cells were cultured in log phase growth and distributed into 96-well plates. Each cell line was plated at a slightly different cell density, ranging from 5×10³ to 50×10⁴ cells/well. Cells in duplicate were incubated with 3-fold serial dilution of a particular immunoconjugate (10, 3.33, 1.11, 0.37, 0.12, 0.041, 0.014, and 0.0045 μM) for 72 hours at 37° C. and 5% CO₂. After treatment, cells were incubated with an equal volume of CellTiter-Glo® reagent (Promega Inc.) for 15 minutes at room temperature and viability was determined by a luminometer. Curves and EC₅₀ values were generated in GraphPad Prism using a sigmoidal dose response non-linear regression fit. The data from these experiments are summarized in Table 8. FIGS. 8A-8I show representative IC₅₀ plots for all five immunoconjugates tested against the indicated cell lines.

TABLE 8 Cytotoxicity of Exemplary Immunoconjugates in vitro* Cancer Type Cell line ADC-A ADC-B ADC-C ADC-E ADC-F B-cell TMD-8 122 50 >1000 94.3 >1000 lymphoma HBL-1 118 70.1 >1000 147 >1000 DOHH2 129 78.3 >1000 89.6 >1000 Triple HCC1187 6.8 6.2 >1000 >1000 negative MDA- 23.5 18.8 >1000 80.1 >1000 breast MB-468 cancer Bt549 13.5 20.2 >1000 74.4 >1000 Ovarian A2008 125 49 >1000 >1000 cancer TOV112D 62.4 34.2 >1000 57.8 >1000 JHOM1 15.6 24.3 >1000 32.9 >1000 SKOvr3 62.6 78.7 >1000 200.6 >1000 *Data represented as IC₅₀ in nM. Empty cells: not done.

Example 6: Additional Data on Potency of Immunoconjugates In Vitro

This Example presented additional data on the potency of the various ADCs in inducing cell death of a variety of cancer cell lines. Cells were grown and distributed into 96-well plates as described in Example 5. Cells in duplicate were incubated with 3-fold serial dilution of a particular immunoconjugate (660, 220, 73.3, 24.4, 8.14, 2.71, 0.91, and 0.3 nM) for 72 hours at 37° C. and 5% CO₂. In some experiments, cells were incubated with an immunoconjugate for 96 hours instead of 72 hours (values highlighted with “*”). After treatment, cell viability was determined as described in Example 5. The data from these experiments is summarized in Tables 9.1 through 9.17 below. Cell surface ROR1 expression was assessed by quantitative flow cytometry and ROR1 expression was characterized as MFI>30 or MFI<30 (MFI: mean fluorescent intensity). The data demonstrate that payload-sensitive cancer cells expressing ROR1 responded to a number of the tested ADCs in the cell death assays described herein. While many of the ADC-responsive cancer cells expressed high levels of ROR1 (MFI>30), some cells expressing lower levels of ROR1 (MFI<30) were also sensitive to certain ADCs.

The MEC1 cell line, which is derived from B-chronic lymphocytic leukemia in prolymphocytoid transformation, was transfected with either an expression vector encoding human ROR1 or a control vector, and stable cell lines were created using selection media containing G418. ROR1-transfected MEC1 cells had significantly greater proportions of cells in S/G2/M phase than did control-transfected cells 16 hours after being transferred from serum-free medium to complete growth medium, implying that expression of ROR1 increased the relative proportions of cells undergoing cell division. Consistent with this, ROR1⁺ MEC1 cells had significantly greater numbers of cells ≥48 hours after being transferred from serum-free medium than did comparably seeded cultures of MEC1 cells that did not express ROR1. Increased levels of p-AKT and p-CREB were also observed in MEC1 cells made to express ROR1 relative to MEC1 cells transfected with control vector.

TABLE 9.1 Cytotoxicity of ADC-A in vitro ADC-A Cancer Binding ADC-A MMAE ROR1 Cell Line Type K_(D) (nM) EC₅₀ (nM) EC₅₀ (nM) Expression Mec-vector CLL     558   0.5 MFI < 30 Mec-ROR CLL     208   0.6 MFI > 30 Jeko-1 MCL 0.10 22.4*, 40.4*, 186.7 <0.0001 MFI > 30 Mino MCL 19.5, 27.7* <0.0001 MFI > 30 TMD-8 DLBCL     122*  <0.0001 MFI < 30 HBL-1 DLBCL     118*  <0.0001 MFI > 30 DOHH2 DLBCL 129, 48.3* 0.007 MFI > 30 OCI-Ly18 DLBCL     95   0.003 MFI > 30 U2932 DLBCL     176   0.006 MFI < 30 OCI-Ly19 DLBCL     199   WSU-DLCL DLBCL     68   0.06 MFI > 30 KMS-PE MM 43.3, 89 RPMI-8229 MM     174   OPM2 MM     123   L363 MM     244   NCI-H929 MM     179   HCC1187 TNBC 2.3*, 6.8 <0.0001 MFI > 30 HCC1806 TNBC 13.4*, 38.1 <0.0001 MFI > 30 MDA-MB-468 TNBC 23.4, 21.7*, 82.5, 23.5* <0.0001 MFI > 30 MDA-MB-231 TNBC 0.32 58.4*, 106, 46.7* <0.0001 MFI > 30 Bt549 TNBC     13.5 0.047 MFI > 30 Hs578T TNBC     22.9 <0.0001 DU4475 TNBC     248   2.5 MFI > 30 HCC1937 TNBC >1,000   >1,000 MFI > 30 MCF-7 Breast 198.4*, 289.0, >1000* <0.0001 MFI < 30 H1975 NSCLC      9.7 <0.0001 MFI > 30 H460 NSCLC     61.5 <0.0001 MFI > 30 A549 NSCLC     53.4 0.06 MFI > 30 Hep-G2 HCC >1,000   >1,000 A4573 EWS     15.3 <0.0001 MFI > 30 SKES EWS     15.3 0.03 MFI > 30 U2OS OS     223   1.2 MFI > 30 HOS OS 126, 148 0.06 MFI > 30 SAOS OS 15.6, 26.3 0.05 MFI > 30 FaDu HN >1,000   >1,000 A2008 Ovarian     125   0.03 OvCar4 Ovarian     283   24.2 MFI > 30 TOV112D Ovarian     62.4 0.04 MFI > 30 JHOM1 Ovarian     15.6 0.01 MFI > 30 SKOvr3 Ovarian     62.6 0.01 MFI > 30 ES2 Ovarian     170   1.1 MFI > 30 A2780 Ovarian     64   0.08 MFI > 30 Bx-PC3 Pancreatic     47.4 0.04 MFI > 30 As-PC1 Pancreatic >1,000   0.04 MFI > 30 Ramos Burkitt's     649.8 <0.0001 MFI < 30

TABLE 9.2 Cytotoxicity of ADC-E in vitro ADC-E MMAE Cell Line Cancer Type EC₅₀ (nM) EC₅₀ (nM) TMD-8 DLBCL 94.3 <0.0001 HBL-1 DLBCL 147 <0.0001 DOHH2 DLBCL 89.6 0.007 HCC1187 TNBC <0.0001 MDA-MB-468 TNBC 80.1 <0.0001 Bt549 TNBC 74.4 0.047 A2008 Ovarian 0.03 TOV112D Ovarian 57.8 0.04 JHOM1 Ovarian 32.9 0.01 SKOvr3 Ovarian 200.6 0.01

TABLE 9.3 Cytotoxicity of ADC-B in vitro ADC-B DM1 Cell Line Cancer Type EC₅₀ (nM) EC₅₀ (nM) TMD-8 DLBCL 50 1.4 HBL-1 DLBCL 70.1 9.3 DOHH2 DLBCL 78.3 1.78 OCI-Ly18 DLBCL 40 U2932 DLBCL 53.9 OCI-Ly19 DLBCL 40 WSU-DLCL DLBCL >1,000 KMS-12PE MM 72 RPMI-8229 MM 91 OPM2 MM 108 L363 MM 177 NCI-H929 MM 90 HCC1187 TNBC 6.2, 25.1 3.9 HCC1806 TNBC 23.1 MDA-MB-468 TNBC 18.8, 70.1 15.2 MDA-MB-231 TNBC 67.5 Bt549 TNBC 20.2 0.047 Hs578T TNBC 11.7 DU4475 TNBC 105 HCC1937 TNBC >1,000 MCF-7 ER+/PR+/HER2− Breast >1,000 A2008 Ovarian 49 OvCar4 Ovarian 291 >1,000 TOV112D Ovarian 34.2 2.4 JHOM1 Ovarian 24.3 0.01 SKOvr3 Ovarian 78.7 9.1 ES2 Ovarian 117 A2780 Ovarian 36

TABLE 9.4 Cytotoxicity of ADC-C in vitro ADC-C DM1 Cell Line Cancer Type EC₅₀ (nM) EC₅₀ (nM) TMD-8 DLBCL >1,000 1.4 HBL-1 DLBCL >1,000 9.3 DOHH2 DLBCL >1,000 1.78 HCC1187 TNBC >1,000 3.9 MDA-MB-468 TNBC >1,000 15.2 Bt549 TNBC >1,000 0.047 A2008 Ovarian >1,000 TOV112D Ovarian >1,000 2.4 JHOM1 Ovarian >1,000 0.01 SKOvr3 Ovarian >1,000 9.1

TABLE 9.5 Cytotoxicity of ADC-F in vitro ADC-F DM1 Cell Line Cancer Type EC₅₀ (nM) EC₅₀ (nM) TMD-8 DLBCL >1,000 1.4 HBL-1 DLBCL >1,000 9.3 DOHH2 DLBCL >1,000 1.78 HCC1187 TNBC >1,000 3.9 MDA-MB-468 TNBC >1,000 15.2 Bt549 TNBC >1,000 0.047 A2008 Ovarian >1,000 TOV112D Ovarian >1,000 2.4 JHOM1 Ovarian >1,000 0.01 SKOvr3 Ovarian >1,000 9.1

TABLE 9.6 Cytotoxicity of ADC-H in vitro ADC-H Azonafide Cell Line Cancer Type EC₅₀ (nM) EC₅₀ (nM) Jeko MCL 170 MDA-MB-231 TNBC >1,000 A549 NSCLC >1,000 >1,000 Hep-3B HCC >1,000 >1,000

TABLE 9.7 Cytotoxicity of ADC-I in vitro ADC-I Cell Line Cancer Type EC₅₀ (nM) Jeko MCL >1000 DOHH2 DLBCL >1000 KMS-PE MM 266 MDA-MB-468 TNBC >1000 MDA-MB-231 TNBC >1000 MCF-7 ER+/PR+/HER2− Breast >1,000 A549 NSCLC >1,000 Hep-G2 HCC >1,000 U2OS OS >1,000 FaDu HN >1,000

TABLE 9.8 Cytotoxicity of ADC-J in vitro ADC-J Cell Line Cancer Type EC₅₀ (nM) Jeko MCL 59.35 DOHH2 DLBCL 5.975 KMS-PE MM 362 MDA-MB-468 TNBC 146 MDA-MB-231 TNBC >1000 MCF-7 ER+/PR+/HER2− Breast 268 A549 NSCLC >1,000 Hep-G2 HCC >1,000 U2OS OS >1,000 FaDu HN 658.5

TABLE 9.9 Cytotoxicity of ADC-K in vitro ADC-K Cell Line Cancer Type EC₅₀ (nM) Jeko MCL 761.5 DOHH2 DLBCL 247 KMS-PE MM 1,000 MDA-MB-468 TNBC >1000 MDA-MB-231 TNBC >1000 MCF-7 ER+/PR+/HER2− Breast 363 A549 NSCLC >1,000 Hep-G2 HCC >1,000 U2OS OS >1,000 FaDu HN >1,000

TABLE 9.10 Cytotoxicity of ADC-L in vitro ADC-L Binding K_(D) ADC-L MMAE Cell Line Cancer Type (nM) EC₅₀ (nM) EC₅₀ (nM) Mec-vector CLL 535 0.5 Mec-ROR CLL 192 0.6 Jeko MCL 0.15 81.1 <0.0001 Mino MCL 20.5 <0.0001 MDA-MB-468 TNBC 141.5 <0.0001 MCF-7 ER+/PR+/ 232.5* <0.0001 HER2− Breast

TABLE 9.11 Cytotoxicity of ADC-M in vitro ADC-M ADC-M MMAE Binding K_(D) EC₅₀ EC₅₀ Cell Line Cancer Type (nM) (nM) (nM) Mec-vector CLL 569 0.5   Mec-ROR CLL 170 0.6   Jeko MCL 0.12 112.3 <0.0001 Mino MCL 15.8 <0.0001 MDA-MB-468 TNBC 53.1 <0.0001 MCF-7 ER+/PR+/ 164.1 <0.0001 HER2− Breast

TABLE 9.12 Cytotoxicity of ADC-N in vitro ADC-N PNU- Cancer Binding K_(D) ADC-N 159682 Cell Line Type (nM) EC₅₀ (nM) EC₅₀ (nM) Mec-vector CLL 772 Mec-ROR CLL 262 Jeko MCL 0.13 31.7, 20.3 Mino MCL 7.7 DOHH2 DLBCL 3.8 KMS-PE MM 26.75 MDA-MB-468 TNBC 4.1, 29.4 MDA-MB-231 TNBC 71. 0.005 MCF-7 ER+/PR+/ 78.6 HER2− Breast H460 NSCLC 84.4 0.001 A549 NSCLC 30.7 Hep-G2 HCC 20.8 U2OS OS 11.25 FaDu HN 27.05

TABLE 9.13 Cytotoxicity of ADC-O in vitro ADC-O PNU- Binding ADC-O 159682 Cell Line Cancer Type K_(D) (nM) EC₅₀ (nM) EC₅₀ (nM) Mec-vector CLL 296 Mec-ROR CLL 116 Jeko MCL 0.12 71.7 Mino MCL 28 MDA-MB-468 TNBC 85.8 MDA-MB-231 TNBC 782 0.005 MCF-7 ER+/PR+/ 12.65 HER2− Breast H460 NSCLC 365 0.002

TABLE 9.14 Cytotoxicity of ADC-P in vitro ADC-P PNU- Binding ADC-P 159682 Cell Line Cancer Type K_(D) (nM) EC₅₀ (nM) EC₅₀ (nM) Mec-vector CLL 30.3 Mec-ROR CLL 10.4 Jeko MCL 0.11 2.4  Mino MCL 1.2  MDA-MB-468 TNBC 10.4 MDA-MB-231 TNBC 24.7 0.005 MCF-7 ER+/PR+/ 30.9 HER2− Breast H460 NSCLC 39.6 0.001

TABLE 9.15 Cytotoxicity of ADC-Q in vitro ADC-Q Binding ADC-Q MMAE Cell Line Cancer Type K_(D) (nM) EC₅₀ (nM) EC₅₀ (nM) Jeko MCL 0.23   265* <0.0001 Mino MCL   139* <0.0001 HCC1187 TNBC    5.3* <0.0001 HCC1806 TNBC    56.1* <0.0001 MDA-MB-468 TNBC    79.7* <0.0001 MDA-MB-231 TNBC   167.8* <0.0001 HCC1937 TNBC >1,000* >1,000 MCF-7 ER+/PR+/ >1,000* <0.0001 HER2− Breast H1975 NSCLC    26.8* <0.0001 H460 NSCLC   180.1* <0.0001 Bx-PC3 Pancreatic    45.6* 0.04

TABLE 9.16 Cytotoxicity of ADC-R in vitro ADC-R PNU- Binding K_(D) ADC-R 159682 Cell Line Cancer Type (nM) EC₅₀ (nM) EC₅₀ (nM) Mec-vector CLL 183 Mec-ROR CLL  44.6 Jeko MCL 0.25  53.3 Mino MCL  15 MDA-MB-468 TNBC  44.3 MDA-MB-231 TNBC 203 0.005 MCF-7 ER+/PR+/ 167* HER2− Breast H460 NSCLC 191 0.001

TABLE 9.17 Cytotoxicity of ADC-S in vitro ADC-S MMAE Cell Line Cancer Type EC₅₀ (nM) EC₅₀ (nM) Mino MCL 83.3 <0.0001

The data in the tables demonstrate that the tested ADCs are effective against a variety of cancer cell lines with different ROR1 expression levels and different sensitivities to the various payloads.

Example 7: Antigen Dependency of Anti-Proliferative Effects of Exemplary ADCs

The studies described in this Example evaluated the dependency of the tested ADCs on ROR1 for their anti-proliferative effects. Jeko-1 cells were cultured in log phase growth prior to experiment setup. For competition experiments, 5×10⁴ cells were incubated with 100 μg/ml of Ab1 or vehicle control for 2 hours at 37° C. and 5% CO₂. Subsequently, 3, 10, and 30 μg/ml of ADC-A was added and the cells were incubated for an additional 72 hours at 37° C. and 5% CO₂. Each condition was tested in duplicate. Cell viability was determined as described in Example 5.

The data show that ADC-A inhibited cell proliferation in a dose-dependent manner (FIG. 9, black bars). Pre-incubation of the cells with the non-conjugated parental antibody Ab1 inhibited this anti-proliferative activity at all ADC-A concentrations tested (FIG. 9, gray bars), demonstrating that the cell killing was mediated by the binding of ADC-A to its target, ROR1.

Example 8: Anti-Tumor Activities of Exemplary Immunoconjugates In Vivo 1. Efficacy of ADC-A in a TCL1×ROR1 CLL Mouse Model

It has been demonstrated that ROR1 interacts with the T-cell leukemia 1 oncogene (TCL1) and enhances leukemogenesis in Em-TCL1 transgenic mice, and treatment with an anti-ROR1 antibody can impair the engraftment of ROR1×TCL1 leukemia cells (Widhopf et al., PNAS 111: 793-798 (2014)). This study evaluated the activity of ADC-A as compared to vehicle and unconjugated Ab1 in the TCL1×ROR1 CLL mouse model. In the study, ROR1×TCL1 leukemia cells were engrafted into mice via tail vein injection, and the injected mice were randomized into five groups, 8 mice/group. The groups were vehicle control, 10 mg/kg Ab1, and 1, 2, and 5 mg/kg ADC-A. The mice were dosed IV weekly for a total of four doses. The results of this study are shown in Table 10 below.

2. Efficacy of ADC-A and ADC-Q in a MCL Xenograft Mouse Model

Jeko-1 (MCL) cells were engrafted subcutaneously into mice, and the engrafted mice were randomized into five groups, 9 mice/group. The groups were vehicle control, 10 mg/kg Ab1, 20 mg/kg ibrutinib, 5 mg/kg ADC-A, and 5 mg/kg ADC-Q. The mice were dosed IV q4d. The results of this study are shown in Table 10 below.

3. Efficacy of ADC-A in a DLBCL-GCB PDX Mouse Model

Germinal center B-cell like diffuse large B-cell lymphoma (DLBCL-GCB) cells were engrafted subcutaneously into mice, and the engrafted mice were randomized into five groups, 6 mice/group. The groups were vehicle control, 10 mg/kg Ab1, 50 mg/kg venetoclax, 10 mg/kg Ab1 plus 50 mg/kg venetoclax, and 5 mg/kg ADC-A. Ab1 and ADC-A were administered IV, qw and venetoclax was administered PO qd. The results of this study are shown in Table 10 below.

4. Efficacy of ADC-A in a ROR1-Positive Richter's Syndrome PDX Mouse Model

NSG mice were subcutaneously injected in both flank areas with RS9373 cells (patient-derived xenograft or PDX) in a cell suspension in matrigel. When palpable tumors were observed (at approximately 50 mm³), the animals were randomized into three groups, 4 mice/group. The treatment groups were vehicle control, 2.5 mg/kg ADC-A IV q4d, and 5 mg/kg ADC-A IV q4d. The mice received 3 total treatments and tumors were harvested 24 hours after the last treatment. Mean tumor growth inhibition (TGI) was calculated utilizing the following formula:

${TGI} = {\left\lbrack {1 - \frac{\left( {{\overset{\_}{\chi}{Treated}_{({Final})}} - {\overset{\_}{\chi}{Treated}_{({{Day}\mspace{14mu} 1})}}} \right)}{\left( {{\overset{\_}{\chi}{Control}_{({Final})}} - {\overset{\_}{\chi}{Control}_{({{Day}\mspace{14mu} 1})}}} \right)}} \right\rbrack \times 100\%}$

With most tumor types, heterogenous ROR1 expression is observed at the intra-tumoral level. Nonetheless, tumor regression was observed in the ADC-A treated groups. In the RS101 PDX model of Richter lymphoma, 20-30% of the cells were ROR1-positive, but complete and sustained regressions were observed (FIG. 13). The results of this study are shown in Table 10 below.

5. Efficacy of ADC-A in a ROR1-Positive Human TNBC Xenograft Model

NCR mice were subcutaneously injected in the mammary fat pad with MDA-MB-231 cells (ROR1-positive human triple negative breast cancer (TNBC) cells) in a cell suspension in matrigel. When palpable tumors were observed (at approximately 250 mm³), the animals were randomized into three different groups, 9 mice/group: vehicle control, 1 mg/kg ADC-A IV qw, and 5 mg/kg ADC-A IV qw. The mice received 5 total treatments and tumors were harvested 24 h after the last treatment. Mean tumor growth inhibition (TGI) was calculated utilizing the above formula. The results of this study are shown in Table 10 below.

6. Efficacy of ADC-A in Two ROR1-Positive Human TNBC PDX Models

Two PDX models displaying different levels of ROR1 expression were selected for this study. ROR1 expression was based on the level of ROR1 staining via IHC of a tissue microarray representing triplicate cores of TNBC PDX models. The mean level of ROR1 expression as assessed by % ROR1-positive cells were 58% and 38% for human TNBC cells BR5011 and BR5015, respectively.

NOD/SCID nude mice were subcutaneously injected with BR5011 or BR5015 cells in the mammary fat pad. When palpable tumors were observed (mean tumor volume approximately 150 mm³ for BR5011 and approximately 250 mm³ for BR5015), animals were randomized into three groups. The groups for the BR5011 PDX model were vehicle control, 1 mg/kg ADC-A IV q4d, and 5 mg/kg ADC-A IV q4d. The groups for the BR5015 PDX model were vehicle control, 1 mg/kg ADC-A IV qw, and 5 mg/kg ADC-A IV qw. Mean TGI was calculated as described above.

Tumor inhibition and/or regression was observed in the ADC-A treated groups. For example, in the BR5011 TNBC PDX model, which showed ROR1 expression in 58% of the cancer cells, complete and sustained regressions were observed (FIG. 15). The results of this study are shown in Table 10 below.

7. Efficacy of ADC-A, L, M, N, P, R, S and T in a Jeko-1 Human MCL Xenograft Model

Jeko-1 (MCL) cells were engrafted subcutaneously into mice. When tumor size reached 100 mm³, the mice were randomized into nine groups, 9 mice/group. The groups were vehicle control; 1 mg/kg ADC-N, ADC-P or ADC-R; or 5 mg/kg ADC-A, ADC-L, ADC-M, ADC-S, or ADC-T. The mice were dosed IV q4d. The interim results of this study are shown in Table 10 below.

TABLE 10 Results of in vivo Studies with Exemplary Immunoconjugates Study ADC Animal Model Duration Results FIG. 1 ADC-A TCL1 × ROR1 1 month ADC-A inhibited leukemic cell 10 leukemia cells tumor burden in a dose- dependent manner (1, 2, and 5 mg/kg). 2 ADC-A Jeko-1 MCL xenograft 3 weeks ADC-A caused tumor 11 ADC-Q regression while ADC-Q caused tumor growth delay. 3 ADC-A DLBCL-GCB PDX 24 days ADC-A caused tumor 12 regression (104%) with complete regressions in all animals. Ab1 (unconjugated) alone, venetoclax alone, or Ab1 (unconjugated) + venetoclax were ineffective. 4 ADC-A Chemotherapy-resistant 48 days 20-30% of the intra-tumoral 13 PDX model of Richter cells were ROR1-positive, but transformation complete and sustained regressions were observed. ADC-A mean TGI: 78% at 2.5 mg/kg; 90% at 5 mg/kg. 5 ADC-A MDA-MB-231 47 days ADC-A mean TGI: 14 ADC-B xenograft 56.4% at 1 mg/kg; 94.2% at 5 mg/kg. 6 ADC-A Human TNBC PDX 56 days 58% of the intra-tumoral cells 15 model, BR5011 dosing; were ROR1-positive, but 84 days complete and sustained observation regressions were observed at 5 (5 mg/kg mg/kg ADC-A. group) ADC-A mean TGI: 40.1% at 1 mg/kg, complete regression at 5 mg/kg. The 5 mg/kg group was monitored for 28 days post last dose and tumor regression was maintained throughout the observation period. Human TNBC PDX, 17 days ADC-A mean TGI: 16 BR5015 (low ROR1 22% at 1 mg/kg; expression) 75.6% at 5 mg/kg. 7 ADC-A Jeko-1 MCL xenograft Interim results Interim analysis at days at 11 17 ADC-L 14 days and 14 shows that all ADC ADC-M constructs inhibited tumor ADC-N growth and all ADC-treated ADC-P animals demonstrated a ADC-R reduction in average tumor size ADC-S at day 14 compared to day 11. ADC-T This suggests that regression may be observed in all treatment groups by the end of the study (beyond day 14). Animals treated with ADC-A, L, M, and S all displayed significant tumor regression, as shown by the reduction in average tumor size at day 14 compared to pre-treatment at day 0.

In vivo studies 1-7 here show that despite the heterogeneity of ROR1 expression within a tumor and varying levels of ROR1 expression across cancer types, treatment with ADC-A resulted in tumor growth inhibition and even sustained, complete regression in a variety of cancers. For example, in the TNBC PDX model that showed expression of ROR1 in 58% o of the tumor cells, complete and sustained regressions were observed in all tumors (FIG. 15). Similarly, in the RS101 PDX model of Richter lymphoma, 20-30% o of the cells were ROR1-positive, but complete and sustained regressions were observed in all tumors (FIG. 13). These results suggest that the anti-ROR1 immunoconjugates eradicate cancer by not only cytotoxicity to the bound cancer cells, but also bystander toxicity on neighboring cancer cells in the tumor microenvironment, or by upregulating the immune system's anti-cancer effect, or by a combination of these mechanisms. This finding is significant because it indicates that the immunoconjugates of the present invention can be used to treat tumors that have intra-tumoral heterogeneous expression of ROR1.

The present in vivo studies also show that ADC-A was effective in treating drug-resistant cancers. We observed regressions in human tumor xenografts that were resistant to ibrutinib (the Jeko-1 MCL model) or to rituximab-CHOP immunochemotherapy (the Richter transformation model). Since ROR1 is a marker for advanced cancers and that prior chemotherapy appears to increase ROR1 expression, our findings demonstrate the potential of the immunoconjugates of the present invention in treating advanced or aggressive cancers.

8. Further In Vivo Evaluation of ADCs

The in vivo efficacy of the ADCs, e.g., ADC-A, E, F, L, M, N, P, Q, and R may be further evaluated in a ROR1-positive, subcutaneous, human MCL xenograft model using Jeko-1 cells. The animals are dosed IV q4d with vehicle, 1 mg/kg ADC, or 5 mg/kg ADC. Tumor growth and body weights are measured every 2-3 days. Pharmacokinetic analysis of the ADCs of the present invention may be performed to determine standard pharmacokinetic parameters such as antibody C_(max) and half-life.

Example 9: Combination Therapy

The effects of combining ADC-A with other anti-proliferative agents were examined. The initial studies focused on the effects of ADC-A combined with an inhibitor of BTK (ibrutinib, ACP-196/acalabrutinib), Bcl-2 (ABT-199/venetoclax), mTOR (INK128), or PI3K (idelalisib). Additional studies examined the effects of combining ADC-A with two additional Bcl-2 inhibitors, termed Bcl-2i-1 and Bcl-2i-2. Cell lines were tested for both distinct DLBCL subtypes—germinal center B-cell (GCB) and activated B-cell (ABC). The median-effect analysis was used to determine synergism, antagonism, or additivity of inhibition of proliferation of various cell lines treated with ADC-A combined with the anti-proliferative agents. The Combination Index (CI) was determined using the Chou/Talalay equation. The IC₅₀ for each compound was determined in a 72 hour CellTiter-Glo® assay. For combination assays, drugs were used at equimolar ratios (i.e., at the ratio of their IC₅₀ values). CalcuSyn software (by Biosoft) was used for dose effect analysis. A Combination Index of less than 1.1 indicates the treatments are synergistic; 0.9-1.1 indicates the treatments are additive; and greater than 1.1 indicates that the treatments are antagonistic.

ADC-A displayed a synergistic effect with two different BTK inhibitors, ibrutinib (FIG. 18A) and ACP-196/acalabrutinib (FIG. 18B). The synergistic effect was more pronounced with acalabrutinib and with MCL cell lines. Representative data for ADC-A combined with ibrutinib and acalabrutinib on MCL cell line Jeko-1 are shown in FIGS. 19A and 19B, respectively.

ADC-A also displayed a synergistic effect with Bcl-2 inhibitor ABT-199/venetoclax (FIG. 20A). The synergistic effect was pronounced in both MCL and DLBCL cell lines. The activity of ADC-A combined with additional Bcl-2 inhibitors, Bcl2i-1 and Bcl2i-2, was examined on Jeko-1 (FIG. 20B) and Mino (FIG. 20C) cells (both are MCL cell types). ADC-A displayed a synergistic effect with both inhibitors on Jeko-1 cells and an additive effect with both inhibitors on Mino cells. Representative data for ADC-A combined with venetoclax on Jeko-1 are shown in FIG. 21.

ADC-A displayed a synergistic effect with mTOR1/2 inhibitor INK128/sapanisertib (FIG. 22). The synergistic effect was observed on both MCL and DLBCL cell lines. Representative data for ADC-A combined with sapanisertib on Jeko-1 are shown in FIG. 23.

ADC-A displayed a synergistic effect with PI3K inhibitor CAL-101/idelalisib (FIG. 24). The synergistic effect was observed on both MCL and DLBCL cell lines. Representative data for ADC-A combined with idelalisib demonstrating synergistic effects on both subtypes of DLBCL are shown in FIG. 25A (TMD cells, DLBCL-ABC) and FIG. 25B (DOHH2 cells, DLBCL-GCB).

Example 10: Further Evaluation of Combination Therapy

The in vivo efficacy of the ADCs in combination with a Bcl-2 inhibitor may be further assessed in a ROR1-positive human MCL xenograft model using Jeko-1 cells or in a PDX model. Both the ADC and the Bcl-2 inhibitor are dosed at maximum tolerated and sub-optimal doses, alone and in combination as outlined below, in a repeat dose study. Tumor growth and body weights are measured every 2-3 days.

-   -   ADC-A and Bcl-2 inhibitor MTD dosing         -   5 mg/kg ADC-A, IV, q4d         -   100 mg/kg venetoclax (ABT-199), weekly     -   ADC-A and Bcl-2 inhibitor sub-optimal and combination dosing         -   1 mg/kg ADC-A, IV, q4d         -   2 mg/kg ADC-A, IV, q4d         -   50 mg/kg ABT-199, PO, qd         -   100 mg/kg ABT-199, PO, qd         -   1 mg/kg ADC-A, IV, q4d+50 mg/kg ABT-199, PO, qd         -   2 mg/kg ADC-A, IV, q4d+50 mg/kg ABT-199, PO, qd         -   1 mg/kg ADC-A, IV, q4d+100 mg/kg ABT-199, PO, qd         -   2 mg/kg ADC-A, IV, q4d+100 mg/kg ABT-199, PO, qd

Pharmacokinetic analysis is performed to determine standard pharmacokinetic parameters such as antibody C_(max) and half-life.

Example 11: Phase 2 Clinical Study of Anti-ROR1-MMAE Immunoconjugates

The following describes a protocol for a prospective open label Phase 1b/2 clinical trial for anti ROR1-MMAE immunoconjugate therapy.

Inclusion Criteria:

-   -   Eastern Cooperative Oncology Group (ECOG) performance status of         0, 1, or 2     -   Histological diagnosis of CLL/SLL or MCL as documented in         medical records     -   CLL/SLL or MCL has been previously treated and has relapsed         after or progressed during prior therapy     -   Presence of radiographically measurable lymphadenopathy or         extranodal lymphoid malignancy (defined as the presence of ≥1         non-biopsied, non-irradiated lesion that measures ≥2.0 cm in the         longest dimension [LD] and ≥1.0 cm in the longest perpendicular         dimension [LPD] as assessed by computed tomography [CT] or         magnetic resonance imaging [MRI]).     -   Current medical need for therapy due to disease-related         symptoms, lymphadenopathy, organomegaly, extranodal organ         involvement, or progressive disease.     -   Completion of all previous therapy (including surgery,         radiotherapy, chemotherapy, immunotherapy, or investigational         therapy) for the treatment of cancer 1 week before the start of         study therapy.     -   All acute toxic effects of any prior antitumor therapy resolved         to Grade 1 before the start of study therapy (with the exception         of alopecia [Grade 1 or 2 permitted], or neurotoxicity [Grade 1         or 2 permitted], or selected laboratory parameters [Grade 1 or         Grade 2 permitted with exceptions as noted below]).     -   Adequate bone marrow function:         -   a) Absolute neutrophil count (ANC) ≥1.0×109/L (Grade ≤2). b)             Platelet count ≥50×109/L (Grade ≤2). b) Hemoglobin ≥8.0 g/dL             (Grade ≤2) maintained for ≥1 week from any prior             transfusion.     -   Note: Grade ≥3 neutropenia, thrombocytopenia, or anemia is         permitted if the abnormality is related to bone marrow         involvement with hematological malignancy (as documented by bone         marrow biopsy/aspirate obtained since the last prior therapy).     -   Adequate hepatic profile:         -   Serum alanine aminotransferase (ALT)≤3×upper limit of normal             (ULN) (Grade <1).         -   Serum aspartate aminotransferase (AST)≤3×ULN (Grade ≤1).         -   Serum bilirubin ≤1.5×ULN (Grade ≤1).     -   Adequate renal function:         -   a) Estimated creatinine clearance (eClCR)>45 mL/minute (with             eClCR to be calculated by the Cockcroft-Gault formula),             or b) Measured creatinine clearance >45 mL/minute (as             assessed with a 24-hour urine collection).     -   Adequate coagulation profile:         -   Prothrombin time (PT)≤1.5×ULN (Grade≤1).         -   Activated partial thromboplastin time (aPTT)≤1.5×ULN             (Grade≤1).     -   Negative viral serology:         -   Negative human immunodeficiency virus (HIV) antibody.         -   Negative hepatitis B surface antigen (HBsAg) and negative             hepatitis B core (HBc) antibody or undetectable hepatitis B             (HBV) deoxyribonucleic acid (DNA) by quantitative polymerase             chain reaction (PCR) testing.         -   Negative hepatitis C virus (HCV) antibody or negative HCV             ribonucleic acid (RNA) by quantitative PCR.     -   For female subjects of childbearing potential, a negative urine         or serum pregnancy test prior to the start of study therapy.     -   In the judgment of the investigator, participation in the         protocol offers an acceptable benefit-to-risk ratio when         considering current disease status, medical condition, and the         potential benefits and risks of alternative treatments for the         subject's cancer.     -   Willingness and ability of the subject to comply with scheduled         visits, drug administration plan, protocol-specified laboratory         tests, other study procedures (including all bone marrow         biopsy/aspirations and radiographic studies), and study         restrictions.

Exclusion Criteria:

-   -   Known histological transformation to an aggressive lymphoma (ie,         Richter transformation). Note: Biopsy documentation of the         absence or presence of transformation is not required.     -   Known central nervous system malignancy. Note: Central nervous         system imaging is only required in subjects with suspected         central nervous system malignancy.     -   History of another malignancy except for the following:         adequately treated local basal cell or squamous cell carcinoma         of the skin; adequately treated carcinoma in situ without         evidence of disease; adequately treated, papillary, noninvasive         bladder cancer; other cancer that has been in complete remission         for ≥2 years.     -   Significant cardiovascular disease (eg, myocardial infarction,         arterial thromboembolism, cerebrovascular thromboembolism)         within 3 months prior to start of study therapy; angina         requiring therapy; symptomatic peripheral vascular disease; New         York Heart Association Class 3 or 4 congestive heart failure; or         uncontrolled Grade ≥3 hypertension (diastolic blood pressure         ≥100 mmHg or systolic blood pressure ≥160 mmHg) despite         antihypertensive therapy.     -   Significant screening ECG abnormalities, including unstable         cardiac arrhythmia requiring medication, atrial         fibrillation/flutter, left bundle branch block, 2nd-degree         atrioventricular (AV) block type II, 3rd-degree AV block, Grade         ≥2 bradycardia, or corrected QT (QTc) >450 msec (for men)         or >470 msec (for women).     -   Gastrointestinal disease (eg, gastric or intestinal bypass         surgery, pancreatic enzyme insufficiency, malabsorption         syndrome, symptomatic inflammatory bowel disease, chronic         diarrheal illness, bowel obstruction) that might interfere with         drug absorption or with interpretation of gastrointestinal AEs.     -   Ongoing risk for bleeding due to active peptic ulcer disease;         bleeding diathesis; or requirement for systemic anticoagulation         with an antiplatelet drug (eg, aspirin, triflusal, clopidogrel,         prasugrel, ticagrelor, ticlopidine, cilostazol, vorapaxar,         dipyridamole); or with heparin, low-molecular-weight heparin or         heparin fractions (eg, enoxaparin, dalteparin, fondaparinux) or         oral anticoagulants (eg, apixaban, rivaroxaban, dabigatran         etexilate, warfarin). Note: Use of heparin or thrombolytic         agents for local maintenance or clearance of a central venous         catheter is permitted.     -   Evidence of an ongoing systemic bacterial, fungal, or viral         infection (including upper respiratory tract infections) at the         time of start of study therapy. Note: Subjects with localized         fungal infections of skin or nails are not precluded from         participation.     -   In subjects with prior hematopoietic progenitor cell         transplantation, evidence of ongoing graft-versus-host disease         (GVHD).     -   Pregnancy or breastfeeding.     -   Major surgery within 4 weeks before the start of study therapy.     -   Prior solid organ transplantation.     -   Prior anti-ROR1 therapy within 12 weeks prior to the start of         study therapy.     -   Ongoing immunosuppressive therapy, including systemic or enteric         corticosteroids. Note: At Screening, subjects may be using         topical or inhaled corticosteroids. During study therapy, use of         corticosteroids as prophylaxis for infusion reactions will be         minimized. However, subjects may use systemic, enteric, topical         or enteric corticosteroids as required for treatment-emergent         conditions

Primary Outcome Measures:

-   -   Phase 1b: Recommended dosing regimen (RDR) [Time Frame: From         baseline to 52 weeks] Evaluation of cirmtuzumab         dose-pharmacodynamic and pharmacokinetic-pharmacodynamic         relationships, and safety.     -   Phase 2: Complete Response (CR) rate [Time Frame: From         randomization to discontinuation of treatment of all study         subjects or 72 weeks after accrual of the final subject]         Proportion of subjects achieving a CR in accordance with         pre-established response criteria for lymphoma (Cheson, J Clin         Oncol. 32(27):3059-68 (2014)).

TABLE 11 Exemplary Dosing Regimens Arms Assigned Interventions Phase 1b - dose Administered IV every 2 weeks for 5 finding for administrations and then every 4 weeks ROR1-MMAE thereafter Phase 1b - dose Administered IV every 2 weeks for 3 expansion for administrations and then every 4 weeks ROR1-MMAE thereafter Phase 2 - safety and Administered IV every 2 weeks for 3 efficacy evaluation for administrations and then every 4 weeks ROR1-MMAE thereafter

Dose finding phase will be conducted by parallel dose comparison at 1.0, 2.0, and 3.0 mg/kg.

While preferred embodiments of the present invention have been shown and described herein, it will be obvious to those skilled in the art that such embodiments are provided by way of example only. Numerous variations, changes, and substitutions will now occur to those skilled in the art without departing from the invention. It should be understood that various alternatives to the embodiments of the invention described herein may be employed in practicing the invention.

The amino acid sequences and nucleotide sequences described herein are listed in Table 12 below.

TABLE 12 List of Sequences SIN Description SEQUENCE 1 Human ROR1 VATNGKEVVS STGVLFVKFG PC fragment 2 Human ROR1 EVVSSTGVLF VKFGPC fragment 3 Ab1 heavy QVQLQESGPGLVKPSQTLSLTCTVSGYAFTAYNIHWVRQAPGQGLEW chain MGSFDPYDGGSSYNQKFKDRLTISKDTSKNQVVLTMTNMDPVDTATY YCARGWYYFDYWGHGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAAL GCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSS SLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTCPPCPAPELLGGPSV FLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNA KTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEK TISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWES NGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHE ALHNHYTQKSLSLSPGK 4 Ab1 light DIVMTQTPLSLPVTPGEPASISCRASKSISKYLAWYQQKPGQAPRLLIYS chain GSTLQSGIPPRFSGSGYGTDFTLTINNIESEDAAYYFCQQHDESPYTFGE GTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWK VDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVT HQGLSSPVTKSFNRGEC 5 Ab1 VH QVQLQESGPGLVKPSQTLSLTCTVSGYAFTAYNIHWVRQAPGQGLEW MGSFDPYDGGSSYNQKFKDRLTISKDTSKNQVVLTMTNMDPVDTATY YCARGWYYFDYWGHGTLVTVSS 6 Ab1 VL DIVMTQTPLSLPVTPGEPASISCRASKSISKYLAWYQQKPGQAPRLLIYS GSTLQSGIPPRFSGSGYGTDFTLTINNIESEDAAYYFCQQHDESPYTFGE GTKVEIK 7 Ab1 HCDR1 GYAFTAYN 8 Ab1 HCDR2 FDPYDGGS 9 Ab1 HCDR3 GWYYFDY 10 Ab1 LCDR1 KSISKY 11 Ab1 LCDR2 SGS 12 Ab1 LCDR3 QQHDESPY 13 Ab1 VH frag. SGYAFTAYNIHWVRQ 14 Ab1 VH frag. GSFDPYDGGSSYNQKF 15 Ab1 VH frag. YYCARGWYYFDYWGHGTLVTVSS 16 Ab1 VL frag. CRASKSISKYLAWY 17 Ab1 VL frag. LLIYSGSTLQSG 18 Ab1 VL frag. CQQHDESPYTFGEGTKVEIK 19 Ab1 heavy AAGCTTACCGCCACCATGGGCTGGAGCTGTATCATCCTCTTCCTGGT chain coding GGCGACCGCGACGGGTGTCCACTCCCAGGTGCAGCTCCAGGAGTCC sequence GGCCCCGGGCTTGTGAAGCCGTCACAAACCCTGTCCCTGACGTGCA CGGTCTCCGGCTACGCCTTCACGGCCTACAACATACATTGGGTCCG GCAGGCGCCGGGCCAGGGGCTGGAGTGGATGGGTTCCTTCGACCCG TACGATGGCGGGAGCTCGTACAACCAGAAGTTCAAAGACCGCCTGA CGATCTCCAAGGACACCTCGAAAAACCAGGTCGTCCTGACCATGAC CAACATGGACCCGGTGGACACGGCGACCTACTATTGCGCCCGCGGC TGGTACTACTTCGACTACTGGGGCCACGGGACCCTGGTCACCGTGT CTTCCGCTTCGACCAAGGGCCCCAGCGTCTTCCCGCTCGCGCCCTCC TCGAAGTCCACCTCGGGCGGCACTGCCGCCCTGGGCTGCCTGGTCA AGGACTACTTCCCGGAGCCGGTGACCGTCTCGTGGAACAGCGGGGC ACTCACCTCCGGCGTGCACACCTTCCCGGCCGTGCTGCAGTCCTCGG GGCTGTATTCACTCAGCTCGGTCGTCACCGTCCCCTCGTCGTCCCTC GGCACGCAGACGTACATCTGCAACGTCAACCACAAGCCCTCGAACA CCAAGGTGGACAAGAAGGTCGAGCCGAAGTCCTGCGATAAGACCC ACACCTGCCCCCCGTGCCCGGCCCCCGAGCTCCTGGGCGGTCCGTC CGTGTTCCTCTTCCCGCCCAAGCCCAAGGACACCCTGATGATCAGCC GCACGCCCGAGGTGACCTGCGTCGTCGTGGACGTCTCCCACGAGGA TCCCGAGGTGAAGTTCAACTGGTACGTGGACGGGGTGGAGGTCCAC AACGCCAAGACAAAGCCGCGGGAAGAGCAGTACAACTCGACCTAC CGCGTCGTCAGCGTGCTGACGGTCCTCCACCAGGACTGGCTGAACG GCAAGGAGTACAAATGCAAGGTGTCCAACAAGGCCCTGCCCGCGCC CATCGAGAAGACCATCTCCAAGGCCAAGGGACAGCCGCGCGAGCC GCAGGTCTACACGCTGCCTCCCTCCCGGGACGAGCTCACGAAGAAC CAGGTATCGCTCACCTGCCTCGTGAAGGGCTTCTACCCGAGCGACA TCGCCGTCGAGTGGGAGAGCAACGGCCAGCCCGAGAACAACTACA AAACCACGCCGCCGGTCCTCGACTCTGACGGGTCCTTCTTCCTGTAC TCCAAGCTGACCGTGGACAAGTCGCGGTGGCAGCAGGGGAACGTGT TCTCGTGCTCGGTCATGCACGAGGCGTTGCACAACCACTACACCCA GAAGTCACTCTCCCTGAGCCCGGGCAAGTGATAATCTAGAGTCGGG GCGGCCGGCC 20 Ab1 light AAGCTTACCGCCACCATGGGCTGGTCATGCATCATCCTGTTCCTGGT chain coding CGCCACCGCGACGGGGGTCCACAGTGATATCGTCATGACGCAGACG sequence CCGCTGAGCCTCCCGGTGACGCCCGGCGAGCCCGCCAGCATCTCCT GCCGCGCTTCCAAGTCCATCTCGAAGTACCTGGCGTGGTATCAGCA GAAGCCCGGCCAGGCCCCGCGCCTGCTCATCTACTCTGGTTCCACG CTCCAGTCGGGCATCCCGCCCCGGTTCTCGGGTTCGGGATACGGCA CCGACTTCACCCTGACCATCAACAACATCGAGAGCGAAGACGCGGC GTACTACTTCTGCCAGCAGCACGACGAGTCCCCGTACACCTTCGGC GAGGGGACCAAGGTCGAGATCAAGCGTACCGTCGCGGCACCGAGC GTCTTCATCTTCCCCCCGTCCGACGAGCAGCTCAAGTCTGGCACCGC CTCGGTCGTCTGTCTCCTGAACAACTTCTACCCCAGGGAAGCCAAG GTCCAGTGGAAGGTGGACAACGCGCTGCAGTCCGGGAATAGCCAG GAGTCGGTGACGGAGCAGGACTCCAAGGACTCCACGTACTCGCTCT CGTCCACCCTGACCCTCTCCAAGGCGGACTACGAAAAGCACAAGGT CTACGCCTGCGAGGTGACGCACCAAGGCCTGTCCTCCCCAGTGACC AAGTCGTTCAACCGCGGCGAGTGCTGATAATCTAGAGTCGGGGCGG CCGGCC 21 Ab1 VH AAGCTTACCGCCACCATGGGCTGGAGCTGTATCATCCTCTTCCTGGT coding GGCGACCGCGACGGGTGTCCACTCCCAGGTGCAGCTCCAGGAGTCC sequence 1 GGCCCCGGGCTTGTGAAGCCGTCACAAACCCTGTCCCTGACGTGCA CGGTCTCCGGCTACGCCTTCACGGCCTACAACATACATTGGGTCCG GCAGGCGCCGGGCCAGGGGCTGGAGTGGATGGGTTCCTTCGACCCG TACGATGGCGGGAGCTCGTACAACCAGAAGTTCAAAGACCGCCTGA CGATCTCCAAGGACACCTCGAAAAACCAGGTCGTCCTGACCATGAC CAACATGGACCCGGTGGACACGGCGACCTACTATTGCGCCCGCGGC TGGTACTACTTCGACTACTGGGGCCACGGGACCCTGGTCACCGTGT CTTCC 22 Ab1 VL GATATCGTCATGACGCAGACGCCGCTGAGCCTCCCGGTGACGCCCG coding GCGAGCCCGCCAGCATCTCCTGCCGCGCTTCCAAGTCCATCTCGAA sequence 1 GTACCTGGCGTGGTATCAGCAGAAGCCCGGCCAGGCCCCGCGCCTG CTCATCTACTCTGGTTCCACGCTCCAGTCGGGCATCCCGCCCCGGTT CTCGGGTTCGGGATACGGCACCGACTTCACCCTGACCATCAACAAC ATCGAGAGCGAAGACGCGGCGTACTACTTCTGCCAGCAGCACGACG AGTCCCCGTACACCTTCGGCGAGGGGACCAAGGTCGAGATCAAG 23 Ab1 VH CAGGTGCAGCTGCAGGAGTCGGGCCCAGGACTGGTGAAGCCTTCAC coding AGACCCTGTCCCTCACCTGCACTGTCTCTGGTTATGCATTCACTGCC sequence 2 TACAACATACACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGT GGATGGGTTCTTTTGATCCTTACGATGGTGGTAGTAGTTACAACCAG AAGTTCAAGGACAGACTCACCATCTCCAAGGACACCTCCAAAAACC AGGTGGTCCTTACAATGACCAACATGGACCCTGTGGACACAGCCAC GTATTACTGTGCAAGAGGGTGGTACTACTTTGACTACTGGGGCCAC GGAACCCTGGTCACCGTCTCCTCA 24 Ab1 VL GATATTGTGATGACCCAGACTCCACTCTCCCTGCCCGTCACCCCTGG coding AGAGCCGGCCTCCATCTCCTGCAGGGCAAGTAAGAGCATTAGCAAA sequence 2 TATTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCC TCATCTATTCTGGATCCACTTTGCAATCTGGGATCCCACCTCGATTC AGTGGCAGCGGGTATGGAACAGATTTTACCCTCACAATTAATAACA TAGAATCTGAGGATGCTGCATATTACTTCTGTCAACAGCATGATGA ATCCCCGTACACGTTCGGCGAGGGGACCAAGGTGGAAATCAAA 25 D10 VH QVQLKESGPGLVAPSQTLSITCTVSGFSLTSYGVHWVRQPPGKGLEWL GVIWAGGFTNYNSALKSRLSISKDNSKSQVLLKMTSLQTDDTAMYYC ARRGSSYSMDYWGQGTSVIVSS 26 D10 VL EIVLSQSPAITAASLGQKVTITCSASSNVSYIHWYQQRSGTSPRPWIYEIS KLASGVPVRFSGSGSGTSYSLTISSMEAEDAAIYYCQQWNYPLITFGSG TKLEIQ 27 D10 HCDR1 GFSLTSYG 28 D10 HCDR2 WAGGFT 29 D10 HCDR3 RGSSYSMDY 30 D10 LCDR1 SNVSYI 31 D10 LCDR2 EIS 32 D10 LCDR3 QQWNYPLI 33 D10 VH CAGGTGCAGCTGAAGGAGTCAGGACCTGGCCTGGTGGCGCCCTCAC coding AGACTCTGTCCATCACTTGCACTGTCTCTGGGTTTTCATTAACCAGT sequence TATGGTGTACACTGGGTTCGCCAGCCTCCAGGAAAGGGTCTGGAGT GGCTGGGAGTAATATGGGCTGGTGGATTCACAAATTATAATTCGGC TCTCAAGTCCAGACTGAGCATCAGCAAAGACAACTCCAAGAGCCAA GTTCTCTTAAAAATGACCAGTCTGCAAACTGATGACACAGCCATGT ACTACTGTGCCAGGAGAGGTAGTTCCTATTCTATGGACTATTGGGGT CAAGGAACCTCAGTCACCGTCTCCTCA 34 D10 VL GAAATTGTGCTCTCTCAGTCTCCAGCCATCACAGCTGCATCTCTGGG coding CCAAAAGGTCACCATCACCTGCAGTGCCAGTTCAAATGTAAGTTAC sequence ATCCACTGGTACCAGCAGAGGTCAGGCACCTCCCCCAGACCATGGA TTTATGAAATATCCAAACTGGCTTCTGGAGTCCCAGTTCGCTTCAGT GGCAGTGGGTCTGGGACCTCTTACTCTCTCACAATCAGCAGCATGG AGGCTGAAGATGCTGCCATTTATTATTGTCAGCAGTGGAATTATCCT CTTATCACGTTCGGCTCGGGGACAAAGTTGGAAATACAA 35 4A5 VH EVKLVESGGGLVKPGGSLKLSCAASGFTFSSYAMSWVRQIPEKRLEWV ASISRGGTTYYPDSVKGRFTISRDNVRNILYLQMSSLRSEDTAMYYCGR YDYDGYYAMDYWGQGTSVTVSS 36 4A5 VL DIKMTQSPSSMYASLGERVTTTCKASPDINSYLSWFQQKPGKSPKTLIY RANRLVDGVPSRFSGGGSGQDYSLTINSLEYEDMGIYYCLQYDEFPYT FGGTKLEMK 37 4A5 HEDR1 GFTFSSYA 38 4A5 HCDR2 ISRGGTT 39 4A5 HCDR3 YDYDGYYAMDY 40 4A5 LCDR1 PDINSY 41 4A5 LCDR2 RAN 42 4A5 LCDR3 LQYDEFPYT 43 4A5 VH GAAGTGAAACTGGTGGAGTCTGGGGGAGGCTTAGTGAAGCCTGGA coding GGGTCCCTGAAACTCTCCTGTGCAGCCTCTGGATTCACTTTCAGTAG sequence CTATGCCATGTCTTGGGTTCGCCAGATTCCAGAGAAGAGGCTGGAG TGGGTCGCATCCATTAGTCGTGGTGGTACCACCTACTATCCAGACA GTGTGAAGGGCCGATTCACCATCTCCAGAGATAATGTCAGGAACAT CCTGTACCTGCAAATGAGCAGTCTGAGGTCTGAGGACACGGCCATG TATTACTGTGGAAGATATGATTACGACGGGTACTATGCAATGGACT ACTGGGGTCAAGGAACCTCAGTCACCGTCTCCTCA 44 4A5 VL GACATCAAGATGACCCAGTCTCCATCTTCCATGTATGCATCTCTAGG coding AGAGAGAGTCACTATCACTTGCAAGGCGAGTCCGGACATTAATAGC sequence TATTTAAGCTGGTTCCAGCAGAAACCAGGGAAATCTCCTAAGACCC TGATCTATCGTGCAAACAGATTGGTTGATGGGGTCCCATCAAGGTT CAGTGGCGGTGGATCTGGGCAAGATTATTCTCTCACCATCAACAGC CTGGAGTATGAAGATATGGGAATTTATTATTGTCTACAGTATGATG AATTTCCGTACACGTTCGGAGGGGGGACCAAGCTGGAAATGAAAC 45 99961 VH EIQLQQSGPVLVKPGASVKVSCKASGYAFTAYNIHWVRQSHGKRLEWI GSFDPYDGGSSYNQKFKDKATLTVDKSSTTAYMHLNSLTSEDSAVYY CARGWYYFDYWGHGTTLTVSS 46 99961 VL DVQITQSPSYLAASPGETITINCRASKSISKYLAWYQEKPGKTNKLLIYS GSTLQSGIPSRFRGSGSGTDFTLTISSLEPEDFAMYYCQQHDESPYTFGE GTKLEIKR 47 Ab4 heavy QVQLQESGPGLVKPSQTLSLTCTVSGYAFTAYNIHWIRQPPGKGLEWIG chain SFDPYDGGSSYNQKFKDRLTISKDTSKNQVVLTMTNMDPVDTATYYC ARGWYYFDYWGHGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCL VKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLG TQTYICNVNHKPSNTKVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLF PPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTK PREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISK AKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQ PENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALH NHYTQKSLSLSPGK 48 Ab4 VH QVQLQESGPGLVKPSQTLSLTCTVSGYAFTAYNIHWIRQPPGKGLEWIG SFDPYDGGSSYNQKFKDRLTISKDTSKNQVVLTMTNMDPVDTATYYC ARGWYYFDYWGHGTLVTVSS 49 Ab4 light DVVMTQSPLSLPVTLGQPASISCRASKSISKYLAWYQQKPGKAPKLLIY chain SGSTLQSGIPPRFSGSGYGTDFTLTINNIESEDAAYYFCQQHDESPYTFG EGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQW KVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEV THQGLSSPVTKSFNRGEC 50 Ab4 VL DVVMTQSPLSLPVTLGQPASISCRASKSISKYLAWYQQKPGKAPKLLIY SGSTLQSGIPPRFSGSGYGTDFTLTINNIESEDAAYYFCQQHDESPYTFG EGTKVEIK 51 Ab4 heavy CAGGTCCAGCTGCAGGAGTCAGGTCCCGGACTGGTCAAGCCGTCGC chain coding AGACGCTGTCCCTCACCTGCACCGTGTCGGGCTACGCCTTCACCGCC sequence TACAACATCCACTGGATCCGTCAGCCCCCTGGGAAGGGCCTCGAGT GGATCGGCAGCTTCGACCCGTACGACGGCGGGTCGTCCTACAACCA GAAGTTCAAGGACCGCCTCACCATCAGCAAGGACACCTCCAAGAAC CAGGTCGTCCTCACCATGACCAACATGGACCCCGTGGACACCGCCA CGTACTACTGCGCGCGGGGCTGGTACTACTTCGACTACTGGGGGCA CGGCACCCTCGTCACGGTCTCGTCGGCGAGCACCAAGGGTCCGAGC GTCTTCCCCCTGGCCCCCTCCAGCAAGTCCACCTCGGGGGGCACCG CCGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAGCCCGTGAC CGTGAGCTGGAACTCCGGCGCCCTCACCAGCGGGGTCCACACCTTC CCGGCGGTCCTGCAGTCATCCGGTCTCTACTCCTTGAGCTCAGTCGT CACCGTCCCGAGCTCCTCCCTCGGAACGCAGACCTACATCTGCAAC GTCAACCACAAGCCGTCCAACACCAAGGTCGACAAGAAGGTGGAG CCCAAATCGTGCGACAAGACCCACACCTGCCCGCCGTGCCCCGCCC CGGAACTGCTCGGCGGCCCCTCGGTGTTCCTGTTCCCCCCGAAGCCC AAGGACACCCTCATGATCTCCCGCACCCCCGAGGTCACCTGCGTGG TGGTGGATGTCTCCCACGAGGACCCCGAGGTGAAGTTCAACTGGTA CGTGGACGGGGTCGAGGTGCACAACGCCAAGACCAAGCCCCGAGA GGAACAGTATAACTCGACGTACCGCGTGGTCAGCGTCCTGACCGTG CTCCACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTC AGCAACAAGGCCCTGCCCGCCCCCATCGAGAAGACGATCTCCAAGG CGAAGGGGCAGCCGCGCGAGCCGCAGGTCTACACCCTGCCGCCCAG CCGGGACGAGCTCACGAAGAATCAGGTCTCGCTCACCTGCCTCGTC AAGGGTTTCTACCCGTCGGACATCGCGGTCGAATGGGAGTCGAACG GTCAGCCCGAGAATAACTACAAGACGACCCCGCCCGTCCTGGACTC GGACGGCAGCTTCTTCCTGTACTCGAAGCTGACGGTCGACAAGTCG CGCTGGCAGCAGGGCAACGTCTTCTCGTGCTCGGTGATGCACGAGG CCCTCCACAACCACTACACACAGAAGAGCCTCTCGCTTTCGCCGGG CAAG 52 Ab4 VH CAGGTCCAGCTGCAGGAGTCAGGTCCCGGACTGGTCAAGCCGTCGC coding AGACGCTGTCCCTCACCTGCACCGTGTCGGGCTACGCCTTCACCGCC sequence TACAACATCCACTGGATCCGTCAGCCCCCTGGGAAGGGCCTCGAGT GGATCGGCAGCTTCGACCCGTACGACGGCGGGTCGTCCTACAACCA GAAGTTCAAGGACCGCCTCACCATCAGCAAGGACACCTCCAAGAAC CAGGTCGTCCTCACCATGACCAACATGGACCCCGTGGACACCGCCA CGTACTACTGCGCGCGGGGCTGGTACTACTTCGACTACTGGGGGCA CGGCACCCTCGTCACGGTCTCGTCG 53 Ab4 light GACGTCGTGATGACCCAGTCGCCCCTCTCCCTGCCGGTTACCCTGGG chain coding CCAGCCCGCCTCCATCAGCTGCCGTGCCTCCAAGTCCATTTCCAAGT sequence ACCTGGCCTGGTACCAGCAGAAGCCGGGGAAGGCCCCAAAGCTCCT CATCTACTCCGGCTCCACCCTCCAGAGCGGCATCCCCCCCCGCTTCA GCGGCTCCGGCTACGGCACCGACTTCACCCTCACCATCAATAACAT CGAGTCGGAGGACGCCGCGTACTACTTCTGCCAGCAGCACGACGAA TCGCCGTACACCTTCGGGGAGGGCACCAAGGTGGAGATCAAGAGG ACGGTCGCCGCGCCCTCCGTGTTCATCTTCCCCCCCTCGGACGAACA GCTGAAGTCCGGGACCGCCTCCGTCGTCTGCCTCCTCAACAACTTCT ACCCGCGCGAGGCCAAGGTGCAGTGGAAGGTCGACAACGCGCTCC AGTCCGGCAACTCCCAGGAGTCGGTGACCGAGCAGGACTCGAAGG ACAGTACCTACTCGCTGAGCTCCACACTGACGCTCTCGAAGGCCGA CTACGAGAAGCACAAGGTGTACGCATGCGAGGTGACCCACCAGGG GCTGAGCTCGCCGGTGACTAAGTCGTTCAACAGGGGCGAATGC 54 Ab4 VL GACGTCGTGATGACCCAGTCGCCCCTCTCCCTGCCGGTTACCCTGGG coding CCAGCCCGCCTCCATCAGCTGCCGTGCCTCCAAGTCCATTTCCAAGT sequence ACCTGGCCTGGTACCAGCAGAAGCCGGGGAAGGCCCCAAAGCTCCT CATCTACTCCGGCTCCACCCTCCAGAGCGGCATCCCCCCCCGCTTCA GCGGCTCCGGCTACGGCACCGACTTCACCCTCACCATCAATAACAT CGAGTCGGAGGACGCCGCGTACTACTTCTGCCAGCAGCACGACGAA TCGCCGTACACCTTCGGGGAGGGCACCAAGGTGGAGATCAAG 55 Linker GGFG peptide moiety sequence 56 Linker ALAL peptide moiety sequence 57 Linker GFLG peptide moiety sequence 58 Glutamine tag LLQGA 59 Sortase motif LPxTG 60 Sortase motif NPQTN 61 BirA tag GFEIDKVWYDLDA 62 Human CH₁ ASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSG domain plus ALT SGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKP upper hinge SNTKVDKKVEPKSC region 63 Human Kappa RTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVD constant NALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACE domain VTHQGLSSPVTKSFNRGEC 64 Humanized DIQMTQSPSSLSASVGDRVTITCKASPDINSYLSWFQQRPGQSPR 4A5 scFv RLIYRANRLVDGVPDRFSGSGSGTDFTLKISRVEAEDVGVYYCL QYDEFPYTFGQGTKVEIKGGGGSGSTSGSGKPGSGEGSTKGGGG GSEVQLVQSGAEVKKPGESLRISCKGSGFTFSSYAMSWIRQSPSR GLEWLGSISRGGTTYYPDSVKGRFTISRDNAKNSLYLQMNSLRA EDTAVYYCGRYDYDGYYAMDYWGQGTLVTVSS 65 Ab1 scFv DIVMTQTPLSLPVTPGEPASISCRASKSISKYLAWYQQKPGQAPR LLIYSGSTLQSGIPPRFSGSGYGTDFTLTINNIESEDAAYYFCQQH DESPYTFGEGTKVEIKGGGGSGSTSGSGKPGSGEGSTKGGGGGS QVQLQESGPGLVKPSQTLSLTCTVSGYAFTAYNIHWVRQAPGQG LEWMGSFDPYDGGSSYNQKFKDRLTISKDTSKNQVVLTMTNMD PVDTATYYCARGWYYFDYWGHGTLVTVSS 66 Ab2 scFv DVVMTQSPLSLPVTLGQPASISCRASKSISKYLAWYQQKPGKAP KLLIYSGSTLQSGIPPRFSGSGYGTDFTLTINNIESEDAAYYFCQQ HDESPYTFGEGTKVEIKGGGGSGSTSGSGKPGSGEGSTKGGGGG SQVQLQESGPGLVKPSQTLSLTCTVSGYAFTAYNIHWVRQAPGQ GLEWMGSFDPYDGGSSYNQKFKDRLTISKDTSKNQVVLTMTNM DPVDTATYYCARGWYYFDYWGHGTLVTVSS 67 Ab3 scFv DIVMTQTPLSLPVTPGEPASISCRASKSISKYLAWYQQKPGQAPR LLIYSGSTLQSGIPPRFSGSGYGTDFTLTINNIESEDAAYYFCQQH DESPYTFGEGTKVEIKGGGGSGSTSGSGKPGSGEGSTKGGGGGS QVQLQESGPGLVKPSQTLSLTCTVSGYAFTAYNIHWIRQPPGKG LEWIGSFDPYDGGSSYNQKFKDRLTISKDTSKNQVVLTMTNMDP VDTATYYCARGWYYFDYWGHGTLVTVSS 68 Ab4 scFv DVVMTQSPLSLPVTLGQPASISCRASKSISKYLAWYQQKPGKAP KLLIYSGSTLQSGIPPRFSGSGYGTDFTLTINNIESEDAAYYFCQQ HDESPYTFGEGTKVEIKGGGGSGSTSGSGKPGSGEGSTKGGGGG SQVQLQESGPGLVKPSQTLSLTCTVSGYAFTAYNIHWIRQPPGKG LEWIGSFDPYDGGSSYNQKFKDRLTISKDTSKNQVVLTMTNMDP VDTATYYCARGWYYFDYWGHGTLVTVSS *SIN: SEQ ID NO. 

1-41. (canceled)
 42. A method of treating a ROR1-expressing hematological cancer in a patient in need thereof, comprising administering to the patient an immunoconjugate comprising an antibody conjugated to a cytotoxic drug moiety, wherein the V_(H) and V_(L) of the antibody comprise the amino acid sequences of SEQ ID NOs: 5 and 6, respectively, and wherein the cytotoxic drug moiety is (i) monomethyl auristatin E (MMAE) and is conjugated to the antibody at a lysine residue via a linker comprising valine-citrulline (VC) and para-amino-benzyloxycarbonyl (PAB) moieties; (ii) MMAE and is conjugated to the antibody at a cysteine residue via a linker comprising diaminopropionic acid (DPR), VC, and PAB moieties; (iii) MMAE and is conjugated to the antibody at a cysteine residue via a linker comprising benzyl (Phe)-C₄, VC, and PAB moieties; (iv) MMAE and is conjugated to the antibody at a glutamine residue via a branched linker comprising dibenzylcyclooctyne (DBCO), polyethylene glycol (PEG)₂, VC, and PAB moieties; (v) azonafide and is conjugated to the antibody at a cysteine residue via a linker comprising VC and PAB moieties; (vi) duocarmycin and is conjugated to the antibody at a cysteine residue via a linker comprising PEG₄, VC, PAB, and dimethylethylamine (DMEA) moieties; (vii) pyrrolobenzodiazepine (PBD) and is conjugated to the antibody at a cysteine residue via a linker comprising PEG₄ and valine-alanine (VA) moieties; (viii) PNU-159682 and is conjugated to the antibody at a cysteine residue via a linker comprising PEG₄, VC, PAB, and DMEA moieties; (ix) PNU-159682 and is conjugated to the antibody at a cysteine residue via a linker comprising C₂-glycine (Gly)₃, and ethylene diamine (EDA) moieties; (x) PNU-159682 and is conjugated to the antibody at a cysteine residue via a linker comprising Phe-C₄, VC, PAB, and DMEA moieties; or (xi) PNU-159682 and is conjugated to the antibody at a glutamine residue via a linker comprising PEG₄, VC, PAB, and DMEA moieties.
 43. The method of claim 42, wherein the heavy chain and light chain of the antibody comprise the amino acid sequences of SEQ ID NOs: 3 and 4, respectively.
 44. The method of claim 42, wherein the ratio of the cytotoxic drug moiety to the antibody is 1 to
 7. 45. The method of claim 42, wherein the hematological cancer is a lymphoma.
 46. The method of claim 42, wherein the hematological cancer is a leukemia.
 47. The method of claim 42, wherein the hematological cancer is a non-Hodgkin lymphoma.
 48. The method of claim 47, wherein the non-Hodgkin lymphoma is selected from diffuse large B-cell lymphoma, follicular lymphoma, mantle cell lymphoma, acute myeloid lymphoma, marginal zone lymphoma, and any non-Hodgkin lymphoma that has undergone Richter's transformation.
 49. The method of claim 42, further comprising administering an additional therapeutic agent selected from the group consisting of a Bruton's tyrosine kinase (BTK) inhibitor, a B-cell lymphoma 2 (Bcl-2) inhibitor, a mammalian target of rapamycin (mTOR) inhibitor, and a phosphoinositide 3-kinase (PI3K) inhibitor.
 50. The method of claim 49, wherein the additional therapeutic agent is selected from the group consisting of ibrutinib, acalabrutinib, venetoclax, everolimus, sapanisertib, and idelalisib.
 51. The method of claim 42, wherein the immunoconjugate has the structure

wherein Ab is the antibody and the linker is conjugated to the antibody at a lysine residue.
 52. The method of claim 42, wherein the immunoconjugate has the structure

wherein Ab is the antibody and the linker is conjugated to the antibody at a cysteine residue.
 53. The method of claim 42, wherein the immunoconjugate has the structure

wherein Ab is the antibody and the linker is conjugated to the antibody at a cysteine residue.
 54. The method of claim 42, wherein the immunoconjugate has the structure

wherein Ab is the antibody and the linker is conjugated to the antibody at a cysteine residue.
 55. The method of claim 42, wherein the immunoconjugate has the structure

wherein Ab is the antibody and the linker is conjugated to the antibody at a cysteine residue.
 56. The method of claim 42, wherein the immunoconjugate has the structure

wherein Ab is the antibody and the linker is conjugated to the antibody at a cysteine residue.
 57. The method of claim 42, wherein the immunoconjugate has the structure

wherein Ab is the antibody and the linker is conjugated to the antibody at a cysteine residue.
 58. The method of claim 42, wherein the immunoconjugate has the structure

wherein Ab is the antibody and the linker is conjugated to the antibody at a cysteine residue.
 59. The method of claim 42, wherein the immunoconjugate has the structure

wherein Ab is the antibody and the linker is conjugated to the antibody at a cysteine residue.
 60. The method of claim 42, wherein the immunoconjugate has the structure

wherein Ab is the antibody and the linker is conjugated to the antibody at a glutamine residue.
 61. A method of treating a ROR1-expressing hematological cancer in a patient in need thereof, comprising administering to the patient an immunoconjugate comprising an antibody conjugated to a cytotoxic drug moiety, wherein the V_(H) and V_(L) of the antibody comprise the amino acid sequences of SEQ ID NOs: 5 and 6, respectively, and wherein the cytotoxic drug moiety (D) is conjugated to the antibody as shown in the following structure

wherein Ab is the antibody, the linker is conjugated to the antibody at a lysine residue, and the cytotoxic drug moiety (D) is MMAE 